O52847 (BGAL_BACMD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase
Short name=Beta-gal
EC=3.2.1.23

Alternative name(s):
Lactase

Gene names

Name:	bgaM
Ordered Locus Names:	BMD_2126

Organism

Bacillus megaterium (strain DSM 319) [Complete proteome] [HAMAP]

Taxonomic identifier

592022 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	1034 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1034	1034	Beta-galactosidase		PRO_0000057659
Sites
	Active site	481	1	Proton donor By similarity
	Active site	547	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	O52847 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 38644C9A649415E9	FASTA	1,034	118,674
	10 20 30 40 50 60 MLKTGKKFHY TAPANGYPEW NNNPEIFQLN RSKAHALLMP YQTVEEALKN DRKSSVYYQS 70 80 90 100 110 120 LNGSWYFHFA ENADGRVKNF FAPEFSYEKW DSISVPSHWQ LQGYDYPQYT NVTYPWVENE 130 140 150 160 170 180 ELEPPFAPTK YNPVGQYVRT FTPKSEWKDQ PVYISFQGVE SAFYVWINGE FVGYSEDSFT 190 200 210 220 230 240 PAEFDITSYL QEGENTIAVE VYRWSDASWL EDQDFWRMSG IFRDVYLYST PQVHIYDFSV 250 260 270 280 290 300 RSSLDNNYED GELSVSADIL NYFEHDTQDL TFEVMLYDAN AQEVLQAPLQ TNLSVSDQRT 310 320 330 340 350 360 VSLRTHIKSP AKWSAESPNL YTLVLSLKNA AGSIIETESC KVGFRTFEIK NGLMTINGKR 370 380 390 400 410 420 IVLRGVNRHE FDSVKGRAGI TREDMIHDIL LMKQHNINAV RTSHYPNDSV WYELCNEYGL 430 440 450 460 470 480 YVIDETNLET HGTWTYLQEG EQKAVPGSKP EWKENVLDRC RSMYERDKNH PSIIIWSLGN 490 500 510 520 530 540 ESFGGENFQH MYTFFKEKDS TRLVHYEGIF HHRDYDASDI ESTMYVKPAD VERYALMNPK 550 560 570 580 590 600 KPYILCEYSH AMGNSCGNLY KYWELFDQYP ILQGGFIWDW KDQALQATAE DGTSYLAYGG 610 620 630 640 650 660 DFGDTPNDGN FCGNGLIFAD GTASPKIAEV KKCYQPVKWT AVDPAKGKFA VQNKHLFTNL 670 680 690 700 710 720 NAYDFVWTVE KNGELVEKHA SLLNVAPDGT DELTLSYPLY EQENETDEFV LTLSLRLSKD 730 740 750 760 770 780 TAWASAGYEV AYEQFVLPAK AAMPSVKAAH PALTVDQNEQ TLTVTGTNFT AIFDKRKGQF 790 800 810 820 830 840 ISYNYERTEL LASGFRPNFW RAVTDNDLGN KLHERCQTWR QASLEQHVKK VTVQPQVDFV 850 860 870 880 890 900 IISVELALDN SLASCYVTYT LYNDGEMKIE QSLAPSETMP EIPEIGMLFT MNAAFDSLTW 910 920 930 940 950 960 YGRGPHENYW DRKTGAKLAL HKGSVKEQVT PYLRPQECGN KTDVRWATIT NDQGRGFLIK 970 980 990 1000 1010 1020 GLPTVELNAL PYSPFELEAY DHFYKLPASD SVTVRVNYKQ MGVGGDDSWQ AKTHPNYTLY 1030 ANRSYTNTFT LKPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Strey J. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure." Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S. Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 319.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ000733 Genomic DNA. Translation: CAA04267.1. CP001982 Genomic DNA. Translation: ADF38979.1.
PIR	T30574.
RefSeq	YP_003597329.1. NC_014103.1.
3D structure databases
ProteinModelPortal	O52847.
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000420488; EBBACP00000416801; EBBACG00000419804.
GeneID	9117517.
GenomeReviews	Gene locus BMD_2126 in contig CP001982_GR.
KEGG	bmd:BMD_2126.
PATRIC	37254301. VBIBacMeg104484_2076.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000006230.
ProtClustDB	CLSK2551175.
Family and domain databases
InterPro	IPR004199. B-gal_small/dom_5. IPR008979. Galactose-bd-like. IPR011013. Glyco_hydro-type_carb-bd. IPR014718. Glyco_hydro-type_carb-bd_sub. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR023232. Glyco_hydro_2_AS. IPR023230. Glyco_hydro_2_CS. IPR006102. Glyco_hydro_2_Ig-like. IPR006104. Glyco_hydro_2_N. IPR006103. Glyco_hydro_2_TIM. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits. G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K01190.
Pfam	PF02929. Bgal_small_N. 1 hit. PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
SMART	SM01038. Bgal_small_N. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF74650. Gal_mut_like. 1 hit. SSF49303. Glyco_hydro_2Ig. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_BACMD

Accession

Primary (citable) accession number: O52847
Secondary accession number(s): D5DFH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program