O52549 (O52549_AMYMD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
3-dehydroquinate synthase HAMAP MF_00110
EC=4.2.3.4 HAMAP MF_00110

Gene names

Name:	rifG EMBL AAC01717.1
Synonyms:	aroB HAMAP MF_00110

Organism

Amycolatopsis mediterranei (Nocardia mediterranei) EMBL AAC01717.1

Taxonomic identifier

33910 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Amycolatopsis

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate. HAMAP MF_00110
Cofactor	Divalent metal cations By similarity. HAMAP MF_00110 NAD By similarity. HAMAP MF_00110
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7. HAMAP MF_00110
Subunit structure	Monomer By similarity. HAMAP MF_00110
Subcellular location	Cytoplasm By similarity HAMAP MF_00110.
Sequence similarities	Belongs to the dehydroquinate synthase family. HAMAP MF_00110

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis HAMAP MF_00110
Cellular component	Cytoplasm HAMAP MF_00110
Ligand	NAD HAMAP MF_00110
Molecular function	Lyase HAMAP MF_00110
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-dehydroquinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	O52549 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 7DEDF6DCAA5F8347	FASTA	351	36,931
	10 20 30 40 50 60 MRTTIPVRLA ERSYDVLVGP GVRAALPEVV RRLGARRAVV VSARPADWVP GTGVETLLLQ 70 80 90 100 110 120 ARDGEPTKRL STVEELCGEF ARFGLTRSDV VVSCGGGTTT DVVGLAAALY HRGVAVVHLP 130 140 150 160 170 180 TSLLAQVDAS VGGKTAVNLP AGENLVGAYW QPSAVLCDTD YLTTLPRREV LNGLGEIARC 190 200 210 220 230 240 HFIGAPDLRG RSRPEQIAAS VTLKAGIVAQ DERDTGPRHL LNYGHTLGHA LEIATGFALR 250 260 270 280 290 300 HGEAVAIGTV FAGRLAGALG RLDQSGVDEH LAVVRHYGLP AALPADVDPA VLVRQMYRDK 310 320 330 340 350 KAITGLAFVL AGPRGAELVS DVPAPVVTDV LDRMPRDSLE NLVGTTEAAA P

References

[1]	"Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699." August P.R., Tang L., Yoon Y.J., Ning S., Muller R., Yu T.W., Taylor M., Hoffmann D., Kim C.G., Zhang X., Hutchinson C.R., Floss H.G. Chem. Biol. 5:69-79(1998) [PubMed: 9512878] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01717.1.
[2]	"3-amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics." Kim C.G., Yu T.W., Fryhle C.B., Handa S., Floss H.G. J. Biol. Chem. 273:6030-6040(1998) [PubMed: 9497318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01717.1.
[3]	"Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699." Yu T.W., Muller R., Muller M., Zhang X., Draeger G., Kim C.G., Leistner E., Floss H.G. J. Biol. Chem. 276:12546-12555(2001) [PubMed: 11278540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01717.1.
[4]	"Rifamycin insusceptibility: exploring the rif gene cluster of Amycolatopsis mediterranei S699." Yu T.-W., Pogosova-Agadjanyan E.L., Kuan L.-Y., Bai L., Tin A.M., Adman E., Floss H.G. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01717.1.
[5]	Yu T., August P.R., Tang L., Yoon Y.J., Ning S., Mueller R., Taylor M., Kim C., Zhang X., Pogosova-Agadjanyan E.L., Tin A.M., Hutchinson C.R., Floss H.G. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01717.1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF040570 Genomic DNA. Translation: AAC01717.1.
3D structure databases
HSSP	HSSP built from PDB template 1NVE based on UniProtKB P07547.
ProteinModelPortal	O52549.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00110. DHQ_synthase. [Tree]
InterPro	IPR016037. DHQ_synth_AroB. [Graphical view]
PANTHER	PTHR21090:SF1. DHQ_synth_AroB. 1 hit.
PIRSF	PIRSF001455. DHQ_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

O52549_AMYMD

Accession

Primary (citable) accession number: O52549

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 1998
Last sequence update:	June 1, 1998
Last modified:	September 21, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)