O52632 (PGK_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	CA_C0710

Organism

Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]

Taxonomic identifier

272562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium acetobutylicum

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00145
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00145.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Phosphoglycerate kinase HAMAP-Rule MF_00145		PRO_0000145931
Regions
	Nucleotide binding	353 – 356	4	ATP By similarity
	Region	23 – 25	3	Substrate binding By similarity
	Region	61 – 64	4	Substrate binding By similarity
Sites
	Binding site	38	1	Substrate By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	205	1	ATP By similarity
	Binding site	296	1	ATP; via carbonyl oxygen By similarity
	Binding site	327	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O52632 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 8F1C993B0447966B	FASTA	397	42,384
	10 20 30 40 50 60 MKFNKKTIED VDVKGKRVLV RCDFNVPLKD GVITDENRLN GALPTIKYLS EHGGKVILCS 70 80 90 100 110 120 HLGKAKGPDP SKTLAPVAKR LSELLGREVK FAADDTVVGE NAKKAVAELK EGEIVLLENT 130 140 150 160 170 180 RFRPEEGKND DAFSKDLASL ADVYVNDAFG TAHRAHCSTV GVTKFVDTAV CGYLIQKELK 190 200 210 220 230 240 FLGSAVENPV RPFVAILGGA KVSDKINVIN NLLEKVDTLI IGGGMAYTFL KAQGYTIGTS 250 260 270 280 290 300 LLEADKVDYA KEMIEKAEAK GVKLLLPIDN IVGAEFKADT KAVTTEDANI PEGYMGLDIG 310 320 330 340 350 360 PKTQKLYADA VKEAKTVVWN GPMGVFEFEN FAKGTKDVAK AMAESDATTV IGGGDSAAAV 370 380 390 NQLGFGDKMT HISTGGGASL EFLEGKELPG IVALNDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The glyceraldehyde-3-phosphate dehydrogenase of Clostridium acetobutylicum: isolation and purification of the enzyme, and sequencing and localization of the gap gene within a cluster of other glycolytic genes." Schreiber W., Durre P. Microbiology 145:1839-1847(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF043386 Genomic DNA. Translation: AAC13161.1. AE001437 Genomic DNA. Translation: AAK78687.1.
PIR	D96987.
RefSeq	NP_347347.1. NC_003030.1.
3D structure databases
ProteinModelPortal	O52632.
SMR	O52632. Positions 5-397.
ModBase	Search...
Protein-protein interaction databases
STRING	272562.CA_C0710.
PTM databases
PhosSite	P12081777.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK78687; AAK78687; CA_C0710.
GeneID	1116893.
KEGG	cac:CA_C0710.
PATRIC	32035787. VBICloAce74127_0913.
Phylogenomic databases
eggNOG	COG0126.
HOGENOM	HOG000227108.
KO	K00927.
OMA	KFGLADK.
ProtClustDB	PRK00073.
Enzyme and pathway databases
BioCyc	CACE272562:GJIH-753-MONOMER.
UniPathway	UPA00109; UER00185.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
HAMAP	MF_00145. Phosphoglyc_kinase.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_CLOAB

Accession

Primary (citable) accession number: O52632

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program