Reviewed, UniProtKB/Swiss-Prot O52597 (DUT_BRAJA)

Names and origin

Protein names

Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolase
      Short name=dUTPase
    EC=3.6.1.23
Alternative name(s):
    dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	bll0758

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity. HAMAP MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116		PRO_0000182834
Regions
	Region	72 – 74	3	Substrate binding By similarity
	Region	89 – 91	3	Substrate binding By similarity
Sites
	Binding site	85	1	Substrate By similarity
	Binding site	99	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
Experimental info
	Sequence conflict	60	1	A → V in AAB97415. Ref.1
	Sequence conflict	109 – 120	12	AFVIK…ERIAQ → VLRDQARRADRAK Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O52597-1 [UniParc]. Last modified February 28, 2003. Version 2. Checksum: 0270434059D7B3CE	FASTA	152	15,574
	10 20 30 40 50 60 MSTKVTVELQ RLPHAEGLPL PAYQTAEAAG LDLMAAVPED APLTLASGRY ALVPTGLAIA 70 80 90 100 110 120 LPPGHEAQVR PRSGLAAKHG VTVLNSPGTI DADYRGEIKV ILINHGAAAF VIKRGERIAQ 130 140 150 MVIAPVVQAA LVPVATLSAT DRGAGGFGST GR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"BdfA, a novel sensor protein of Bradyrhizobium japonicum, is required for normal bacteroid differentiation in soybean (Glycine max)." Mueller P., Unnewehr A. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110spc4.
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF042096 Genomic DNA. Translation: AAB97415.1. BA000040 Genomic DNA. Translation: BAC46023.1.
RefSeq	NP_767398.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1049768.
GenomeReviews	Gene locus bll0758 in contig BA000040_GR.
KEGG	bja:bll0758.
NMPDR	fig|224911.1.peg.758.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG436079.
OMA	GTIDEGY.
PhylomeDB	O52597.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL0758-MONOMER.
BRENDA	3.6.1.23. 280.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. DeoxyUTP_pyroPase_dom. IPR008181. dUTP_pyrophosphatase_subfam_1. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_BRAJA

Accession

Primary (citable) accession number: O52597

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 28, 2003
Last modified:	February 9, 2010
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)