O52547 (RIFF_AMYMD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-amino-5-hydroxybenzoic acid synthase
Short name=AHBA synthase
EC=2.3.1.-

Alternative name(s):
Rifamycin amide synthase

Gene names

Name:

rifF

Organism

Amycolatopsis mediterranei (Nocardia mediterranei)

Taxonomic identifier

33910 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Amycolatopsis

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Putatively terminates polyketide biosynthesis by transfer of polyketide acyl chain to the secondary amine on the 3-AHBA starter unit.
Sequence similarities	Belongs to the arylamine N-acetyltransferase family.

Ontologies

Keywords
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Molecular function	acetyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	3-amino-5-hydroxybenzoic acid synthase		PRO_0000107920
Sites
	Active site	73	1	Acyl-thioester intermediate By similarity
	Active site	111	1	By similarity
	Active site	126	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O52547 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: C32605EBC0BFBF47	FASTA	260	29,192
	10 20 30 40 50 60 MNVFDVETYL QRIGCGGETG VDLETLAKLQ KSHLMAIPYS SLAYELRDAV NVVDLDEDDV 70 80 90 100 110 120 FVTSIAEGQG GACYHLNRLF HRLLTELGYD VTPLAGSTAE GRETFGTDVE HMFNLVTLDG 130 140 150 160 170 180 ADWLVDVGYP GPTYVEPLAV SPAVQTQYGS QFRLVEQETG YALQRRGAVT RWSVVYTFTT 190 200 210 220 230 240 QPRQWSDWKE LEDNFRALVG DTTRTDTQET LCGRAFANGQ VFLRQRRYLT VENGREQVRT 250 260 ITDDDEFRAL VSRVLSGDHG

References

[1]	"3-amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics." Kim C.G., Yu T.W., Fryhle C.B., Handa S., Floss H.G. J. Biol. Chem. 273:6030-6040(1998) [PubMed: 9497318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S699.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF040570 Genomic DNA. Translation: AAC01715.1.
3D structure databases
ProteinModelPortal	O52547.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14108.
Family and domain databases
InterPro	IPR001447. N-AcTrfase. [Graphical view]
PANTHER	PTHR11786. Acetyltransf2. 1 hit.
Pfam	PF00797. Acetyltransf_2. 1 hit. [Graphical view]
PRINTS	PR01543. ANATRNSFRASE.
ProtoNet	Search...

Entry information

Entry name

RIFF_AMYMD

Accession

Primary (citable) accession number: O52547

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 1, 1998
Last modified:	September 21, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program