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O52691 (T2S1_STRCS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme ScaI

Short name=R.ScaI
EC=3.1.21.4
Alternative name(s):
Endonuclease ScaI
Type II restriction enzyme ScaI
Gene names
Name:scaIR
OrganismStreptomyces caespitosus
Taxonomic identifier53502 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence AGTACT and cleaves after T-3.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227Type-2 restriction enzyme ScaI
PRO_0000077367

Sequences

Sequence LengthMass (Da)Tools
O52691 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: A4E48072756B6009

FASTA22725,464
        10         20         30         40         50         60 
MINDQLPRWV REARVGTRTG GPAMRPKTSD SPYFGWDSED WPEVTRQLLS EQPLSGDTLV 

        70         80         90        100        110        120 
DAVLASWESI FESRLGSGFH IGTQIRPTPQ VMGFLLHALI PLELANGDPS WRADLNSSEK 

       130        140        150        160        170        180 
DLVYQPDHKY SIEMKTSSHK DQIFGNRSFG VENPGKGKKA KDGYYVAVNF EKWSDAPGRL 

       190        200        210        220 
PRIRTIRYGW LDHTDWVAQK SQTGQQSSLP AVVSNTQLLA IHTGGQR 

« Hide

References

[1]"Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF044681 Genomic DNA. Translation: AAC97177.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBO52691.
ChEMBLCHEMBL5782.

Protein family/group databases

REBASE1628. ScaI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019069. Restrct_endonuc_II_ScaI.
[Graphical view]
PfamPF09569. RE_ScaI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2S1_STRCS
AccessionPrimary (citable) accession number: O52691
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries