O52691 (T2S1_STRCS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme ScaI
Short name=R.ScaI
EC=3.1.21.4

Alternative name(s):
Endonuclease ScaI
Type II restriction enzyme ScaI

Gene names

Name:

scaIR

Organism

Streptomyces caespitosus

Taxonomic identifier

53502 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence AGTACT and cleaves after T-3.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Type-2 restriction enzyme ScaI		PRO_0000077367

Sequences

	Sequence		Length	Mass (Da)
	O52691 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: A4E48072756B6009	FASTA	227	25,464
	10 20 30 40 50 60 MINDQLPRWV REARVGTRTG GPAMRPKTSD SPYFGWDSED WPEVTRQLLS EQPLSGDTLV 70 80 90 100 110 120 DAVLASWESI FESRLGSGFH IGTQIRPTPQ VMGFLLHALI PLELANGDPS WRADLNSSEK 130 140 150 160 170 180 DLVYQPDHKY SIEMKTSSHK DQIFGNRSFG VENPGKGKKA KDGYYVAVNF EKWSDAPGRL 190 200 210 220 PRIRTIRYGW LDHTDWVAQK SQTGQQSSLP AVVSNTQLLA IHTGGQR

References

[1]

"Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli." Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II Mol. Gen. Genet. 260:226-231(1998) [PubMed: 9862476] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF044681 Genomic DNA. Translation: AAC97177.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1628. ScaI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019069. Restrct_endonuc_II_ScaI. [Graphical view]
Pfam	PF09569. RE_ScaI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2S1_STRCS

Accession

Primary (citable) accession number: O52691

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	June 1, 1998
Last modified:	May 31, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program