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Protein

Malto-oligosyltrehalose trehalohydrolase

Gene

treZ

Organism
Brevibacterium helvolum
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan.By similarity

Pathwayi: trehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei258NucleophileBy similarity1
Active sitei295Proton donorBy similarity1
Sitei391Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BRENDAi3.2.1.141. 964.
UniPathwayiUPA00299.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Malto-oligosyltrehalose trehalohydrolase (EC:3.2.1.141By similarity)
Short name:
MTHase
Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase
Maltooligosyl trehalose trehalohydrolase
Gene namesi
Name:treZ
OrganismiBrevibacterium helvolum
Taxonomic identifieri1704 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesBrevibacteriaceaeBrevibacterium

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000543231 – 589Malto-oligosyltrehalose trehalohydrolaseAdd BLAST589

Structurei

3D structure databases

ProteinModelPortaliO52520.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni256 – 261Substrate bindingBy similarity6
Regioni320 – 324Substrate bindingBy similarity5
Regioni390 – 395Substrate bindingBy similarity6

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR022567. DUF3459.
IPR006047. Glyco_hydro_13_cat_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR012768. Trehalose_TreZ.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PF11941. DUF3459. 1 hit.
PIRSFiPIRSF006337. Trehalose_TreZ. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02402. trehalose_TreZ. 1 hit.

Sequencei

Sequence statusi: Complete.

O52520-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLVNVGPER FDVWAPDVSS VVLVADGRQY PMQKKETAPG SEGWWTASDA
60 70 80 90 100
PPNGDVDYGY LLDGNTTPVP EPRSRRLPAG VHNHSRTYNP PPYRWQDSRW
110 120 130 140 150
RGKELQGTLI YQLHVGTSTP DGTLDAAGEK LSYLVDLGID FIELLPVNGF
160 170 180 190 200
NGTHNWGYDG VQWYTVHEGY GGPAAYQRFV DAAHAAGLGV IQDVVYNHLG
210 220 230 240 250
LRGNYFPKLG PNLKQGDANT LGDSVNLDGA GSDVFREYIL DNAALWVGDY
260 270 280 290 300
HVDGVGFDAV HAVRDERAVH ILEDLGALGD AISGETGLPK TLIAESDFNN
310 320 330 340 350
PRLIYPRDVN GYGLAGQWSD DFHTAVHVSV SGETTGYYSD FESLAVLAKV
360 370 380 390 400
LKDGFLHDGS YSSFRGRHHG RPINPSLANP AALVVCNQNH DQIGNRATGD
410 420 430 440 450
RLSQSLSYGQ LAVAAVLTLT SPFTPMLFMG EEYGASTPWQ FFTSHPEPEL
460 470 480 490 500
GKATAEGRIK EFERMGWDPA VVPDPQDPET FNRSKLDWSE ASTGDHARLL
510 520 530 540 550
ELYKSLTALR REHPDLADLG FGQTEVSFDD DAGWLRFRPV SVEVLVNLSD
560 570 580
AKVRLDDAAG DLLLATDEGN PLDGGSLALV PWSAAVLKS
Length:589
Mass (Da):64,217
Last modified:June 1, 1998 - v1
Checksum:iC282314B6E9BA029
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039919 Genomic DNA. Translation: AAB95369.1.

Similar proteinsi

Entry informationi

Entry nameiTREZ_BREHE
AccessioniPrimary (citable) accession number: O52520
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: June 1, 1998
Last modified: August 30, 2017
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families