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O52691

- T2S1_STRCS

UniProt

O52691 - T2S1_STRCS

Protein

Type-2 restriction enzyme ScaI

Gene

scaIR

Organism
Streptomyces caespitosus
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 43 (16 Apr 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence AGTACT and cleaves after T-3.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1628. ScaI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme ScaI (EC:3.1.21.4)
    Short name:
    R.ScaI
    Alternative name(s):
    Endonuclease ScaI
    Type II restriction enzyme ScaI
    Gene namesi
    Name:scaIR
    OrganismiStreptomyces caespitosus
    Taxonomic identifieri53502 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 227227Type-2 restriction enzyme ScaIPRO_0000077367Add
    BLAST

    Interactioni

    Family & Domainsi

    Family and domain databases

    InterProiIPR019069. Restrct_endonuc_II_ScaI.
    [Graphical view]
    PfamiPF09569. RE_ScaI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O52691-1 [UniParc]FASTAAdd to Basket

    « Hide

    MINDQLPRWV REARVGTRTG GPAMRPKTSD SPYFGWDSED WPEVTRQLLS    50
    EQPLSGDTLV DAVLASWESI FESRLGSGFH IGTQIRPTPQ VMGFLLHALI 100
    PLELANGDPS WRADLNSSEK DLVYQPDHKY SIEMKTSSHK DQIFGNRSFG 150
    VENPGKGKKA KDGYYVAVNF EKWSDAPGRL PRIRTIRYGW LDHTDWVAQK 200
    SQTGQQSSLP AVVSNTQLLA IHTGGQR 227
    Length:227
    Mass (Da):25,464
    Last modified:June 1, 1998 - v1
    Checksum:iA4E48072756B6009
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF044681 Genomic DNA. Translation: AAC97177.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF044681 Genomic DNA. Translation: AAC97177.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi O52691.
    ChEMBLi CHEMBL5782.

    Protein family/group databases

    REBASEi 1628. ScaI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019069. Restrct_endonuc_II_ScaI.
    [Graphical view ]
    Pfami PF09569. RE_ScaI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
      Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
      Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiT2S1_STRCS
    AccessioniPrimary (citable) accession number: O52691
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: June 1, 1998
    Last modified: April 16, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3