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O52702

- MTA1_ACEPA

UniProt

O52702 - MTA1_ACEPA

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Protein

Modification methylase ApaLI

Gene

apaLIM

Organism
Acetobacter pasteurianus (Acetobacter turbidans)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GTGCAC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the ApaLI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei81 – 811PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3281. M.ApaLI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase ApaLI (EC:2.1.1.37)
Short name:
M.ApaLI
Alternative name(s):
Cytosine-specific methyltransferase ApaLI
Gene namesi
Name:apaLIM
OrganismiAcetobacter pasteurianus (Acetobacter turbidans)
Taxonomic identifieri438 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeAcetobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Modification methylase ApaLIPRO_0000087857Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO52702.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 383379SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52702-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKDEVVVSL FAGAGGFSSG FSQAGLKPLF GAEINADACQ TYQENVGSPC
60 70 80 90 100
HQLDLSTVDP SHIEMLTGGK RPFVVIGGPP CQGFSTAGPR NFADPRNLLI
110 120 130 140 150
FNYLNIVERL SPRWLIFENV EGLLTSGGGR DLARLVREFV DMGYSVRLQK
160 170 180 190 200
VNLAAYGVPQ TRKRVLIIGN RLGIDFQFPE ELYSFDSGKA KKASGKPLAP
210 220 230 240 250
SLAEAVAGLG PAASDKDALV PYASSEPVNA FDARMRAGNR VEVVTHHVRV
260 270 280 290 300
EAAERMQVEL LKPGQTMKDL PPELWHESYR RRANRRVSDG TPTEKRGGAP
310 320 330 340 350
SGIKRLHGNL QSLTITGPAA REFIHPTEHR PLTIRECARI QTFPDKYRWV
360 370 380 390 400
GNNASVIQQI GNAVPPLAAE RLAKHLRDID GSFGADTRPA GAMSAKLLGF
410 420
VLTEALGMSP ALKSTEALLA EMHQGGFVF
Length:429
Mass (Da):46,547
Last modified:June 1, 1998 - v1
Checksum:iE011C7D15B33F5F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044847 Genomic DNA. Translation: AAC97180.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044847 Genomic DNA. Translation: AAC97180.1 .

3D structure databases

ProteinModelPortali O52702.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3281. M.ApaLI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
    Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
    Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 12875.

Entry informationi

Entry nameiMTA1_ACEPA
AccessioniPrimary (citable) accession number: O52702
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3