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Protein

Beta-galactosidase

Gene

bgaM

Organism
Bacillus megaterium (strain DSM 319)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei481 – 4811Proton donorBy similarity
Active sitei547 – 5471NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciBMEG592022:GIVX-2126-MONOMER.
BRENDAi3.2.1.23. 656.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:bgaM
Ordered Locus Names:BMD_2126
OrganismiBacillus megaterium (strain DSM 319)
Taxonomic identifieri592022 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000002365 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10341034Beta-galactosidasePRO_0000057659Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO52847.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

HOGENOMiHOG000252444.
KOiK01190.
OMAiYLWCNGR.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52847-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKTGKKFHY TAPANGYPEW NNNPEIFQLN RSKAHALLMP YQTVEEALKN
60 70 80 90 100
DRKSSVYYQS LNGSWYFHFA ENADGRVKNF FAPEFSYEKW DSISVPSHWQ
110 120 130 140 150
LQGYDYPQYT NVTYPWVENE ELEPPFAPTK YNPVGQYVRT FTPKSEWKDQ
160 170 180 190 200
PVYISFQGVE SAFYVWINGE FVGYSEDSFT PAEFDITSYL QEGENTIAVE
210 220 230 240 250
VYRWSDASWL EDQDFWRMSG IFRDVYLYST PQVHIYDFSV RSSLDNNYED
260 270 280 290 300
GELSVSADIL NYFEHDTQDL TFEVMLYDAN AQEVLQAPLQ TNLSVSDQRT
310 320 330 340 350
VSLRTHIKSP AKWSAESPNL YTLVLSLKNA AGSIIETESC KVGFRTFEIK
360 370 380 390 400
NGLMTINGKR IVLRGVNRHE FDSVKGRAGI TREDMIHDIL LMKQHNINAV
410 420 430 440 450
RTSHYPNDSV WYELCNEYGL YVIDETNLET HGTWTYLQEG EQKAVPGSKP
460 470 480 490 500
EWKENVLDRC RSMYERDKNH PSIIIWSLGN ESFGGENFQH MYTFFKEKDS
510 520 530 540 550
TRLVHYEGIF HHRDYDASDI ESTMYVKPAD VERYALMNPK KPYILCEYSH
560 570 580 590 600
AMGNSCGNLY KYWELFDQYP ILQGGFIWDW KDQALQATAE DGTSYLAYGG
610 620 630 640 650
DFGDTPNDGN FCGNGLIFAD GTASPKIAEV KKCYQPVKWT AVDPAKGKFA
660 670 680 690 700
VQNKHLFTNL NAYDFVWTVE KNGELVEKHA SLLNVAPDGT DELTLSYPLY
710 720 730 740 750
EQENETDEFV LTLSLRLSKD TAWASAGYEV AYEQFVLPAK AAMPSVKAAH
760 770 780 790 800
PALTVDQNEQ TLTVTGTNFT AIFDKRKGQF ISYNYERTEL LASGFRPNFW
810 820 830 840 850
RAVTDNDLGN KLHERCQTWR QASLEQHVKK VTVQPQVDFV IISVELALDN
860 870 880 890 900
SLASCYVTYT LYNDGEMKIE QSLAPSETMP EIPEIGMLFT MNAAFDSLTW
910 920 930 940 950
YGRGPHENYW DRKTGAKLAL HKGSVKEQVT PYLRPQECGN KTDVRWATIT
960 970 980 990 1000
NDQGRGFLIK GLPTVELNAL PYSPFELEAY DHFYKLPASD SVTVRVNYKQ
1010 1020 1030
MGVGGDDSWQ AKTHPNYTLY ANRSYTNTFT LKPL
Length:1,034
Mass (Da):118,674
Last modified:June 1, 1998 - v1
Checksum:i38644C9A649415E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000733 Genomic DNA. Translation: CAA04267.1.
CP001982 Genomic DNA. Translation: ADF38979.1.
PIRiT30574.
RefSeqiWP_013082993.1. NC_014103.1.

Genome annotation databases

EnsemblBacteriaiADF38979; ADF38979; BMD_2126.
GeneIDi9117517.
KEGGibmd:BMD_2126.
PATRICi37254301. VBIBacMeg104484_2076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000733 Genomic DNA. Translation: CAA04267.1.
CP001982 Genomic DNA. Translation: ADF38979.1.
PIRiT30574.
RefSeqiWP_013082993.1. NC_014103.1.

3D structure databases

ProteinModelPortaliO52847.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADF38979; ADF38979; BMD_2126.
GeneIDi9117517.
KEGGibmd:BMD_2126.
PATRICi37254301. VBIBacMeg104484_2076.

Phylogenomic databases

HOGENOMiHOG000252444.
KOiK01190.
OMAiYLWCNGR.

Enzyme and pathway databases

BioCyciBMEG592022:GIVX-2126-MONOMER.
BRENDAi3.2.1.23. 656.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Strey J.
    Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure."
    Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S.
    Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 319.

Entry informationi

Entry nameiBGAL_BACMD
AccessioniPrimary (citable) accession number: O52847
Secondary accession number(s): D5DFH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: July 22, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.