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O52702

- MTA1_ACEPA

UniProt

O52702 - MTA1_ACEPA

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Protein
Modification methylase ApaLI
Gene
apaLIM
Organism
Acetobacter pasteurianus (Acetobacter turbidans)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GTGCAC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the ApaLI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei81 – 811 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3281. M.ApaLI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase ApaLI (EC:2.1.1.37)
Short name:
M.ApaLI
Alternative name(s):
Cytosine-specific methyltransferase ApaLI
Gene namesi
Name:apaLIM
OrganismiAcetobacter pasteurianus (Acetobacter turbidans)
Taxonomic identifieri438 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeAcetobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Modification methylase ApaLI
PRO_0000087857Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO52702.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 383379SAM-dependent MTase C5-type
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52702-1 [UniParc]FASTAAdd to Basket

« Hide

MNKDEVVVSL FAGAGGFSSG FSQAGLKPLF GAEINADACQ TYQENVGSPC    50
HQLDLSTVDP SHIEMLTGGK RPFVVIGGPP CQGFSTAGPR NFADPRNLLI 100
FNYLNIVERL SPRWLIFENV EGLLTSGGGR DLARLVREFV DMGYSVRLQK 150
VNLAAYGVPQ TRKRVLIIGN RLGIDFQFPE ELYSFDSGKA KKASGKPLAP 200
SLAEAVAGLG PAASDKDALV PYASSEPVNA FDARMRAGNR VEVVTHHVRV 250
EAAERMQVEL LKPGQTMKDL PPELWHESYR RRANRRVSDG TPTEKRGGAP 300
SGIKRLHGNL QSLTITGPAA REFIHPTEHR PLTIRECARI QTFPDKYRWV 350
GNNASVIQQI GNAVPPLAAE RLAKHLRDID GSFGADTRPA GAMSAKLLGF 400
VLTEALGMSP ALKSTEALLA EMHQGGFVF 429
Length:429
Mass (Da):46,547
Last modified:June 1, 1998 - v1
Checksum:iE011C7D15B33F5F3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF044847 Genomic DNA. Translation: AAC97180.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF044847 Genomic DNA. Translation: AAC97180.1 .

3D structure databases

ProteinModelPortali O52702.
ModBasei Search...

Protein family/group databases

REBASEi 3281. M.ApaLI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
    Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
    Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 12875.

Entry informationi

Entry nameiMTA1_ACEPA
AccessioniPrimary (citable) accession number: O52702
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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