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O52942

- O52942_PHOLP

UniProt

O52942 - O52942_PHOLP

Protein

Alanine dehydrogenase

Gene

ald

Organism
Phormidium lapideum
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151PyruvateImported
    Binding sitei74 – 741PyruvateImported
    Sitei74 – 741Important for catalytic activityImported
    Sitei95 – 951Important for catalytic activityImported
    Sitei117 – 1171Important for catalytic activityImported
    Binding sitei133 – 1331NAD
    Binding sitei197 – 1971NAD
    Binding sitei219 – 2191NAD
    Binding sitei238 – 2381NAD; via carbonyl oxygen
    Binding sitei266 – 2661NAD; via carbonyl oxygen
    Sitei269 – 2691Important for catalytic activityImported
    Binding sitei270 – 2701NAD
    Binding sitei300 – 3001NAD; via amide nitrogen

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi177 – 1782NAD

    GO - Molecular functioni

    1. alanine dehydrogenase activity Source: UniProtKB-EC
    2. nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    1. L-alanine catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotationImported

    Keywords - Ligandi

    NADUniRule annotationImported, Nucleotide-bindingImported, PyruvateImported

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12816.
    UniPathwayiUPA00527; UER00585.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine dehydrogenaseUniRule annotation (EC:1.4.1.1UniRule annotation)
    Gene namesi
    Name:aldImported
    OrganismiPhormidium lapideumImported
    Taxonomic identifieri32060 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesPhormidium

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PJBX-ray2.10A1-361[»]
    1PJCX-ray2.00A1-361[»]
    1SAYX-ray2.10A1-361[»]
    ProteinModelPortaliO52942.
    SMRiO52942. Positions 1-361.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO52942.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni93 – 953Pyruvate bindingImported

    Sequence similaritiesi

    Belongs to the AlaDH/PNT family.UniRule annotation

    Family and domain databases

    InterProiIPR008141. Ala_DH.
    IPR008143. Ala_DH/PNT_CS2.
    IPR007886. AlaDH/PNT_N.
    IPR007698. AlaDH/PNT_NAD(H)-bd.
    [Graphical view]
    PfamiPF01262. AlaDh_PNT_C. 1 hit.
    PF05222. AlaDh_PNT_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000183. Alanine_dh. 1 hit.
    SMARTiSM01002. AlaDh_PNT_C. 1 hit.
    SM01003. AlaDh_PNT_N. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00518. alaDH. 1 hit.
    PROSITEiPS00837. ALADH_PNT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O52942-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEIGVPKEIK NQEFRVGLSP SSVRTLVEAG HTVFIETQAG IGAGFADQDY    50
    VQAGAQVVPS AKDAWSREMV VKVKEPLPAE YDLMQKDQLL FTYLHLAAAR 100
    ELTEQLMRVG LTAIAYETVE LPNRSLPLLT PMSIIAGRLS VQFGARFLER 150
    QQGGRGVLLG GVPGVKPGKV VILGGGVVGT EAAKMAVGLG AQVQIFDINV 200
    ERLSYLETLF GSRVELLYSN SAEIETAVAE ADLLIGAVLV PGRRAPILVP 250
    ASLVEQMRTG SVIVDVAVDQ GGCVETLHPT SHTQPTYEVF GVVHYGVPNM 300
    PGAVPWTATQ ALNNSTLPYV VKLANQGLKA LETDDALAKG LNVQAHRLVH 350
    PAVQQVFPDL A 361
    Length:361
    Mass (Da):38,557
    Last modified:June 1, 1998 - v1
    Checksum:i57CA14BB85DB4D08
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D37807 Genomic DNA. Translation: BAA24455.1.
    PIRiPX0078.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D37807 Genomic DNA. Translation: BAA24455.1 .
    PIRi PX0078.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PJB X-ray 2.10 A 1-361 [» ]
    1PJC X-ray 2.00 A 1-361 [» ]
    1SAY X-ray 2.10 A 1-361 [» ]
    ProteinModelPortali O52942.
    SMRi O52942. Positions 1-361.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00527 ; UER00585 .
    BioCyci MetaCyc:MONOMER-12816.

    Miscellaneous databases

    EvolutionaryTracei O52942.

    Family and domain databases

    InterProi IPR008141. Ala_DH.
    IPR008143. Ala_DH/PNT_CS2.
    IPR007886. AlaDH/PNT_N.
    IPR007698. AlaDH/PNT_NAD(H)-bd.
    [Graphical view ]
    Pfami PF01262. AlaDh_PNT_C. 1 hit.
    PF05222. AlaDh_PNT_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000183. Alanine_dh. 1 hit.
    SMARTi SM01002. AlaDh_PNT_C. 1 hit.
    SM01003. AlaDh_PNT_N. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00518. alaDH. 1 hit.
    PROSITEi PS00837. ALADH_PNT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Purification and characterization of alanine dehydrogenase from a cyanobacterium, Phormidium lapideum."
      Sawa Y., Tani M., Murata K., Shibata H., Ochiai H.
      J. Biochem. 116:995-1000(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
    2. "Alanine Dehydrogenase from a Cyanobacterium, Phormidium lapideum: Cloning, Sequencing, Expression, and Sequence Comparisons to Amino Acid Dehydrogenases."
      Sawa Y., Noshiro Y., Midorikawa Y., Sasai R., Murata K., Ashida H., Shibata H., Ochiai H.
      Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
    3. "Analysis of the structure and substrate binding of Phormidium lapideum alanine dehydrogenase."
      Baker P.J., Sawa Y., Shibata H., Sedelnikova S.E., Rice D.W.
      Nat. Struct. Biol. 5:561-567(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH NAD AND PYRUVATE, ACTIVE SITE.

    Entry informationi

    Entry nameiO52942_PHOLP
    AccessioniPrimary (citable) accession number: O52942
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 1, 1998
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3