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O52703

- T2A1_ACEPA

UniProt

O52703 - T2A1_ACEPA

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Protein

Type-2 restriction enzyme ApaLI

Gene

apaLIR

Organism
Acetobacter pasteurianus (Acetobacter turbidans)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi

Functioni

Recognizes the double-stranded sequence GTGCAC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi85. ApaLI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme ApaLI (EC:3.1.21.4)
Short name:
R.ApaLI
Alternative name(s):
Endonuclease ApaLI
Type II restriction enzyme ApaLI
Gene namesi
Name:apaLIR
OrganismiAcetobacter pasteurianus (Acetobacter turbidans)
Taxonomic identifieri438 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeAcetobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Type-2 restriction enzyme ApaLIPRO_0000077275Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO52703.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Family and domain databases

InterProiIPR019036. Restrct_endonuc_II_ApaLI.
[Graphical view]
PfamiPF09499. RE_ApaLI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52703-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTRQRLSAE RSQQLTRLLT ITKTANMRAL MEASELAKVI ALVAVDIGKS
60 70 80 90 100
DEMARAFPVL WPKISPQQEY YATAVDWFTN PDETVTSFDV VDMLDAGTSL
110 120 130 140 150
DQDFMTYLKC LTELHKRRRK YGLILQRQPL PTMVQVSPRA LMEYGPDFPP
160 170 180 190 200
EALASWLTWR KFFYDLDNRS AQETGYLFEP ILAAAIGGEA KSARERVVRR
210 220 230 240 250
TDDPTKGRQV DCWKVLPDGT PLAYELKLRV TIAASGQGRF GEELSFARDC
260 270 280 290 300
SSSGAKPILV VLDPTENDKL TGLQAAYREV GGAAYVGDAA WAHLEDEAGA
310 320 330 340 350
TMASFIERYV RVPVASVSSF ERVIEGDATK RSLILQDLQA RLDGNELTIS
360 370
LGGHQRLVER HEDQSLAADG DDDSE
Length:375
Mass (Da):41,730
Last modified:June 1, 1998 - v1
Checksum:i9E4FFBB05279A94C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044847 Genomic DNA. Translation: AAC97181.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044847 Genomic DNA. Translation: AAC97181.1 .

3D structure databases

ProteinModelPortali O52703.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 85. ApaLI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR019036. Restrct_endonuc_II_ApaLI.
[Graphical view ]
Pfami PF09499. RE_ApaLI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
    Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
    Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 12875.

Entry informationi

Entry nameiT2A1_ACEPA
AccessioniPrimary (citable) accession number: O52703
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3