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O52629

- BGAL_PYRWO

UniProt

O52629 - BGAL_PYRWO

Protein

Beta-galactosidase

Gene
N/A
Organism
Pyrococcus woesei
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Temperature dependencei

    This enzyme is thermostable.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei210 – 2101Proton donorSequence Analysis
    Active sitei414 – 4141NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BRENDAi3.2.1.23. 5249.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase (EC:3.2.1.23)
    Short name:
    Lactase
    OrganismiPyrococcus woesei
    Taxonomic identifieri2262 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 510510Beta-galactosidasePRO_0000063865Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliO52629.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 2 hits.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O52629-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFPEKFLWGV AQSGFQFEMG DKLRRNIDTN TDWWHWVRDK TNIEKGLVSG    50
    DLPEEGINNY ELYEKDHEIA RKLGLNAYRI GIEWSRIFPW PTTFIDVDYS 100
    YNESYNLIED VKITKDTLEE LDEIANKREV AYYRSVINSL RSKGFKVIVN 150
    LNHFTLPYWL HDPIEARERA LTNKRNGWVN PRTVIEFAKY AAYIAYKFGD 200
    IVDMWSTFNE PMVVVELGYL APYSGFPPGV LNPEAAKLAI LHMINAHALA 250
    YRQIKKFDTE KADKDSKEPA EVGIIYNNIG VAYPKDPNDS KDVKAAENDN 300
    FFHSGLFFEA IHKGKLNIEF DGETFIDAPY LKGNDWIGVN YYTREVVTYQ 350
    EPMFPSIPLI TFKGVQGYGY ACRPGTLSKD DRPVSDIGWE LYPEGMYDSI 400
    VEAHKYGVPV YVTENGIADS KDILRPYYIA SHIKMTEKAF EDGYEVKGYF 450
    HWALTDNFEW ALGFRMRFGL YEVNLITKER IPREKSVSIF REIVANNGVT 500
    KKIEEELLRG 510
    Length:510
    Mass (Da):59,057
    Last modified:June 1, 1998 - v1
    Checksum:i10D0D3B8D9F26545
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF043283 Genomic DNA. Translation: AAB97862.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF043283 Genomic DNA. Translation: AAB97862.1 .

    3D structure databases

    ProteinModelPortali O52629.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 3.2.1.23. 5249.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 2 hits.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression, and purification of the His(6)-tagged thermostable beta-galactosidase from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme."
      Daabrowski S., Sobiewska G., Maciunska J., Synowiecki J., Kur J.
      Protein Expr. Purif. 19:107-112(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
      Strain: DSM 3773.

    Entry informationi

    Entry nameiBGAL_PYRWO
    AccessioniPrimary (citable) accession number: O52629
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3