SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O52703

- T2A1_ACEPA

UniProt

O52703 - T2A1_ACEPA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Type-2 restriction enzyme ApaLI
Gene
apaLIR
Organism
Acetobacter pasteurianus (Acetobacter turbidans)
Status
Reviewed - Annotation score: 2 out of 5 - Protein predictedi

Functioni

Recognizes the double-stranded sequence GTGCAC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi85. ApaLI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme ApaLI (EC:3.1.21.4)
Short name:
R.ApaLI
Alternative name(s):
Endonuclease ApaLI
Type II restriction enzyme ApaLI
Gene namesi
Name:apaLIR
OrganismiAcetobacter pasteurianus (Acetobacter turbidans)
Taxonomic identifieri438 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeAcetobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Type-2 restriction enzyme ApaLI
PRO_0000077275Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO52703.

Family & Domainsi

Family and domain databases

InterProiIPR019036. Restrct_endonuc_II_ApaLI.
[Graphical view]
PfamiPF09499. RE_ApaLI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52703-1 [UniParc]FASTAAdd to Basket

« Hide

MTTRQRLSAE RSQQLTRLLT ITKTANMRAL MEASELAKVI ALVAVDIGKS    50
DEMARAFPVL WPKISPQQEY YATAVDWFTN PDETVTSFDV VDMLDAGTSL 100
DQDFMTYLKC LTELHKRRRK YGLILQRQPL PTMVQVSPRA LMEYGPDFPP 150
EALASWLTWR KFFYDLDNRS AQETGYLFEP ILAAAIGGEA KSARERVVRR 200
TDDPTKGRQV DCWKVLPDGT PLAYELKLRV TIAASGQGRF GEELSFARDC 250
SSSGAKPILV VLDPTENDKL TGLQAAYREV GGAAYVGDAA WAHLEDEAGA 300
TMASFIERYV RVPVASVSSF ERVIEGDATK RSLILQDLQA RLDGNELTIS 350
LGGHQRLVER HEDQSLAADG DDDSE 375
Length:375
Mass (Da):41,730
Last modified:June 1, 1998 - v1
Checksum:i9E4FFBB05279A94C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF044847 Genomic DNA. Translation: AAC97181.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF044847 Genomic DNA. Translation: AAC97181.1 .

3D structure databases

ProteinModelPortali O52703.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 85. ApaLI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR019036. Restrct_endonuc_II_ApaLI.
[Graphical view ]
Pfami PF09499. RE_ApaLI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
    Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
    Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 12875.

Entry informationi

Entry nameiT2A1_ACEPA
AccessioniPrimary (citable) accession number: O52703
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi