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O52632 (PGK_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase

EC=2.7.2.3
Gene names
Name:pgk
Ordered Locus Names:CA_C0710
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00145

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 397397Phosphoglycerate kinase HAMAP-Rule MF_00145
PRO_0000145931

Regions

Nucleotide binding353 – 3564ATP By similarity
Region23 – 253Substrate binding By similarity
Region61 – 644Substrate binding By similarity

Sites

Binding site381Substrate By similarity
Binding site1211Substrate By similarity
Binding site1541Substrate By similarity
Binding site2051ATP By similarity
Binding site2961ATP; via carbonyl oxygen By similarity
Binding site3271ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
O52632 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 8F1C993B0447966B

FASTA39742,384
        10         20         30         40         50         60 
MKFNKKTIED VDVKGKRVLV RCDFNVPLKD GVITDENRLN GALPTIKYLS EHGGKVILCS 

        70         80         90        100        110        120 
HLGKAKGPDP SKTLAPVAKR LSELLGREVK FAADDTVVGE NAKKAVAELK EGEIVLLENT 

       130        140        150        160        170        180 
RFRPEEGKND DAFSKDLASL ADVYVNDAFG TAHRAHCSTV GVTKFVDTAV CGYLIQKELK 

       190        200        210        220        230        240 
FLGSAVENPV RPFVAILGGA KVSDKINVIN NLLEKVDTLI IGGGMAYTFL KAQGYTIGTS 

       250        260        270        280        290        300 
LLEADKVDYA KEMIEKAEAK GVKLLLPIDN IVGAEFKADT KAVTTEDANI PEGYMGLDIG 

       310        320        330        340        350        360 
PKTQKLYADA VKEAKTVVWN GPMGVFEFEN FAKGTKDVAK AMAESDATTV IGGGDSAAAV 

       370        380        390 
NQLGFGDKMT HISTGGGASL EFLEGKELPG IVALNDK 

« Hide

References

« Hide 'large scale' references
[1]"The glyceraldehyde-3-phosphate dehydrogenase of Clostridium acetobutylicum: isolation and purification of the enzyme, and sequencing and localization of the gap gene within a cluster of other glycolytic genes."
Schreiber W., Durre P.
Microbiology 145:1839-1847(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF043386 Genomic DNA. Translation: AAC13161.1.
AE001437 Genomic DNA. Translation: AAK78687.1.
PIRD96987.
RefSeqNP_347347.1. NC_003030.1.

3D structure databases

ProteinModelPortalO52632.
SMRO52632. Positions 5-397.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_C0710.

PTM databases

PhosSiteP12081777.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK78687; AAK78687; CA_C0710.
GeneID1116893.
KEGGcac:CA_C0710.
PATRIC32035787. VBICloAce74127_0913.

Phylogenomic databases

eggNOGCOG0126.
HOGENOMHOG000227108.
KOK00927.
OMAWEALDIG.
OrthoDBEOG64N9Z0.

Enzyme and pathway databases

BioCycCACE272562:GJIH-753-MONOMER.
UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGK_CLOAB
AccessionPrimary (citable) accession number: O52632
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: June 11, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways