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O52629 (BGAL_PYRWO) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Lactase
EC=3.2.1.23
OrganismPyrococcus woesei
Taxonomic identifier2262 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Biophysicochemical properties

Temperature dependence:

This enzyme is thermostable.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 510510Beta-galactosidase
PRO_0000063865

Sites

Active site2101Proton donor Potential
Active site4141Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
O52629 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 10D0D3B8D9F26545

FASTA51059,057
        10         20         30         40         50         60 
MFPEKFLWGV AQSGFQFEMG DKLRRNIDTN TDWWHWVRDK TNIEKGLVSG DLPEEGINNY 

        70         80         90        100        110        120 
ELYEKDHEIA RKLGLNAYRI GIEWSRIFPW PTTFIDVDYS YNESYNLIED VKITKDTLEE 

       130        140        150        160        170        180 
LDEIANKREV AYYRSVINSL RSKGFKVIVN LNHFTLPYWL HDPIEARERA LTNKRNGWVN 

       190        200        210        220        230        240 
PRTVIEFAKY AAYIAYKFGD IVDMWSTFNE PMVVVELGYL APYSGFPPGV LNPEAAKLAI 

       250        260        270        280        290        300 
LHMINAHALA YRQIKKFDTE KADKDSKEPA EVGIIYNNIG VAYPKDPNDS KDVKAAENDN 

       310        320        330        340        350        360 
FFHSGLFFEA IHKGKLNIEF DGETFIDAPY LKGNDWIGVN YYTREVVTYQ EPMFPSIPLI 

       370        380        390        400        410        420 
TFKGVQGYGY ACRPGTLSKD DRPVSDIGWE LYPEGMYDSI VEAHKYGVPV YVTENGIADS 

       430        440        450        460        470        480 
KDILRPYYIA SHIKMTEKAF EDGYEVKGYF HWALTDNFEW ALGFRMRFGL YEVNLITKER 

       490        500        510 
IPREKSVSIF REIVANNGVT KKIEEELLRG 

« Hide

References

[1]"Cloning, expression, and purification of the His(6)-tagged thermostable beta-galactosidase from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme."
Daabrowski S., Sobiewska G., Maciunska J., Synowiecki J., Kur J.
Protein Expr. Purif. 19:107-112(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
Strain: DSM 3773.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF043283 Genomic DNA. Translation: AAB97862.1.

3D structure databases

ProteinModelPortalO52629.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA3.2.1.23. 5249.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 2 hits.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_PYRWO
AccessionPrimary (citable) accession number: O52629
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 1998
Last modified: October 16, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries