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Protein

Modification methylase ScaI

Gene

scaIM

Organism
Streptomyces caespitosus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence AGTACT, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the ScaI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.113. 5987.

Protein family/group databases

REBASEi3498. M.ScaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase ScaI (EC:2.1.1.113)
Short name:
M.ScaI
Alternative name(s):
N-4 cytosine-specific methyltransferase ScaI
Gene namesi
Name:scaIM
OrganismiStreptomyces caespitosus
Taxonomic identifieri53502 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Modification methylase ScaIPRO_0000087933Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO52692.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR017985. MeTrfase_CN4_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00093. N4_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52692-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGRDFGYVI QSSAALWNRL STFSQRGKAL DTRLADIKKA LGKPYYETSD
60 70 80 90 100
VLLYHGDSLE LLKSMPQQIF DLTVTSPPYN IGKEYEGVLS IEEYISWCET
110 120 130 140 150
WMSRVHRATS AGGAFWLNVG YVPVPNQGKA VPIPYLLWDK SPFYMIQEVV
160 170 180 190 200
WNYGAGVASR KSFSPRNEKF LWYVRDPLNY YFDLDSVRDP NVKYPNQKKN
210 220 230 240 250
GKLKCNPLGK NPTDVWQFPK VTSGAKRSSV ERTAHPAQFP SAVIERVIKA
260 270 280 290 300
CSPSDGVILD PFLGSGTTSL TARKQGRCSV GIEIREDYLD IAVGRLEAEA

QSLF
Length:304
Mass (Da):34,187
Last modified:June 1, 1998 - v1
Checksum:iF589CF18B62C3634
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044681 Genomic DNA. Translation: AAC97178.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044681 Genomic DNA. Translation: AAC97178.1.

3D structure databases

ProteinModelPortaliO52692.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3498. M.ScaI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.1.1.113. 5987.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR017985. MeTrfase_CN4_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00093. N4_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTS1_STRCS
AccessioniPrimary (citable) accession number: O52692
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: June 24, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.