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O52629

- BGAL_PYRWO

UniProt

O52629 - BGAL_PYRWO

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Protein

Beta-galactosidase

Gene
N/A
Organism
Pyrococcus woesei
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Temperature dependencei

This enzyme is thermostable.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei210 – 2101Proton donorSequence Analysis
Active sitei414 – 4141NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.23. 5249.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Lactase
OrganismiPyrococcus woesei
Taxonomic identifieri2262 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 510510Beta-galactosidasePRO_0000063865Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO52629.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 2 hits.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52629-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFPEKFLWGV AQSGFQFEMG DKLRRNIDTN TDWWHWVRDK TNIEKGLVSG
60 70 80 90 100
DLPEEGINNY ELYEKDHEIA RKLGLNAYRI GIEWSRIFPW PTTFIDVDYS
110 120 130 140 150
YNESYNLIED VKITKDTLEE LDEIANKREV AYYRSVINSL RSKGFKVIVN
160 170 180 190 200
LNHFTLPYWL HDPIEARERA LTNKRNGWVN PRTVIEFAKY AAYIAYKFGD
210 220 230 240 250
IVDMWSTFNE PMVVVELGYL APYSGFPPGV LNPEAAKLAI LHMINAHALA
260 270 280 290 300
YRQIKKFDTE KADKDSKEPA EVGIIYNNIG VAYPKDPNDS KDVKAAENDN
310 320 330 340 350
FFHSGLFFEA IHKGKLNIEF DGETFIDAPY LKGNDWIGVN YYTREVVTYQ
360 370 380 390 400
EPMFPSIPLI TFKGVQGYGY ACRPGTLSKD DRPVSDIGWE LYPEGMYDSI
410 420 430 440 450
VEAHKYGVPV YVTENGIADS KDILRPYYIA SHIKMTEKAF EDGYEVKGYF
460 470 480 490 500
HWALTDNFEW ALGFRMRFGL YEVNLITKER IPREKSVSIF REIVANNGVT
510
KKIEEELLRG
Length:510
Mass (Da):59,057
Last modified:June 1, 1998 - v1
Checksum:i10D0D3B8D9F26545
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF043283 Genomic DNA. Translation: AAB97862.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF043283 Genomic DNA. Translation: AAB97862.1 .

3D structure databases

ProteinModelPortali O52629.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 3.2.1.23. 5249.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 2 hits.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, expression, and purification of the His(6)-tagged thermostable beta-galactosidase from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme."
    Daabrowski S., Sobiewska G., Maciunska J., Synowiecki J., Kur J.
    Protein Expr. Purif. 19:107-112(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: DSM 3773.

Entry informationi

Entry nameiBGAL_PYRWO
AccessioniPrimary (citable) accession number: O52629
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 1998
Last modified: October 1, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3