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Protein

Type-2 restriction enzyme ScaI

Gene

scaIR

Organism
Streptomyces caespitosus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence AGTACT and cleaves after T-3.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1628. ScaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme ScaI (EC:3.1.21.4)
Short name:
R.ScaI
Alternative name(s):
Endonuclease ScaI
Type II restriction enzyme ScaI
Gene namesi
Name:scaIR
OrganismiStreptomyces caespitosus
Taxonomic identifieri53502 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5782.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773671 – 227Type-2 restriction enzyme ScaIAdd BLAST227

Interactioni

Chemistry databases

BindingDBiO52691.

Family & Domainsi

Family and domain databases

InterProiIPR019069. Restrct_endonuc_II_ScaI.
[Graphical view]
PfamiPF09569. RE_ScaI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52691-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINDQLPRWV REARVGTRTG GPAMRPKTSD SPYFGWDSED WPEVTRQLLS
60 70 80 90 100
EQPLSGDTLV DAVLASWESI FESRLGSGFH IGTQIRPTPQ VMGFLLHALI
110 120 130 140 150
PLELANGDPS WRADLNSSEK DLVYQPDHKY SIEMKTSSHK DQIFGNRSFG
160 170 180 190 200
VENPGKGKKA KDGYYVAVNF EKWSDAPGRL PRIRTIRYGW LDHTDWVAQK
210 220
SQTGQQSSLP AVVSNTQLLA IHTGGQR
Length:227
Mass (Da):25,464
Last modified:June 1, 1998 - v1
Checksum:iA4E48072756B6009
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044681 Genomic DNA. Translation: AAC97177.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044681 Genomic DNA. Translation: AAC97177.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiO52691.
ChEMBLiCHEMBL5782.

Protein family/group databases

REBASEi1628. ScaI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR019069. Restrct_endonuc_II_ScaI.
[Graphical view]
PfamiPF09569. RE_ScaI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiT2S1_STRCS
AccessioniPrimary (citable) accession number: O52691
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: June 24, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.