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Protein

Coenzyme A disulfide reductase

Gene

cdr

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes specifically the NADPH-dependent reduction of coenzyme A disulfide. Is also active with other disulfide substrates containing at least one 4'-phosphopantethienyl moiety such as 4,4'-diphosphopantethine, but is not able to reduce oxidized glutathione, cystine, pantethine, or H2O2.

Miscellaneous

Reduction of disulfides occurs by a thiol-disulfide exchange reaction, but involves only a single catalytic cysteine residue that forms a stable mixed disulfide with CoA during catalysis.

Catalytic activityi

2 CoA + NADP+ = CoA-disulfide + NADPH.

Cofactori

FADNote: Binds 1 FAD per subunit.

Kineticsi

  1. KM=2 µM for NADPH
  2. KM=11 µM for CoA disulfide
  3. KM=140 µM for 3'-dephospho-CoA disulfide
  4. KM=80 µM for 4,4'-diphosphopantethine
  5. KM=1100 µM for CoA glutathione mixed disulfide

    pH dependencei

    Optimum pH is 7.0-8.0.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei15Substrate1 Publication1
    Binding sitei19Substrate1 Publication1
    Binding sitei22Substrate1 Publication1
    Binding sitei39Substrate1 Publication1
    Binding sitei42Substrate1 Publication1
    Active sitei43Nucleophile1 Publication1
    Active sitei43Redox-active1 Publication1
    Binding sitei71Substrate1 Publication1
    Binding sitei299Substrate1 Publication1
    Binding sitei419FAD; via carbonyl oxygen1 Publication1
    Binding sitei427Substrate1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi8 – 33FAD1 PublicationAdd BLAST26
    Nucleotide bindingi151 – 166NADPBy similarityAdd BLAST16
    Nucleotide bindingi267 – 277FAD1 PublicationAdd BLAST11

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    LigandFAD, Flavoprotein, NADP

    Enzyme and pathway databases

    BioCyciSAUR93061:G1G5Y-851-MONOMER
    BRENDAi1.8.1.14 3352
    SABIO-RKiO52582

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Coenzyme A disulfide reductase (EC:1.8.1.14)
    Short name:
    CoA-disulfide reductase
    Short name:
    CoADR
    Gene namesi
    Name:cdr
    Ordered Locus Names:SAOUHSC_00908
    OrganismiStaphylococcus aureus (strain NCTC 8325)
    Taxonomic identifieri93061 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
    Proteomesi

    Subcellular locationi

    GO - Cellular componenti

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi43C → S: Loss of activity. 1 Publication1
    Mutagenesisi361Y → F: Reduces activity by 92%. Loss of activity; when associated with F-419. 1 Publication1
    Mutagenesisi419Y → F: Reduces activity by 80%. Loss of activity; when associated with F-361. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00001846882 – 438Coenzyme A disulfide reductaseAdd BLAST437

    Proteomic databases

    PRIDEiO52582

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi93061.SAOUHSC_00908

    Structurei

    Secondary structure

    1438
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 7Combined sources4
    Helixi13 – 23Combined sources11
    Beta strandi25 – 27Combined sources3
    Beta strandi29 – 36Combined sources8
    Beta strandi38 – 40Combined sources3
    Helixi42 – 44Combined sources3
    Helixi45 – 49Combined sources5
    Helixi56 – 59Combined sources4
    Helixi64 – 71Combined sources8
    Beta strandi74 – 77Combined sources4
    Beta strandi79 – 85Combined sources7
    Turni86 – 89Combined sources4
    Beta strandi90 – 95Combined sources6
    Turni96 – 99Combined sources4
    Beta strandi100 – 105Combined sources6
    Beta strandi107 – 111Combined sources5
    Beta strandi115 – 117Combined sources3
    Helixi133 – 146Combined sources14
    Beta strandi150 – 154Combined sources5
    Helixi158 – 170Combined sources13
    Beta strandi173 – 181Combined sources9
    Helixi189 – 192Combined sources4
    Helixi193 – 201Combined sources9
    Beta strandi206 – 209Combined sources4
    Beta strandi212 – 216Combined sources5
    Beta strandi219 – 222Combined sources4
    Beta strandi227 – 229Combined sources3
    Beta strandi231 – 235Combined sources5
    Beta strandi239 – 242Combined sources4
    Helixi244 – 246Combined sources3
    Beta strandi272 – 274Combined sources3
    Helixi276 – 278Combined sources3
    Beta strandi279 – 290Combined sources12
    Helixi295 – 310Combined sources16
    Beta strandi324 – 328Combined sources5
    Beta strandi331 – 338Combined sources8
    Helixi340 – 345Combined sources6
    Beta strandi348 – 358Combined sources11
    Beta strandi362 – 364Combined sources3
    Beta strandi367 – 375Combined sources9
    Turni376 – 378Combined sources3
    Beta strandi380 – 391Combined sources12
    Helixi392 – 404Combined sources13
    Helixi410 – 414Combined sources5
    Turni421 – 423Combined sources3
    Helixi429 – 435Combined sources7

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1YQZX-ray1.54A/B1-438[»]
    ProteinModelPortaliO52582
    SMRiO52582
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO52582

    Family & Domainsi

    Domaini

    Sequence similaritiesi

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    eggNOGiENOG4107QMW Bacteria
    COG0446 LUCA
    HOGENOMiHOG000276710
    KOiK08255

    Family and domain databases

    Gene3Di3.50.50.60, 6 hits
    HAMAPiMF_01608 CoA_diS_reduct, 1 hit
    InterProiView protein in InterPro
    IPR017758 CoA_disulphide_reductase
    IPR023536 CoA_disulphide_reductase_staph
    IPR036188 FAD/NAD-bd_sf
    IPR023753 FAD/NAD-binding_dom
    IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
    IPR004099 Pyr_nucl-diS_OxRdtase_dimer
    PfamiView protein in Pfam
    PF07992 Pyr_redox_2, 1 hit
    PF02852 Pyr_redox_dim, 1 hit
    SUPFAMiSSF51905 SSF51905, 1 hit
    SSF55424 SSF55424, 1 hit
    TIGRFAMsiTIGR03385 CoA_CoA_reduc, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O52582-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPKIVVVGAV AGGATCASQI RRLDKESDII IFEKDRDMSF ANCALPYVIG
    60 70 80 90 100
    EVVEDRRYAL AYTPEKFYDR KQITVKTYHE VIAINDERQT VSVLNRKTNE
    110 120 130 140 150
    QFEESYDKLI LSPGASANSL GFESDITFTL RNLEDTDAID QFIKANQVDK
    160 170 180 190 200
    VLVVGAGYVS LEVLENLNER GLHPTLIHRS DKINKLMDAD MNQPILDELD
    210 220 230 240 250
    KREIPYRLNE EINAINGNEI TFKSGKVEHY DMIIEGVGTH PNSKFIESSN
    260 270 280 290 300
    IKLDRKGFIP VNDKFETNVP NIYAIGDIAT SHYRHVDLPA SVPLAWGAHR
    310 320 330 340 350
    AASIVAEQIA GNDTIEFKGF LGNNIVKFFD YTFASVGVKP NELKQFDYKM
    360 370 380 390 400
    VEVTQGAHAN YYPGNSPLHL RVYYDTSNRQ ILRAAAVGKE GADKRIDVLS
    410 420 430
    MAMMNQLTVD ELTEFEVAYA PPYSHPKDLI NMIGYKAK
    Length:438
    Mass (Da):49,241
    Last modified:January 23, 2007 - v3
    Checksum:i3A9B643BD8D44402
    GO

    Mass spectrometryi

    Molecular mass is 49153 Da from positions 2 - 438. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF041467 Genomic DNA Translation: AAB97073.1
    CP000253 Genomic DNA Translation: ABD30033.1
    RefSeqiYP_499461.1, NC_007795.1

    Genome annotation databases

    EnsemblBacteriaiABD30033; ABD30033; SAOUHSC_00908
    GeneIDi3920795
    KEGGisao:SAOUHSC_00908
    PATRICifig|93061.5.peg.829

    Similar proteinsi

    Entry informationi

    Entry nameiCDR_STAA8
    AccessioniPrimary (citable) accession number: O52582
    Secondary accession number(s): Q2FZT2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: January 23, 2007
    Last modified: May 23, 2018
    This is version 115 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

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