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O52847 (BGAL_BACMD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:bgaM
Ordered Locus Names:BMD_2126
OrganismBacillus megaterium (strain DSM 319) [Complete proteome] [HAMAP]
Taxonomic identifier592022 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length1034 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10341034Beta-galactosidase
PRO_0000057659

Sites

Active site4811Proton donor By similarity
Active site5471Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
O52847 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 38644C9A649415E9

FASTA1,034118,674
        10         20         30         40         50         60 
MLKTGKKFHY TAPANGYPEW NNNPEIFQLN RSKAHALLMP YQTVEEALKN DRKSSVYYQS 

        70         80         90        100        110        120 
LNGSWYFHFA ENADGRVKNF FAPEFSYEKW DSISVPSHWQ LQGYDYPQYT NVTYPWVENE 

       130        140        150        160        170        180 
ELEPPFAPTK YNPVGQYVRT FTPKSEWKDQ PVYISFQGVE SAFYVWINGE FVGYSEDSFT 

       190        200        210        220        230        240 
PAEFDITSYL QEGENTIAVE VYRWSDASWL EDQDFWRMSG IFRDVYLYST PQVHIYDFSV 

       250        260        270        280        290        300 
RSSLDNNYED GELSVSADIL NYFEHDTQDL TFEVMLYDAN AQEVLQAPLQ TNLSVSDQRT 

       310        320        330        340        350        360 
VSLRTHIKSP AKWSAESPNL YTLVLSLKNA AGSIIETESC KVGFRTFEIK NGLMTINGKR 

       370        380        390        400        410        420 
IVLRGVNRHE FDSVKGRAGI TREDMIHDIL LMKQHNINAV RTSHYPNDSV WYELCNEYGL 

       430        440        450        460        470        480 
YVIDETNLET HGTWTYLQEG EQKAVPGSKP EWKENVLDRC RSMYERDKNH PSIIIWSLGN 

       490        500        510        520        530        540 
ESFGGENFQH MYTFFKEKDS TRLVHYEGIF HHRDYDASDI ESTMYVKPAD VERYALMNPK 

       550        560        570        580        590        600 
KPYILCEYSH AMGNSCGNLY KYWELFDQYP ILQGGFIWDW KDQALQATAE DGTSYLAYGG 

       610        620        630        640        650        660 
DFGDTPNDGN FCGNGLIFAD GTASPKIAEV KKCYQPVKWT AVDPAKGKFA VQNKHLFTNL 

       670        680        690        700        710        720 
NAYDFVWTVE KNGELVEKHA SLLNVAPDGT DELTLSYPLY EQENETDEFV LTLSLRLSKD 

       730        740        750        760        770        780 
TAWASAGYEV AYEQFVLPAK AAMPSVKAAH PALTVDQNEQ TLTVTGTNFT AIFDKRKGQF 

       790        800        810        820        830        840 
ISYNYERTEL LASGFRPNFW RAVTDNDLGN KLHERCQTWR QASLEQHVKK VTVQPQVDFV 

       850        860        870        880        890        900 
IISVELALDN SLASCYVTYT LYNDGEMKIE QSLAPSETMP EIPEIGMLFT MNAAFDSLTW 

       910        920        930        940        950        960 
YGRGPHENYW DRKTGAKLAL HKGSVKEQVT PYLRPQECGN KTDVRWATIT NDQGRGFLIK 

       970        980        990       1000       1010       1020 
GLPTVELNAL PYSPFELEAY DHFYKLPASD SVTVRVNYKQ MGVGGDDSWQ AKTHPNYTLY 

      1030 
ANRSYTNTFT LKPL 

« Hide

References

« Hide 'large scale' references
[1]Strey J.
Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure."
Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S.
Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 319.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ000733 Genomic DNA. Translation: CAA04267.1.
CP001982 Genomic DNA. Translation: ADF38979.1.
PIRT30574.
RefSeqYP_003597329.1. NC_014103.1.

3D structure databases

ProteinModelPortalO52847.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADF38979; ADF38979; BMD_2126.
GeneID9117517.
KEGGbmd:BMD_2126.
PATRIC37254301. VBIBacMeg104484_2076.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000252444.
KOK01190.
OMARTIERDK.

Enzyme and pathway databases

BioCycBMEG592022:GIVX-2126-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_BACMD
AccessionPrimary (citable) accession number: O52847
Secondary accession number(s): D5DFH9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries