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O52703 (T2A1_ACEPA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme ApaLI

Short name=R.ApaLI
EC=3.1.21.4
Alternative name(s):
Endonuclease ApaLI
Type II restriction enzyme ApaLI
Gene names
Name:apaLIR
OrganismAcetobacter pasteurianus (Acetobacter turbidans)
Taxonomic identifier438 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeAcetobacter

Protein attributes

Sequence length375 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GTGCAC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 375375Type-2 restriction enzyme ApaLI
PRO_0000077275

Sequences

Sequence LengthMass (Da)Tools
O52703 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 9E4FFBB05279A94C

FASTA37541,730
        10         20         30         40         50         60 
MTTRQRLSAE RSQQLTRLLT ITKTANMRAL MEASELAKVI ALVAVDIGKS DEMARAFPVL 

        70         80         90        100        110        120 
WPKISPQQEY YATAVDWFTN PDETVTSFDV VDMLDAGTSL DQDFMTYLKC LTELHKRRRK 

       130        140        150        160        170        180 
YGLILQRQPL PTMVQVSPRA LMEYGPDFPP EALASWLTWR KFFYDLDNRS AQETGYLFEP 

       190        200        210        220        230        240 
ILAAAIGGEA KSARERVVRR TDDPTKGRQV DCWKVLPDGT PLAYELKLRV TIAASGQGRF 

       250        260        270        280        290        300 
GEELSFARDC SSSGAKPILV VLDPTENDKL TGLQAAYREV GGAAYVGDAA WAHLEDEAGA 

       310        320        330        340        350        360 
TMASFIERYV RVPVASVSSF ERVIEGDATK RSLILQDLQA RLDGNELTIS LGGHQRLVER 

       370 
HEDQSLAADG DDDSE 

« Hide

References

[1]"Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 12875.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF044847 Genomic DNA. Translation: AAC97181.1.

3D structure databases

ProteinModelPortalO52703.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE85. ApaLI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019036. Restrct_endonuc_II_ApaLI.
[Graphical view]
PfamPF09499. RE_ApaLI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2A1_ACEPA
AccessionPrimary (citable) accession number: O52703
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries