Reviewed, UniProtKB/Swiss-Prot O52847 (BGAL_BACME)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase
      Short name=Beta-gal
    EC=3.2.1.23
Alternative name(s):
    Lactase

Gene names

Name:

bgaM

Organism

Bacillus megaterium

Taxonomic identifier

1404 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	1034 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1034	1034	Beta-galactosidase		PRO_0000057659
Sites
	Active site	481	1	Proton donor By similarity
	Active site	547	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	O52847-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 38644C9A649415E9	FASTA	1,034	118,674
	10 20 30 40 50 60 MLKTGKKFHY TAPANGYPEW NNNPEIFQLN RSKAHALLMP YQTVEEALKN DRKSSVYYQS 70 80 90 100 110 120 LNGSWYFHFA ENADGRVKNF FAPEFSYEKW DSISVPSHWQ LQGYDYPQYT NVTYPWVENE 130 140 150 160 170 180 ELEPPFAPTK YNPVGQYVRT FTPKSEWKDQ PVYISFQGVE SAFYVWINGE FVGYSEDSFT 190 200 210 220 230 240 PAEFDITSYL QEGENTIAVE VYRWSDASWL EDQDFWRMSG IFRDVYLYST PQVHIYDFSV 250 260 270 280 290 300 RSSLDNNYED GELSVSADIL NYFEHDTQDL TFEVMLYDAN AQEVLQAPLQ TNLSVSDQRT 310 320 330 340 350 360 VSLRTHIKSP AKWSAESPNL YTLVLSLKNA AGSIIETESC KVGFRTFEIK NGLMTINGKR 370 380 390 400 410 420 IVLRGVNRHE FDSVKGRAGI TREDMIHDIL LMKQHNINAV RTSHYPNDSV WYELCNEYGL 430 440 450 460 470 480 YVIDETNLET HGTWTYLQEG EQKAVPGSKP EWKENVLDRC RSMYERDKNH PSIIIWSLGN 490 500 510 520 530 540 ESFGGENFQH MYTFFKEKDS TRLVHYEGIF HHRDYDASDI ESTMYVKPAD VERYALMNPK 550 560 570 580 590 600 KPYILCEYSH AMGNSCGNLY KYWELFDQYP ILQGGFIWDW KDQALQATAE DGTSYLAYGG 610 620 630 640 650 660 DFGDTPNDGN FCGNGLIFAD GTASPKIAEV KKCYQPVKWT AVDPAKGKFA VQNKHLFTNL 670 680 690 700 710 720 NAYDFVWTVE KNGELVEKHA SLLNVAPDGT DELTLSYPLY EQENETDEFV LTLSLRLSKD 730 740 750 760 770 780 TAWASAGYEV AYEQFVLPAK AAMPSVKAAH PALTVDQNEQ TLTVTGTNFT AIFDKRKGQF 790 800 810 820 830 840 ISYNYERTEL LASGFRPNFW RAVTDNDLGN KLHERCQTWR QASLEQHVKK VTVQPQVDFV 850 860 870 880 890 900 IISVELALDN SLASCYVTYT LYNDGEMKIE QSLAPSETMP EIPEIGMLFT MNAAFDSLTW 910 920 930 940 950 960 YGRGPHENYW DRKTGAKLAL HKGSVKEQVT PYLRPQECGN KTDVRWATIT NDQGRGFLIK 970 980 990 1000 1010 1020 GLPTVELNAL PYSPFELEAY DHFYKLPASD SVTVRVNYKQ MGVGGDDSWQ AKTHPNYTLY 1030 ANRSYTNTFT LKPL

References

[1]	Strey J. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 319.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ000733 Genomic DNA. Translation: CAA04267.1.
PIR	T30574.
3D structure databases
HSSP	HSSP built from PDB template 1F49 based on UniProtKB P00722.
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Enzyme and pathway databases
BRENDA	3.2.1.23. 325.
Family and domain databases
InterPro	IPR014718. Glyco_hydro-type_carb-bd_sub. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006104. Glyco_hydro_2_carb-bd. IPR006102. Glyco_hydro_2_Ig-like. IPR006103. Glyco_hydro_2_TIM. IPR004199. Glyco_hydro_42_D5. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits. G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02929. Bgal_small_N. 1 hit. PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_BACME

Accession

Primary (citable) accession number: O52847

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)