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Protein

Acetate kinase

Gene

ackA

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg2+. Can also accept Mn2+.UniRule annotation

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. Phosphate acetyltransferase (pta)
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71MagnesiumUniRule annotation
Binding sitei14 – 141ATPUniRule annotation
Binding sitei91 – 911SubstrateUniRule annotation
Active sitei148 – 1481Proton donor/acceptorUniRule annotation
Sitei180 – 1801Transition state stabilizerUniRule annotation
Sitei241 – 2411Transition state stabilizerUniRule annotation
Metal bindingi384 – 3841MagnesiumUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi208 – 2125ATPUniRule annotation
Nucleotide bindingi283 – 2853ATPUniRule annotation
Nucleotide bindingi331 – 3355ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-1064-MONOMER.
UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Synonyms:ack
Ordered Locus Names:Cthe_1028
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 399399Acetate kinasePRO_0000107549Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi203119.Cthe_1028.

Structurei

3D structure databases

ProteinModelPortaliO52594.
SMRiO52594. Positions 1-397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6H. Bacteria.
COG0282. LUCA.
HOGENOMiHOG000288399.
KOiK00925.
OMAiHRLKKYI.
OrthoDBiPOG091H02K4.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiIPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNILVINTGS SSLKYQLIDM TNESVLAKGV CDRIGLEHSF LKHTKTGGET
60 70 80 90 100
VVIEKDLYNH KLAIQEVISA LTDEKIGVIK SMSEISAVGH RIVHGGEKFK
110 120 130 140 150
ESAIIDEDVM KAIRDCVELA PLHNPSNIIG IEACKQILPD VPMVAVFDTA
160 170 180 190 200
FHQTMPRHAY IYALPYEIYE KYKLRKYGFH GTSHKYVAHR AAQMLGKPIE
210 220 230 240 250
SLKLITCHLG NGASICAVKG GKSVDTSMGF TPLQGLCMGT RSGNVDPAVI
260 270 280 290 300
TYLMEKEKMN INDINNFLNK KSGVLGISGV SSDFRDVQDA AEKGDDRAQL
310 320 330 340 350
ALDIFCYGVR KYIGKYIAVL NGVDAVVFTA GIGENNAYIR REVLKDMDFF
360 370 380 390
GIKIDLDKNE VKGKEADISA PDAKVKTLVI PTNEELEIAR ETLRLVKNL
Length:399
Mass (Da):43,966
Last modified:June 1, 1998 - v1
Checksum:i1DEF07FA0A72226B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041841 Genomic DNA. Translation: AAB96952.1.
CP000568 Genomic DNA. Translation: ABN52260.1.
RefSeqiWP_003515609.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN52260; ABN52260; Cthe_1028.
KEGGicth:Cthe_1028.
PATRICi19515789. VBICloThe47081_1080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041841 Genomic DNA. Translation: AAB96952.1.
CP000568 Genomic DNA. Translation: ABN52260.1.
RefSeqiWP_003515609.1. NC_009012.1.

3D structure databases

ProteinModelPortaliO52594.
SMRiO52594. Positions 1-397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203119.Cthe_1028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN52260; ABN52260; Cthe_1028.
KEGGicth:Cthe_1028.
PATRICi19515789. VBICloThe47081_1080.

Phylogenomic databases

eggNOGiENOG4105C6H. Bacteria.
COG0282. LUCA.
HOGENOMiHOG000288399.
KOiK00925.
OMAiHRLKKYI.
OrthoDBiPOG091H02K4.

Enzyme and pathway databases

UniPathwayiUPA00340; UER00458.
BioCyciCTHE203119:GIW8-1064-MONOMER.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiIPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACKA_CLOTH
AccessioniPrimary (citable) accession number: O52594
Secondary accession number(s): A3DE81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: September 7, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.