O52545 (O52545_AMYMD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
RifB EMBL AAC01711.1

Gene names

Name:

rifB EMBL AAC01711.1

Organism

Amycolatopsis mediterranei (Nocardia mediterranei) EMBL AAC01711.1

Taxonomic identifier

33910 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Amycolatopsis

Protein attributes

Sequence length	5060 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Keywords
Ligand	Phosphopantetheine RuleBase RU000721
Molecular function	Transferase RuleBase RU000693
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: InterPro cofactor binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1716	1716	polyketide synthase module 4 EMBL AAC01711.1		PRO_5000053948
	Chain	35 – 461	427	ketoacyl synthase EMBL AAC01711.1		PRO_5000053949
	Chain	564 – 881	318	methylmalonyl acyltransferase EMBL AAC01711.1		PRO_5000053950
	Chain	892 – 1091	200	dehydratase EMBL AAC01711.1		PRO_5000053951
	Chain	1346 – 1590	245	ketoreductase EMBL AAC01711.1		PRO_5000053952
	Chain	1624 – 1698	75	acyl carrier protein EMBL AAC01711.1		PRO_5000053953
	Chain	1717 – 3322	1606	polyketide synthase module 5 EMBL AAC01711.1		PRO_5000053955
	Chain	1717 – 2140	424	ketoacyl synthase EMBL AAC01711.1		PRO_5000053954
	Chain	2244 – 2552	309	methylmalonyl acyltransferase EMBL AAC01711.1		PRO_5000053956
	Chain	2562 – 2724	163	dehydratase EMBL AAC01711.1		PRO_5000053957
	Chain	2954 – 3198	245	ketoreductase EMBL AAC01711.1		PRO_5000053958
	Chain	3231 – 3305	75	acyl carrier protein EMBL AAC01711.1		PRO_5000053959
	Chain	3323 – 5060	1738	polyketide synthase module 6 EMBL AAC01711.1		PRO_5000053961
	Chain	3323 – 3748	426	ketoacyl synthase EMBL AAC01711.1		PRO_5000053960
	Chain	3850 – 4165	316	methylmalonyl acyltransferase EMBL AAC01711.1		PRO_5000053962
	Chain	4176 – 4375	200	dehydratase EMBL AAC01711.1		PRO_5000053963
	Chain	4657 – 4899	243	ketoreductase EMBL AAC01711.1		PRO_5000053964
	Chain	4932 – 5006	75	acyl carrier protein EMBL AAC01711.1		PRO_5000053965

Sequences

	Sequence		Length	Mass (Da)
	O52545 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 13A6EEBE1A48A6B0	FASTA	5,060	526,557
	10 20 30 40 50 60 MSAPNEQIVD ALRASLKENV RLQQENSALA AAAAEPVAIV SMACRYAGGI RGPEDFWRVV 70 80 90 100 110 120 SEGADVYTGF PEDRGWDVEG LYHPDPDNPG TTYVREGAFL QDAAQFDAGF FGISPREALA 130 140 150 160 170 180 MDPQQRQLLE VSWETLERAG IDPHSVRGSD IGVYAGVVHQ DYAPDLSGFE GFMSLERALG 190 200 210 220 230 240 TAGGVASGRV AYTLGLEGPA VTVDTMCSSS LVAIHLAAQA LRRGECSMAL AGGSTVMATP 250 260 270 280 290 300 GGFVGFARQR ALAFDGRCKS YAAAADGSGW AEGVGVLLLE RLSVARERGH QVLAVIRGSA 310 320 330 340 350 360 VNQDGASNGL TAPNGPAQQR VIRKALASAG LTPSDVDTVE GHGTGTVLGD PIEVQALLAT 370 380 390 400 410 420 YGQGRDPQQP LWLGSVKSVV GHTQAASGVA GVIKMVQSLR HGQLPATQHV DAPTPQVDWS 430 440 450 460 470 480 AGAIELLAEG REWPRNGHPR RGGISSFGAS GTNAHMILEE APEDEPVTEA PAPTGVVPLV 490 500 510 520 530 540 VSAATAASLA AQAGRLAEVG DVSLADVAGT LVSGRAMLSE RAVVVAGSHE EAVTGLRALA 550 560 570 580 590 600 RGESAPGLLS GRGSGVPGKV VWVFPGQGTQ WAGMGRELLD SSEVFAARIA ECETALGRWV 610 620 630 640 650 660 DWSLTDVLRG EADLLDRVDV VQPASFAVMV GLAAVWASLG VEPEAVVGHS QGEIAAACVS 670 680 690 700 710 720 GALSLEDAAK VVALRSQAIA ASLAGRGGMA SVALSEEDAT ARLEPWAGRV EVAAVNGPTS 730 740 750 760 770 780 VVIAGDAEAL DEALDALDDQ GVRIRRVAVD YASHTRHVEA ARDALAEMLG GIRAQAPEVP 790 800 810 820 830 840 FYSTVTGGWV EDAGVLDGGY WYRNLRRQVR FGPAVAELIE QGHRVFVEVS AHPVLVQPIN 850 860 870 880 890 900 ELVDDTEAVV TGTLRREDGG LRRLLASAAE LFVRGVTVDW SGVLPPSRRV ELPTYAFDHQ 910 920 930 940 950 960 HYWLQMGGSA TDAVSLGLAG ADHPLLGAVV PLPQSDGLVF TSRLSLKSHP WLAGHAIGGV 970 980 990 1000 1010 1020 VLIPGTVYVD LALRAGDELG FGVLEELVIE APLVLGERGG VRVQVAVSGP NETGSRAVDV 1030 1040 1050 1060 1070 1080 FSMREDGDEW TRHATGLLGA STSREPSRFD FAAWPPAGAE PIDVENFYTD LTERGYAYSG 1090 1100 1110 1120 1130 1140 AFQGMRAVWR RGDEVFAEVA LPDDHREDAG KFGLHPALLD AALHTNAFAN PDDDRSVLPF 1150 1160 1170 1180 1190 1200 AWNGLVLHAV GASALRVRVA PGGPDALTFQ AADETGGLVV TMDSLVSREV SAAQLETAAG 1210 1220 1230 1240 1250 1260 EERDSLFQVD WIEVPATETA ATEHAEVLEA FGEAAPLELT SRVLEAVQSW LADAADEARL 1270 1280 1290 1300 1310 1320 VVVTRGAVRE VTDPAGAAVW GLVRAAQAEN PGRIILVDTD GDVPLGAVLA SGEPQLAVRG 1330 1340 1350 1360 1370 1380 NAFSVPRLAR ATGEVPEAPA VFSPEGTVLL TGGTGSLGGL VAKHLVARHG VRRLVLASRR 1390 1400 1410 1420 1430 1440 GVAAEDLVTE LTEQGATVSV VACDVSDRDQ VAALLAEHRP TGIVHLAGLL DDGVIGALNR 1450 1460 1470 1480 1490 1500 ERLAGVFAPK VDAVQHLDEL TRDLGLDAFV VFSSAAALMG SAGQGNYAAA NAFLDGLMAG 1510 1520 1530 1540 1550 1560 RRAAGLPGVS LAWGLWEQAD GLTANLSATD QARMSRGGVL PMTPAEALDI FDIGLAAEQA 1570 1580 1590 1600 1610 1620 LLVPIKLDLR TLRGQATAGG EVPHLLRGLV RASRRVTRTA AASGGGGLVH KLAGRPAEEQ 1630 1640 1650 1660 1670 1680 EAVLLGIVQA EAAAVLGFNA PELAQGTRGF SDLGFDSLTA VELRNRLSAA TGVKLPATLV 1690 1700 1710 1720 1730 1740 FDYPTPVALA RHLREELGET VAGAPATPVT TVADAGEPIA IVGMACRLPG GVMSPDDLWR 1750 1760 1770 1780 1790 1800 MVAEGRDGMS PFPGDRGWDL DGLFDSDPER PGTAYIRQGG FLHEAALFDP GFFGISPREA 1810 1820 1830 1840 1850 1860 LAMDPQQRLL LEASWEALER AGIDPTKARG DAVGVFSGVS IHDYLESLSN MPAELEGFVT 1870 1880 1890 1900 1910 1920 TATAGSVASG RVSYTFGFEG PAVTVDTACS SSLVAIHLAA QALRQGECTM ALAGGVAVMG 1930 1940 1950 1960 1970 1980 SPIGVIGMSR QRGMAEDGRV KAFADGADGT VLSEGVGIVV LERLSVARER GHRVLAVLRG 1990 2000 2010 2020 2030 2040 SAVNQDGASN GLTAPNGPSQ QRVIRSALAG AGLQPSEVDV VEAHGTGTAL GDPIEAQALL 2050 2060 2070 2080 2090 2100 ATYGKSRETP LWLGSLKSNI GHTQAAAGVA AVIKMVQALR HDTLPPTLHV QEPTKQVDWS 2110 2120 2130 2140 2150 2160 AGAVELLTEG REWARNGHPR RAGVSSFGIS GTNAHLILEE APADDTAEAD VPDAVVPVVI 2170 2180 2190 2200 2210 2220 SARSTGSLAG QAGRLAAFLD GDVPLTRVAG ALLSTRATLT DRAVVVAGSA EEARAGLTAL 2230 2240 2250 2260 2270 2280 ARGESASGLV TGTAGMPGKT VWVFPGQGTQ WAGMGRELLE ASPVFAERIE ECAAALQPWI 2290 2300 2310 2320 2330 2340 DWSLLDVLRG EGELDRVDVL QPACFAVMVG LAAVWASVGV VPDAVLGHSQ GEIAAACVSG 2350 2360 2370 2380 2390 2400 ALSLEDAAKV VALRSQAIAA ELSGRGGMAS IQLSHDEVAA RLAPWAGRVE IAAVNGPASV 2410 2420 2430 2440 2450 2460 VIAGDAEALT EAVEVLGGRR VAVDYASHTR HVEDIQDTLA ETLAGIDAQA PVVPFYSTVA 2470 2480 2490 2500 2510 2520 GEWITDAGVV DGGYWYRNLR NQVGFGPAVA ELIEQGHGVF VEVSAHPVLV QPISELTDAV 2530 2540 2550 2560 2570 2580 VTGTLRRDDG GVRRLLTSMA ELFVRGVPVD WATMAPPARV ELPTYAFDHQ HFWLSPPAVA 2590 2600 2610 2620 2630 2640 DAPALGLAGA DHPLLGAVLP LPQSDGLVFT SRLSVRTHPW LADGVPAAAL VELAVRAGDE 2650 2660 2670 2680 2690 2700 AGCPVLADLT VEKLLVLPES GGLRVQVIVS GERTVEVYSQ LEGAEDWIRN ATGHLSATAP 2710 2720 2730 2740 2750 2760 AHEAFDFTAW PPAGAQQVDG LWRRGDEIFA EVALPEELDA GAFGIHPFLL DAAVQPVLAD 2770 2780 2790 2800 2810 2820 DEQPAEWRSL VLHAAGASAL RVRLVPGGAL QAADETGGLV LTADSVAGRE LSAGKTRAGS 2830 2840 2850 2860 2870 2880 LYRVDWTEVS IADSAVPANI EVVEAFGEEP LELTGRVLEA VQTWLVTAAD DARLVVVTRG 2890 2900 2910 2920 2930 2940 AVREVTDPAG AAVWGLVRAA QAENPGRIFL IDTDGEIPAL TGDEPEIAVR GGKFFVPRIT 2950 2960 2970 2980 2990 3000 RAEPSGAAVF RPDGTVLISG AGALGGLVAR RLVERHGVRK LVLASRRGRD ADGVADLVAD 3010 3020 3030 3040 3050 3060 LAADVSVVAC DVSDRAQVAA LLDEHRPTAV VHTAGVIDAG VIETLDRDRL ATVFAPKVDA 3070 3080 3090 3100 3110 3120 VRHLDELTRD RDLDAFVVYS SVSAVFMGAG SGSYAAANAF LDGLMANRRA AGLPGLSLAW 3130 3140 3150 3160 3170 3180 GLWDQSTGMA AGTDEATRAR MSRRGGLQIM TQAEGMDLFD AALSSAESLL VPAKLDLRGV 3190 3200 3210 3220 3230 3240 RADAAAGGVV PHMLRGLVRA GRAQARAAST VDNGLAGRLA GLAPADQLTL LLDLVRAQVA 3250 3260 3270 3280 3290 3300 AVLGHADASA VRVDTAFKDA GFDSLTAVEL RNRMRTATGL KLPATLVFDY PNPQALARHL 3310 3320 3330 3340 3350 3360 RDELGGAAQT PVTTAAAKAD LDEPIAIVGM ACRLPGGVAG PEDLWRLVAE GRDAVSSFPT 3370 3380 3390 3400 3410 3420 DRGWDTDSLY DPDPARPGKT YTRHGGFLHE AGLFDAGFFG ISPREAVAMD PQQRLLLEAS 3430 3440 3450 3460 3470 3480 WEAMEDAGVD PLSLKGNDVG VFTGMFGQGY VAPGDSVVTP ELEGFAGTGG SSSVASGRVS 3490 3500 3510 3520 3530 3540 YVFGFEGPAV TIDSACSSSL VAMHLAAQSL RQGECSMALA GGATVMANPG AFVEFSRQRG 3550 3560 3570 3580 3590 3600 LAVDGRCKAF AAAADGTGWA EGVGVVILER LSVARERGHR ILAVLRGSAV NQDGASNGLT 3610 3620 3630 3640 3650 3660 APNGPSQQRV IRRALVSAGL APSDVDVVEA HGTGTTLGDP IEAQALLATY GKDRESPLWL 3670 3680 3690 3700 3710 3720 GSLKSNIGHA QAAAGVAGVI KMVQALRHEV LPPTLHVDRP TPEVDWSAGA VELLTEAREW 3730 3740 3750 3760 3770 3780 PRNGRPRRAG VSAFGVSGTN AHLILEEAPA EEPVPTPEVP LVPVVVSARS RASLAGQAGR 3790 3800 3810 3820 3830 3840 LAGFVAGDAS LAGVARALVT NRAALTERAV MVVGSREEAV TNLEALARGE DPAAVVTGRA 3850 3860 3870 3880 3890 3900 GSPGKLVWVF PGQGSQWIGM GRELLDSSPV FAERVAECAA ALEPWIDWSL LDVLRGESDL 3910 3920 3930 3940 3950 3960 LDRVDVVQPA SFAMMVGLAA VWQSVGVRPD AVVGHSQGEI AAACVSGALS LQDAAKVVAL 3970 3980 3990 4000 4010 4020 RSQAIATRLA GRGGMASVAL SEEDATAWLA PWADRVQVAA VNSPASVVIA GEAQALDEVV 4030 4040 4050 4060 4070 4080 DALSGQEVRV RRVAVDYGSH TNQVEAIEDL LAETLAGIEA QAPKVPFYST LIGDWIRDAG 4090 4100 4110 4120 4130 4140 IVDGGYWYRN LRNQVGFGPA VAELVRQGHG VFVEVSAHPV LVQPLSELSD DAVVTGSLRR 4150 4160 4170 4180 4190 4200 EDGGLRRLLT SMAELYVQGV PLDWTAVLPR TGRVDLPKYA FDHRHYWLRP AESATDAASL 4210 4220 4230 4240 4250 4260 GQGAADHPLL GAVVELPQSD GLVFTSRLSV RTHPWLADHA VGGVVILPGS GLAELAVRAG 4270 4280 4290 4300 4310 4320 DEAGCTALDE LIIEAPLVVP AQGAVRVQVA LSGPDETGSR TVDLYSQRDG GAGTWTRHAT 4330 4340 4350 4360 4370 4380 GVPSTAPAQE PEFDFHAWPP ADAERIDVET FYTDLAERGY GYGPAFQGLQ AVWRRDGDVF 4390 4400 4410 4420 4430 4440 AEVALPEDLR KDAGRFGVHP ALLDAALQAA TAVGGDEPGQ PVLAFAWNGL VLHAAGASAL 4450 4460 4470 4480 4490 4500 RVRLAPSGPD TLSVAAADET GGLVLTMESL VSRPVSAEQL GAAADAGHDA MFRVDWTELP 4510 4520 4530 4540 4550 4560 AVPRAELPPW VRIDTADDVA ALAEKADAPP VVVWEAAGGD PALAVSSRVL EIMQAWLAAP 4570 4580 4590 4600 4610 4620 AFEEARLVVT TRGAVPAGGD HTLTDPAAAA VWGLVRSAQA EHPDRVVLLD TDGEVPLGAV 4630 4640 4650 4660 4670 4680 LASGEPQLAV RGTTFFVPRL ARATRLSDAP PAFDPDGTVL VSGAGSLGTL VARHLVTRHG 4690 4700 4710 4720 4730 4740 VRRVVLASRQ GRDAEGAQDL ITELTGEGAD VSFVACDVSD RDQVAALLAG LPDLTGVVHT 4750 4760 4770 4780 4790 4800 AGVFEDGVIE ALTPDQLANV YAAKVTAAMH LDELTRDRDL GAFVVFSSVA GVMGGGGQGP 4810 4820 4830 4840 4850 4860 YAAANAFLDA AMASRQAAGL PGLSLAWGLW ERSSGMAAHL SEVDHARASR NGVLELTRAE 4870 4880 4890 4900 4910 4920 GLALFDLGLR MAESLLVPIK LDLAAMRAST VPVLFRGLVR PSRTQARTAS TVDRGLAGRL 4930 4940 4950 4960 4970 4980 AGLPVAERAA VLVDLVRGQV AVVLGYDGPE AVRPDTAFKD TGFDSLTSVE LRNRLREATG 4990 5000 5010 5020 5030 5040 LKLPATLVFD YPNPLAVARY LGARLVPDGT ANGNGNGNGH SEDDRLRHAL AAIAAEDAGE 5050 5060 ERSIADLGVD DLVQLAFGDE

References

[1]	"Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699." August P.R., Tang L., Yoon Y.J., Ning S., Muller R., Yu T.W., Taylor M., Hoffmann D., Kim C.G., Zhang X., Hutchinson C.R., Floss H.G. Chem. Biol. 5:69-79(1998) [PubMed: 9512878] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01711.1.
[2]	"3-amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics." Kim C.G., Yu T.W., Fryhle C.B., Handa S., Floss H.G. J. Biol. Chem. 273:6030-6040(1998) [PubMed: 9497318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01711.1.
[3]	"Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699." Yu T.W., Muller R., Muller M., Zhang X., Draeger G., Kim C.G., Leistner E., Floss H.G. J. Biol. Chem. 276:12546-12555(2001) [PubMed: 11278540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01711.1.
[4]	"Rifamycin insusceptibility: exploring the rif gene cluster of Amycolatopsis mediterranei S699." Yu T.-W., Pogosova-Agadjanyan E.L., Kuan L.-Y., Bai L., Tin A.M., Adman E., Floss H.G. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01711.1.
[5]	Yu T., August P.R., Tang L., Yoon Y.J., Ning S., Mueller R., Taylor M., Kim C., Zhang X., Pogosova-Agadjanyan E.L., Tin A.M., Hutchinson C.R., Floss H.G. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01711.1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF040570 Genomic DNA. Translation: AAC01711.1.
3D structure databases
HSSP	HSSP built from PDB template 1MLA based on UniProtKB P25715.
ProteinModelPortal	O52545.
SMR	O52545. Positions 34-905, 4916-5005.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001227. Ac_transferase_dom. IPR009081. Acyl_carrier_prot-like. IPR014043. Acyl_transferase. IPR016035. Acyl_Trfase/lysoPLipase. IPR000794. Beta-ketoacyl_synthase. IPR002198. DH_sc/Rdtase_SDR. IPR018201. Ketoacyl_synth_AS. IPR014031. Ketoacyl_synth_C. IPR014030. Ketoacyl_synth_N. IPR016036. Malonyl_transacylase_ACP-bd. IPR016040. NAD(P)-bd_dom. IPR006163. Phsphopanteth-bd. IPR020842. PKS/FAS_KR. IPR020801. PKS_acyl_transferase. IPR020841. PKS_Beta-ketoAc_synthase_dom. IPR020807. PKS_dehydratase. IPR013968. PKS_KR. IPR020806. PKS_PP-bd. IPR006162. PPantetheine_attach_site. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.366.10. Ac_transferase_reg. 6 hits. G3DSA:1.10.1200.10. ACP_like. 3 hits. G3DSA:3.40.50.720. NAD(P)-bd. 3 hits. G3DSA:3.40.47.10. Thiolase-like_subgr. 6 hits.
PANTHER	PTHR11712. Ketoacyl_synth. 1 hit.
Pfam	PF00698. Acyl_transf_1. 3 hits. PF00106. adh_short. 2 hits. PF00109. ketoacyl-synt. 3 hits. PF02801. Ketoacyl-synt_C. 3 hits. PF08659. KR. 1 hit. PF00550. PP-binding. 3 hits. [Graphical view]
SMART	SM00827. PKS_AT. 3 hits. SM00826. PKS_DH. 3 hits. SM00822. PKS_KR. 3 hits. SM00825. PKS_KS. 3 hits. SM00823. PKS_PP. 3 hits. [Graphical view]
SUPFAM	SSF47336. ACP_like. 3 hits. SSF52151. Acyl_Trfase/lysoPlipase. 3 hits. SSF55048. Malonyl_transacylase_ACP-bd. 3 hits. SSF53901. Thiolase-like. 3 hits.
PROSITE	PS50075. ACP_DOMAIN. 3 hits. PS00606. B_KETOACYL_SYNTHASE. 2 hits. PS00012. PHOSPHOPANTETHEINE. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

O52545_AMYMD

Accession

Primary (citable) accession number: O52545

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 1998
Last sequence update:	June 1, 1998
Last modified:	December 14, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)