O52692 (MTS1_STRCS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase ScaI
Short name=M.ScaI
EC=2.1.1.113

Alternative name(s):
N-4 cytosine-specific methyltransferase ScaI

Gene names

Name:

scaIM

Organism

Streptomyces caespitosus

Taxonomic identifier

53502 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence AGTACT, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the ScaI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Modification methylase ScaI		PRO_0000087933

Sequences

	Sequence		Length	Mass (Da)
	O52692 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: F589CF18B62C3634	FASTA	304	34,187
	10 20 30 40 50 60 MSGRDFGYVI QSSAALWNRL STFSQRGKAL DTRLADIKKA LGKPYYETSD VLLYHGDSLE 70 80 90 100 110 120 LLKSMPQQIF DLTVTSPPYN IGKEYEGVLS IEEYISWCET WMSRVHRATS AGGAFWLNVG 130 140 150 160 170 180 YVPVPNQGKA VPIPYLLWDK SPFYMIQEVV WNYGAGVASR KSFSPRNEKF LWYVRDPLNY 190 200 210 220 230 240 YFDLDSVRDP NVKYPNQKKN GKLKCNPLGK NPTDVWQFPK VTSGAKRSSV ERTAHPAQFP 250 260 270 280 290 300 SAVIERVIKA CSPSDGVILD PFLGSGTTSL TARKQGRCSV GIEIREDYLD IAVGRLEAEA QSLF

References

[1]

"Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli." Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II Mol. Gen. Genet. 260:226-231(1998) [PubMed: 9862476] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF044681 Genomic DNA. Translation: AAC97178.1.
3D structure databases
ProteinModelPortal	O52692.
ModBase	Search...
Protein family/group databases
REBASE	3498. M.ScaI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.113. 5987.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR017985. MeTrfase_CN4_CS. IPR001091. RM_Methylase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTS1_STRCS

Accession

Primary (citable) accession number: O52692

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	June 1, 1998
Last modified:	September 21, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program