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O52702 (MTA1_ACEPA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase ApaLI

Short name=M.ApaLI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase ApaLI
Gene names
Name:apaLIM
OrganismAcetobacter pasteurianus (Acetobacter turbidans)
Taxonomic identifier438 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeAcetobacter

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GTGCAC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the ApaLI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429Modification methylase ApaLI
PRO_0000087857

Regions

Domain5 – 383379SAM-dependent MTase C5-type

Sites

Active site811 By similarity

Sequences

Sequence LengthMass (Da)Tools
O52702 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: E011C7D15B33F5F3

FASTA42946,547
        10         20         30         40         50         60 
MNKDEVVVSL FAGAGGFSSG FSQAGLKPLF GAEINADACQ TYQENVGSPC HQLDLSTVDP 

        70         80         90        100        110        120 
SHIEMLTGGK RPFVVIGGPP CQGFSTAGPR NFADPRNLLI FNYLNIVERL SPRWLIFENV 

       130        140        150        160        170        180 
EGLLTSGGGR DLARLVREFV DMGYSVRLQK VNLAAYGVPQ TRKRVLIIGN RLGIDFQFPE 

       190        200        210        220        230        240 
ELYSFDSGKA KKASGKPLAP SLAEAVAGLG PAASDKDALV PYASSEPVNA FDARMRAGNR 

       250        260        270        280        290        300 
VEVVTHHVRV EAAERMQVEL LKPGQTMKDL PPELWHESYR RRANRRVSDG TPTEKRGGAP 

       310        320        330        340        350        360 
SGIKRLHGNL QSLTITGPAA REFIHPTEHR PLTIRECARI QTFPDKYRWV GNNASVIQQI 

       370        380        390        400        410        420 
GNAVPPLAAE RLAKHLRDID GSFGADTRPA GAMSAKLLGF VLTEALGMSP ALKSTEALLA 


EMHQGGFVF 

« Hide

References

[1]"Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 12875.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF044847 Genomic DNA. Translation: AAC97180.1.

3D structure databases

ProteinModelPortalO52702.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3281. M.ApaLI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTA1_ACEPA
AccessionPrimary (citable) accession number: O52702
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries