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Protein

Type-2 restriction enzyme ScaI

Gene

scaIR

Organism
Streptomyces caespitosus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence AGTACT and cleaves after T-3.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1628. ScaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme ScaI (EC:3.1.21.4)
Short name:
R.ScaI
Alternative name(s):
Endonuclease ScaI
Type II restriction enzyme ScaI
Gene namesi
Name:scaIR
OrganismiStreptomyces caespitosus
Taxonomic identifieri53502 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Type-2 restriction enzyme ScaIPRO_0000077367Add
BLAST

Interactioni

Family & Domainsi

Family and domain databases

InterProiIPR019069. Restrct_endonuc_II_ScaI.
[Graphical view]
PfamiPF09569. RE_ScaI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52691-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINDQLPRWV REARVGTRTG GPAMRPKTSD SPYFGWDSED WPEVTRQLLS
60 70 80 90 100
EQPLSGDTLV DAVLASWESI FESRLGSGFH IGTQIRPTPQ VMGFLLHALI
110 120 130 140 150
PLELANGDPS WRADLNSSEK DLVYQPDHKY SIEMKTSSHK DQIFGNRSFG
160 170 180 190 200
VENPGKGKKA KDGYYVAVNF EKWSDAPGRL PRIRTIRYGW LDHTDWVAQK
210 220
SQTGQQSSLP AVVSNTQLLA IHTGGQR
Length:227
Mass (Da):25,464
Last modified:June 1, 1998 - v1
Checksum:iA4E48072756B6009
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044681 Genomic DNA. Translation: AAC97177.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044681 Genomic DNA. Translation: AAC97177.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiO52691.
ChEMBLiCHEMBL5782.

Protein family/group databases

REBASEi1628. ScaI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR019069. Restrct_endonuc_II_ScaI.
[Graphical view]
PfamiPF09569. RE_ScaI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli."
    Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II
    Mol. Gen. Genet. 260:226-231(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiT2S1_STRCS
AccessioniPrimary (citable) accession number: O52691
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.