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O52691

- T2S1_STRCS

UniProt

O52691 - T2S1_STRCS

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Protein
Type-2 restriction enzyme ScaI
Gene
scaIR
Organism
Streptomyces caespitosus
Status
Reviewed - Annotation score: 2 out of 5 - Protein predictedi

Functioni

Recognizes the double-stranded sequence AGTACT and cleaves after T-3.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1628. ScaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme ScaI (EC:3.1.21.4)
Short name:
R.ScaI
Alternative name(s):
Endonuclease ScaI
Type II restriction enzyme ScaI
Gene namesi
Name:scaIR
OrganismiStreptomyces caespitosus
Taxonomic identifieri53502 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Type-2 restriction enzyme ScaI
PRO_0000077367Add
BLAST

Interactioni

Family & Domainsi

Family and domain databases

InterProiIPR019069. Restrct_endonuc_II_ScaI.
[Graphical view]
PfamiPF09569. RE_ScaI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52691-1 [UniParc]FASTAAdd to Basket

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MINDQLPRWV REARVGTRTG GPAMRPKTSD SPYFGWDSED WPEVTRQLLS    50
EQPLSGDTLV DAVLASWESI FESRLGSGFH IGTQIRPTPQ VMGFLLHALI 100
PLELANGDPS WRADLNSSEK DLVYQPDHKY SIEMKTSSHK DQIFGNRSFG 150
VENPGKGKKA KDGYYVAVNF EKWSDAPGRL PRIRTIRYGW LDHTDWVAQK 200
SQTGQQSSLP AVVSNTQLLA IHTGGQR 227
Length:227
Mass (Da):25,464
Last modified:June 1, 1998 - v1
Checksum:iA4E48072756B6009
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF044681 Genomic DNA. Translation: AAC97177.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF044681 Genomic DNA. Translation: AAC97177.1 .

3D structure databases

ModBasei Search...

Chemistry

BindingDBi O52691.
ChEMBLi CHEMBL5782.

Protein family/group databases

REBASEi 1628. ScaI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR019069. Restrct_endonuc_II_ScaI.
[Graphical view ]
Pfami PF09569. RE_ScaI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
    Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
    Mol. Gen. Genet. 260:226-231(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiT2S1_STRCS
AccessioniPrimary (citable) accession number: O52691
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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