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O52914

- SYE2_CAMJE

UniProt

O52914 - SYE2_CAMJE

Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei243 – 2431ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCJEJ192222:GJTS-1261-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetase 2UniRule annotation
    Short name:
    GluRS 2UniRule annotation
    Gene namesi
    Name:gltX2UniRule annotation
    Synonyms:gltX1
    Ordered Locus Names:Cj1288c
    OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
    Taxonomic identifieri192222 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
    ProteomesiUP000000799: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 463463Glutamate--tRNA ligase 2PRO_0000119531Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    IntActiO52914. 32 interactions.
    STRINGi192222.Cj1288c.

    Structurei

    3D structure databases

    ProteinModelPortaliO52914.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi11 – 2111"HIGH" regionAdd
    BLAST
    Motifi240 – 2445"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252722.
    KOiK01885.
    OMAiVTGQTHG.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O52914-1 [UniParc]FASTAAdd to Basket

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    MHEKLTTRFA PSPTGYLHIG GLRTALYNYL YARKNGGNFL LRIEDTDLKR    50
    NSKEATKAII EAFKWCGLEH DGEVTYQSER FDLYKEYVKK LLDEGKAYYC 100
    YMSKEELEEL RAKQEAAKER PRYDGRYREF TGTPPQGIEP VVRIKAPQSG 150
    EIVFEDGVKG EVRFKAEDIM DDFIIARSDG TPTYNFTVVI DDALMGVSDV 200
    IRGDDHLSNT PKQIVLYEAL GFKIPKFFHV AMIHGEDGKK LSKRHGATDV 250
    MEYKEMGILP QALLNFLVRL GWSHGDDEVF SLEDLKKLFD PYHINKSASC 300
    YNAKKLEWLN AHYIKTLPFE EINRQLKDLG FDLSVYEKAG FLLDLLRERA 350
    KTLHDIINGA KSIVNAPQNY DENAVQKFVN KNNLELLQAF ANTLKDQKTG 400
    KDFEDFTNDF LEKKEAKLKD LAQPIRIALT GSAVSPSIFE VLEFLGVDEC 450
    KKRIDNFLKV RGK 463
    Length:463
    Mass (Da):53,059
    Last modified:December 1, 2000 - v2
    Checksum:iD60A0032963E6BED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL111168 Genomic DNA. Translation: CAL35402.1.
    AJ002027 Genomic DNA. Translation: CAA05150.1.
    PIRiA81337.
    RefSeqiYP_002344678.1. NC_002163.1.

    Genome annotation databases

    EnsemblBacteriaiCAL35402; CAL35402; Cj1288c.
    GeneIDi905580.
    KEGGicje:Cj1288c.
    PATRICi20059540. VBICamJej33762_1270.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL111168 Genomic DNA. Translation: CAL35402.1 .
    AJ002027 Genomic DNA. Translation: CAA05150.1 .
    PIRi A81337.
    RefSeqi YP_002344678.1. NC_002163.1.

    3D structure databases

    ProteinModelPortali O52914.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O52914. 32 interactions.
    STRINGi 192222.Cj1288c.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL35402 ; CAL35402 ; Cj1288c .
    GeneIDi 905580.
    KEGGi cje:Cj1288c.
    PATRICi 20059540. VBICamJej33762_1270.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252722.
    KOi K01885.
    OMAi VTGQTHG.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci CJEJ192222:GJTS-1261-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCTC 11168.
    2. Woesten M.M.S.M., Boeve M., Koot M.G.A., Nuenen A.C., van der Zeijst B.A.M.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-82.
      Strain: 129108.

    Entry informationi

    Entry nameiSYE2_CAMJE
    AccessioniPrimary (citable) accession number: O52914
    Secondary accession number(s): Q0P8W9, Q9PN11
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3