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Protein

Guanine nucleotide exchange factor SopE

Gene

sopE

Organism
Salmonella typhimurium
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activator for both CDC42 and RAC1 by directly engaging these Rho GTPases and acting as potent guanine nucleotide exchange factor (GEF). This activation results in actin cytoskeleton rearrangements and stimulates membrane ruffling, promoting bacterial entry into non-phagocytic cells. Also activates MAPK8, indicating that it is capable of stimulating signaling pathways that can lead to nuclear responses. Chaperone InvB is required for secretion and translocation of SopE.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei103 – 1031Helps stabilization of SopE-CDC42
Sitei109 – 1091Helps stabilization of SopE-CDC42
Sitei124 – 1241Helps stabilization of SopE-CDC42
Sitei131 – 1311Helps stabilization of SopE-CDC42
Sitei174 – 1741Helps stabilization of SopE-CDC42
Sitei194 – 1941Helps stabilization of SopE-CDC42
Sitei198 – 1981Helps stabilization of SopE-CDC42

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Guanine-nucleotide releasing factor

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide exchange factor SopE
Alternative name(s):
Effector protein SopE
Toxin SopE
Gene namesi
Name:sopE
OrganismiSalmonella typhimurium
Taxonomic identifieri90371 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi103 – 1031D → A: 4-fold reduction in GEF activity. 1 Publication
Mutagenesisi103 – 1031D → E: 2-fold reduction in GEF activity. 1 Publication
Mutagenesisi109 – 1091Q → A: 700-fold reduction in GEF activity. 1 Publication
Mutagenesisi109 – 1091Q → N: 160-fold reduction in GEF activity. 1 Publication
Mutagenesisi124 – 1241D → A or E: 160-800-fold reduction in GEF activity. 1 Publication
Mutagenesisi168 – 1681G → A: 5000-fold reduction in GEF activity. 1 Publication
Mutagenesisi168 – 1681G → V: 20000-fold reduction in GEF activity. 1 Publication
Mutagenesisi168 – 1681Missing : 800-fold reduction in GEF activity. 1 Publication
Mutagenesisi198 – 1981K → A or R: No effect. 1 Publication
Mutagenesisi198 – 1981K → E: More than 20-fold reduction in GEF activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 240239Guanine nucleotide exchange factor SopEPRO_0000220740Add
BLAST

Expressioni

Inductioni

Transcriptionally activated by InvF in association with SicA.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC42P609532EBI-602254,EBI-81752From a different organism.

Protein-protein interaction databases

IntActiO52623. 1 interaction.

Structurei

Secondary structure

1
240
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi80 – 9415Combined sources
Helixi96 – 1027Combined sources
Helixi104 – 13229Combined sources
Helixi138 – 15013Combined sources
Beta strandi155 – 1584Combined sources
Beta strandi161 – 1644Combined sources
Helixi173 – 18311Combined sources
Helixi185 – 1884Combined sources
Helixi191 – 20919Combined sources
Helixi210 – 2134Combined sources
Helixi221 – 23616Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GZSX-ray2.30B/D78-240[»]
ProteinModelPortaliO52623.
SMRiO52623. Positions 78-240.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO52623.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 7864Interaction with InvBAdd
BLAST
Regioni78 – 240163GEF catalytic domainAdd
BLAST
Regioni165 – 1728Catalytic loop involved in contact with CDC42
Regioni166 – 1694Core-loop involved in conformational changes in complex with CDC42

Sequence similaritiesi

Family and domain databases

InterProiIPR005414. SopE.
IPR016019. SopE_GEF_dom.
IPR016018. SopE_N_dom.
[Graphical view]
PfamiPF05364. SecIII_SopE_N. 1 hit.
PF07487. SopE_GEF. 1 hit.
[Graphical view]
PIRSFiPIRSF034781. SecIII_sopE. 1 hit.
PRINTSiPR01593. SOPEPROTEIN.
SUPFAMiSSF81832. SSF81832. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O52623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKITLSPQN FRIQKQETTL LKEKSTEKNS LAKSILAVKN HFIELRSKLS
60 70 80 90 100
ERFISHKNTE SSATHFHRGS ASEGRAVLTN KVVKDFMLQT LNDIDIRGSA
110 120 130 140 150
SKDPAYASQT REAILSAVYS KNKDQCCNLL ISKGINIAPF LQEIGEAAKN
160 170 180 190 200
AGLPGTTKND VFTPSGAGAN PFITPLISSA NSKYPRMFIN QHQQASFKIY
210 220 230 240
AEKIIMTEVA PLFNECAMPT PQQFQLILEN IANKYIQNTP
Length:240
Mass (Da):26,636
Last modified:January 23, 2007 - v3
Checksum:iF636F6C437018D03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043239 Genomic DNA. Translation: AAC02071.1.
RefSeqiWP_000161707.1. NZ_LVGC01000014.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043239 Genomic DNA. Translation: AAC02071.1.
RefSeqiWP_000161707.1. NZ_LVGC01000014.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GZSX-ray2.30B/D78-240[»]
ProteinModelPortaliO52623.
SMRiO52623. Positions 78-240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO52623. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO52623.

Family and domain databases

InterProiIPR005414. SopE.
IPR016019. SopE_GEF_dom.
IPR016018. SopE_N_dom.
[Graphical view]
PfamiPF05364. SecIII_SopE_N. 1 hit.
PF07487. SopE_GEF. 1 hit.
[Graphical view]
PIRSFiPIRSF034781. SecIII_sopE. 1 hit.
PRINTSiPR01593. SOPEPROTEIN.
SUPFAMiSSF81832. SSF81832. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSOPE_SALTM
AccessioniPrimary (citable) accession number: O52623
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 80 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Encoded within a prophage region, which is only present in very few Salmonella isolates. SopE-expressing S.typhimurium strains are associated with severe epidemics.

Caution

SopE is not present in the complete genome strain LT2.Curated

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.