O52629 (BGAL_PYRWO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Beta-galactosidase Short name=Lactase EC=3.2.1.23
Organism	Pyrococcus woesei
Taxonomic identifier	2262 [NCBI]
Taxonomic lineage	Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.
Biophysicochemical properties	Temperature dependence: This enzyme is thermostable.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Beta-galactosidase		PRO_0000063865
Sites
	Active site	210	1	Proton donor Potential
	Active site	414	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	O52629 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 10D0D3B8D9F26545	FASTA	510	59,057
	10 20 30 40 50 60 MFPEKFLWGV AQSGFQFEMG DKLRRNIDTN TDWWHWVRDK TNIEKGLVSG DLPEEGINNY 70 80 90 100 110 120 ELYEKDHEIA RKLGLNAYRI GIEWSRIFPW PTTFIDVDYS YNESYNLIED VKITKDTLEE 130 140 150 160 170 180 LDEIANKREV AYYRSVINSL RSKGFKVIVN LNHFTLPYWL HDPIEARERA LTNKRNGWVN 190 200 210 220 230 240 PRTVIEFAKY AAYIAYKFGD IVDMWSTFNE PMVVVELGYL APYSGFPPGV LNPEAAKLAI 250 260 270 280 290 300 LHMINAHALA YRQIKKFDTE KADKDSKEPA EVGIIYNNIG VAYPKDPNDS KDVKAAENDN 310 320 330 340 350 360 FFHSGLFFEA IHKGKLNIEF DGETFIDAPY LKGNDWIGVN YYTREVVTYQ EPMFPSIPLI 370 380 390 400 410 420 TFKGVQGYGY ACRPGTLSKD DRPVSDIGWE LYPEGMYDSI VEAHKYGVPV YVTENGIADS 430 440 450 460 470 480 KDILRPYYIA SHIKMTEKAF EDGYEVKGYF HWALTDNFEW ALGFRMRFGL YEVNLITKER 490 500 510 IPREKSVSIF REIVANNGVT KKIEEELLRG

References

[1]	"Cloning, expression, and purification of the His(6)-tagged thermostable beta-galactosidase from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme." Daabrowski S., Sobiewska G., Maciunska J., Synowiecki J., Kur J. Protein Expr. Purif. 19:107-112(2000) [PubMed: 10833397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: DSM 3773.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF043283 Genomic DNA. Translation: AAB97862.1.
3D structure databases
ProteinModelPortal	O52629.
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	3.2.1.23. 5249.
Family and domain databases
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 2 hits. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_PYRWO

Accession

Primary (citable) accession number: O52629

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	June 1, 1998
Last modified:	September 21, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program