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O52703

- T2A1_ACEPA

UniProt

O52703 - T2A1_ACEPA

Protein

Type-2 restriction enzyme ApaLI

Gene

apaLIR

Organism
Acetobacter pasteurianus (Acetobacter turbidans)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 41 (16 Apr 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GTGCAC and cleaves after G-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi85. ApaLI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme ApaLI (EC:3.1.21.4)
    Short name:
    R.ApaLI
    Alternative name(s):
    Endonuclease ApaLI
    Type II restriction enzyme ApaLI
    Gene namesi
    Name:apaLIR
    OrganismiAcetobacter pasteurianus (Acetobacter turbidans)
    Taxonomic identifieri438 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeAcetobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 375375Type-2 restriction enzyme ApaLIPRO_0000077275Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliO52703.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Family and domain databases

    InterProiIPR019036. Restrct_endonuc_II_ApaLI.
    [Graphical view]
    PfamiPF09499. RE_ApaLI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O52703-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTRQRLSAE RSQQLTRLLT ITKTANMRAL MEASELAKVI ALVAVDIGKS    50
    DEMARAFPVL WPKISPQQEY YATAVDWFTN PDETVTSFDV VDMLDAGTSL 100
    DQDFMTYLKC LTELHKRRRK YGLILQRQPL PTMVQVSPRA LMEYGPDFPP 150
    EALASWLTWR KFFYDLDNRS AQETGYLFEP ILAAAIGGEA KSARERVVRR 200
    TDDPTKGRQV DCWKVLPDGT PLAYELKLRV TIAASGQGRF GEELSFARDC 250
    SSSGAKPILV VLDPTENDKL TGLQAAYREV GGAAYVGDAA WAHLEDEAGA 300
    TMASFIERYV RVPVASVSSF ERVIEGDATK RSLILQDLQA RLDGNELTIS 350
    LGGHQRLVER HEDQSLAADG DDDSE 375
    Length:375
    Mass (Da):41,730
    Last modified:June 1, 1998 - v1
    Checksum:i9E4FFBB05279A94C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF044847 Genomic DNA. Translation: AAC97181.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF044847 Genomic DNA. Translation: AAC97181.1 .

    3D structure databases

    ProteinModelPortali O52703.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 85. ApaLI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019036. Restrct_endonuc_II_ApaLI.
    [Graphical view ]
    Pfami PF09499. RE_ApaLI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
      Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
      Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 12875.

    Entry informationi

    Entry nameiT2A1_ACEPA
    AccessioniPrimary (citable) accession number: O52703
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: June 1, 1998
    Last modified: April 16, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3