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Protein

Acetate kinase

Gene

ackA

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg2+. Can also accept Mn2+.UniRule annotation

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. Phosphate acetyltransferase (pta)
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7MagnesiumUniRule annotation1
Binding sitei14ATPUniRule annotation1
Binding sitei91SubstrateUniRule annotation1
Active sitei148Proton donor/acceptorUniRule annotation1
Sitei180Transition state stabilizerUniRule annotation1
Sitei241Transition state stabilizerUniRule annotation1
Metal bindingi384MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi208 – 212ATPUniRule annotation5
Nucleotide bindingi283 – 285ATPUniRule annotation3
Nucleotide bindingi331 – 335ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTHE203119:G1G86-1071-MONOMER
UniPathwayiUPA00340; UER00458

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Synonyms:ack
Ordered Locus Names:Cthe_1028
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001075491 – 399Acetate kinaseAdd BLAST399

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi203119.Cthe_1028

Structurei

3D structure databases

ProteinModelPortaliO52594
SMRiO52594
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6H Bacteria
COG0282 LUCA
HOGENOMiHOG000288399
KOiK00925
OMAiWKALTGT
OrthoDBiPOG091H02K4

Family and domain databases

HAMAPiMF_00020 Acetate_kinase, 1 hit
InterProiView protein in InterPro
IPR004372 Ac/propionate_kinase
IPR000890 Aliphatic_acid_kin_short-chain
IPR023865 Aliphatic_acid_kinase_CS
PANTHERiPTHR21060 PTHR21060, 1 hit
PfamiView protein in Pfam
PF00871 Acetate_kinase, 1 hit
PIRSFiPIRSF000722 Acetate_prop_kin, 1 hit
PRINTSiPR00471 ACETATEKNASE
TIGRFAMsiTIGR00016 ackA, 1 hit
PROSITEiView protein in PROSITE
PS01075 ACETATE_KINASE_1, 1 hit
PS01076 ACETATE_KINASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

O52594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNILVINTGS SSLKYQLIDM TNESVLAKGV CDRIGLEHSF LKHTKTGGET
60 70 80 90 100
VVIEKDLYNH KLAIQEVISA LTDEKIGVIK SMSEISAVGH RIVHGGEKFK
110 120 130 140 150
ESAIIDEDVM KAIRDCVELA PLHNPSNIIG IEACKQILPD VPMVAVFDTA
160 170 180 190 200
FHQTMPRHAY IYALPYEIYE KYKLRKYGFH GTSHKYVAHR AAQMLGKPIE
210 220 230 240 250
SLKLITCHLG NGASICAVKG GKSVDTSMGF TPLQGLCMGT RSGNVDPAVI
260 270 280 290 300
TYLMEKEKMN INDINNFLNK KSGVLGISGV SSDFRDVQDA AEKGDDRAQL
310 320 330 340 350
ALDIFCYGVR KYIGKYIAVL NGVDAVVFTA GIGENNAYIR REVLKDMDFF
360 370 380 390
GIKIDLDKNE VKGKEADISA PDAKVKTLVI PTNEELEIAR ETLRLVKNL
Length:399
Mass (Da):43,966
Last modified:June 1, 1998 - v1
Checksum:i1DEF07FA0A72226B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041841 Genomic DNA Translation: AAB96952.1
CP000568 Genomic DNA Translation: ABN52260.1
RefSeqiWP_003515609.1, NC_009012.1

Genome annotation databases

EnsemblBacteriaiABN52260; ABN52260; Cthe_1028
GeneIDi35803776
KEGGicth:Cthe_1028

Similar proteinsi

Entry informationi

Entry nameiACKA_CLOTH
AccessioniPrimary (citable) accession number: O52594
Secondary accession number(s): A3DE81
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: April 25, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health