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O52597

- DUT_BRADU

UniProt

O52597 - DUT_BRADU

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Protein
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Gene
dut, bll0758
Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity.UniRule annotation

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851Substrate By similarity
Binding sitei99 – 991Substrate; via amide nitrogen and carbonyl oxygen By similarity

GO - Molecular functioni

  1. dUTP diphosphatase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. dUMP biosynthetic process Source: UniProtKB-UniPathway
  2. dUTP metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-766-MONOMER.
UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
Gene namesi
Name:dut
Ordered Locus Names:bll0758
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation
PRO_0000182834Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224911.bll0758.

Structurei

3D structure databases

ProteinModelPortaliO52597.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni72 – 743Substrate binding By similarity
Regioni89 – 913Substrate binding By similarity

Sequence similaritiesi

Belongs to the dUTPase family.

Phylogenomic databases

eggNOGiCOG0756.
HOGENOMiHOG000028966.
KOiK01520.
OrthoDBiEOG689HXK.
PhylomeDBiO52597.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
HAMAPiMF_00116. dUTPase_bact.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

O52597-1 [UniParc]FASTAAdd to Basket

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MSTKVTVELQ RLPHAEGLPL PAYQTAEAAG LDLMAAVPED APLTLASGRY    50
ALVPTGLAIA LPPGHEAQVR PRSGLAAKHG VTVLNSPGTI DADYRGEIKV 100
ILINHGAAAF VIKRGERIAQ MVIAPVVQAA LVPVATLSAT DRGAGGFGST 150
GR 152
Length:152
Mass (Da):15,574
Last modified:February 28, 2003 - v2
Checksum:i0270434059D7B3CE
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601A → V in AAB97415. 1 Publication
Sequence conflicti109 – 12012AFVIK…ERIAQ → VLRDQARRADRAK1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF042096 Genomic DNA. Translation: AAB97415.1.
BA000040 Genomic DNA. Translation: BAC46023.1.
RefSeqiNP_767398.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC46023; BAC46023; BAC46023.
GeneIDi1049768.
KEGGibja:bll0758.
PATRICi21185020. VBIBraJap65052_0782.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF042096 Genomic DNA. Translation: AAB97415.1 .
BA000040 Genomic DNA. Translation: BAC46023.1 .
RefSeqi NP_767398.1. NC_004463.1.

3D structure databases

ProteinModelPortali O52597.
ModBasei Search...

Protein-protein interaction databases

STRINGi 224911.bll0758.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC46023 ; BAC46023 ; BAC46023 .
GeneIDi 1049768.
KEGGi bja:bll0758.
PATRICi 21185020. VBIBraJap65052_0782.

Phylogenomic databases

eggNOGi COG0756.
HOGENOMi HOG000028966.
KOi K01520.
OrthoDBi EOG689HXK.
PhylomeDBi O52597.

Enzyme and pathway databases

UniPathwayi UPA00610 ; UER00666 .
BioCyci BJAP224911:GJEJ-766-MONOMER.

Family and domain databases

Gene3Di 2.70.40.10. 1 hit.
HAMAPi MF_00116. dUTPase_bact.
InterProi IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view ]
Pfami PF00692. dUTPase. 1 hit.
[Graphical view ]
SUPFAMi SSF51283. SSF51283. 1 hit.
TIGRFAMsi TIGR00576. dut. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "BdfA, a novel sensor protein of Bradyrhizobium japonicum, is required for normal bacteroid differentiation in soybean (Glycine max)."
    Mueller P., Unnewehr A.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: USDA 110spc4.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiDUT_BRADU
AccessioniPrimary (citable) accession number: O52597
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 28, 2003
Last modified: July 9, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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