Reviewed, UniProtKB/Swiss-Prot O52535 (CAH_KLEPN)

Names and origin

Protein names

Recommended name:
    Carbonic anhydrase
    EC=4.2.1.1
Alternative name(s):
    Carbonate dehydratase

Gene names

Name:

cah

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reversible hydration of carbon dioxide.
Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Zinc By similarity.
Subcellular location	Periplasm Potential.
Sequence similarities	Belongs to the alpha-carbonic anhydrase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Lyase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbonate dehydratase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 246	224	Carbonic anhydrase		PRO_0000004265
Sites
	Metal binding	111	1	Zinc; catalytic By similarity
	Metal binding	113	1	Zinc; catalytic By similarity
	Metal binding	130	1	Zinc; catalytic By similarity
Amino acid modifications
	Disulfide bond	46 ↔ 201		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O52535-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: A337BC0020A7427A	FASTA	246	27,145
	10 20 30 40 50 60 MKTSLGKAAL LALSMMPVTV FASHWSYEGE GSPEHWGALN EEYKTCQNGM NQSPINIDAT 70 80 90 100 110 120 FKTHLSPLDT HYIDGPITLI NNGHTIQAAL KTTTADTITI DGTPFILQQF HFHAPSENTV 130 140 150 160 170 180 HGKHYAMEMH LVHKNAKGAV AVVAVMFEQG AENTELNKLW ATMPEQAEQT AKIVTQMDLN 190 200 210 220 230 240 ALLPIDKTYW RFSGSLTTPP CSEGVTRIVL KHPLTLSSAQ LAKFSHAMHH DNNRPVQPLN GRVVIE

References

[1]	"Identification and characterization of the fis operon in enteric bacteria." Beach M.B., Osuna R. J. Bacteriol. 180:5932-5946(1998) [PubMed: 9811652] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF040380 Genomic DNA. Translation: AAC77887.1.
3D structure databases
SMR	O52535. Positions 23-246.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.2.1.1. 758.
Family and domain databases
InterPro	IPR001148. Carbonic_anhydrase_a-class_cat. IPR018338. Carbonic_anhydrase_a-class_CS. IPR018340. Carbonic_anhydrase_CAH1-like. [Graphical view]
Gene3D	G3DSA:3.10.200.10. Euk_COanhd. 1 hit.
PANTHER	PTHR18952:SF2. Carbonic_anhydrase_CAH1-like. 1 hit. PTHR18952. Euk_COanhd. 1 hit.
Pfam	PF00194. Carb_anhydrase. 1 hit. [Graphical view]
PROSITE	PS00162. ALPHA_CA_1. 1 hit. PS51144. ALPHA_CA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAH_KLEPN

Accession

Primary (citable) accession number: O52535

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	June 1, 1998
Last modified:	January 19, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)