Alanine dehydrogenase - Phormidium lapideum

Preferred order of sections

Names and origin

Protein names

Recommended name:
Alanine dehydrogenase PIRNR PIRNR000183
EC=1.4.1.1 PIRNR PIRNR000183

Gene names

Name:

ald EMBL BAA24455.1

Organism

Phormidium lapideum EMBL BAA24455.1

Taxonomic identifier

32060 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Oscillatoriales › Phormidium

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-alanine + H₂O + NAD⁺ = pyruvate + NH₃ + NADH. PIRNR PIRNR000183
Pathway	Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step 1/1. PIRNR PIRNR000183
Sequence similarities	Belongs to the AlaDH/PNT family. PIRNR PIRNR000183

Ontologies

Keywords
Ligand	NAD PIRNR PIRNR000183 PDB 1PJC Nucleotide-binding PDB 1PJC
Molecular function	Oxidoreductase PIRNR PIRNR000183 EMBL BAA24455.1
Technical term	3D-structure PDB 1SAY PDB 1PJC PDB 1PJB
Gene Ontology (GO)
Molecular function	alanine dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Nucleotide binding

177 – 178

2

NAD PDB 1PJC

Sites

Binding site

133

1

NAD PDB 1PJC

Binding site

197

1

NAD PDB 1PJC

Binding site

219

1

NAD PDB 1PJC

Binding site

238

1

NAD; via carbonyl oxygen PDB 1PJC

Binding site

266

1

NAD; via carbonyl oxygen PDB 1PJC

Binding site

270

1

NAD PDB 1PJC

Binding site

300

1

NAD; via amide nitrogen PDB 1PJC

Sequences

Sequence

Length

Mass (Da)

Tools

O52942 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 57CA14BB85DB4D08
Show »

FASTA

361

38,557

        10         20         30         40         50         60 
MEIGVPKEIK NQEFRVGLSP SSVRTLVEAG HTVFIETQAG IGAGFADQDY VQAGAQVVPS 

        70         80         90        100        110        120 
AKDAWSREMV VKVKEPLPAE YDLMQKDQLL FTYLHLAAAR ELTEQLMRVG LTAIAYETVE 

       130        140        150        160        170        180 
LPNRSLPLLT PMSIIAGRLS VQFGARFLER QQGGRGVLLG GVPGVKPGKV VILGGGVVGT 

       190        200        210        220        230        240 
EAAKMAVGLG AQVQIFDINV ERLSYLETLF GSRVELLYSN SAEIETAVAE ADLLIGAVLV 

       250        260        270        280        290        300 
PGRRAPILVP ASLVEQMRTG SVIVDVAVDQ GGCVETLHPT SHTQPTYEVF GVVHYGVPNM 

       310        320        330        340        350        360 
PGAVPWTATQ ALNNSTLPYV VKLANQGLKA LETDDALAKG LNVQAHRLVH PAVQQVFPDL 


A

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References

[1]	"Alanine Dehydrogenase from a Cyanobacterium, Phormidium lapideum: Cloning, Sequencing, Expression, and Sequence Comparisons to Amino Acid Dehydrogenases." Sawa Y., Noshiro Y., Midorikawa Y., Sasai R., Murata K., Ashida H., Shibata H., Ochiai H. Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Purification and characterization of alanine dehydrogenase from a cyanobacterium, Phormidium lapideum." Sawa Y., Tani M., Murata K., Shibata H., Ochiai H. J. Biochem. 116:995-1000(1994) [PubMed: 7896761] [Abstract] Cited for: PROTEIN SEQUENCE.
[3]	"Analysis of the structure and substrate binding of Phormidium lapideum alanine dehydrogenase." Baker P.J., Sawa Y., Shibata H., Sedelnikova S.E., Rice D.W. Nat. Struct. Biol. 5:561-567(1998) [PubMed: 9665169] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH NAD.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D37807 Genomic DNA. Translation: BAA24455.1.

PIR

PX0078.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PJB	X-ray	2.10	A	1-361	[»]
1PJC	X-ray	2.00	A	1-361	[»]
1SAY	X-ray	2.10	A	1-361	[»]

ProteinModelPortal

O52942.

SMR

O52942. Positions 1-361.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008143. Ala_DH/PNT_CS2.
IPR008141. Ala_DH/Pyr_transHydgase.
IPR007886. AlaDH/PNT_N.
IPR007698. AlaDH/PNT_NAD(H)-bd.
[Graphical view]

PANTHER

PTHR10160:SF3. PTHR10160:SF3. 1 hit.

Pfam

PF01262. AlaDh_PNT_C. 1 hit.
PF05222. AlaDh_PNT_N. 1 hit.
[Graphical view]

PIRSF

PIRSF000183. Alanine_dh. 1 hit.

SMART

SM01002. AlaDh_PNT_C. 1 hit.
SM01003. AlaDh_PNT_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00518. AlaDH. 1 hit.

PROSITE

PS00837. ALADH_PNT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

O52942_PHOLP

Accession

Primary (citable) accession number: O52942

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 1998
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)