Reviewed, UniProtKB/Swiss-Prot O52513 (MTS1_STRFI)

Names and origin

Protein names

Recommended name:
    Modification methylase SfiI
      Short name=M.SfiI
    EC=2.1.1.113
Alternative name(s):
    N-4 cytosine-specific methyltransferase SfiI

Gene names

Name:

sfiIM

Organism

Streptomyces fimbriatus

Taxonomic identifier

68197 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGCCNNNNNGGCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the SfiI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation on cytosine Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Modification methylase SfiI		PRO_0000087934

Sequences

	Sequence		Length	Mass (Da)
	O52513-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 54964454A28623C1	FASTA	421	47,624
	10 20 30 40 50 60 MRKPSSGQPA GLDAWDETRS LATHAGPDFA LYVGDSLDCL AKLPDESINT VVTSPPYWAV 70 80 90 100 110 120 RDYEHDEQLG LEDEVDDYVE RLVKIFREVY RVLATDGSAW LNIGDSYFNK QITVGGKPPR 130 140 150 160 170 180 TGWKRNKQLS LVPFRVALAL QDDGWWIRNV AVWHKPNAMP ASVRDRLTVT WEPVFLLTKS 190 200 210 220 230 240 ERYYFNLDEI RVPHQTSDAI ERRRAESGTV TGKAQGKKEL RKWLNSPRHR ATIEGIKEVE 250 260 270 280 290 300 RRPNAPAAVE LASYLRTALK EKKRSIAWVA EQLDLPFERT RHYFRTDEIG SRLPPPEVWE 310 320 330 340 350 360 QLKDLLELDA TYDEAMTVEV GDNVFRNHPN GKNPGDLLSI PTAPSGANHF AVMPRKLAHF 370 380 390 400 410 420 ALKATLPMNG SCLDPFMGSG TTGRVVRELG GRFVGVDVNE HYMTDYLVES GVISPETETL W

References

[1]	"Characterization of the SfiI restriction and modification genes." van Cott E.M., Moran L.S., Slatko B.E., Wilson G.G. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF039750 Genomic DNA. Translation: AAB95366.1.
3D structure databases
SMR	O52513. Positions 30-404.
ModBase	Search...
Protein family/group databases
REBASE	3502. M.SfiI.
Enzyme and pathway databases
BRENDA	2.1.1.113. 262351.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR001091. MeTrfase_CN4. IPR017985. MeTrfase_CN4_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTS1_STRFI

Accession

Primary (citable) accession number: O52513

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	June 1, 1998
Last modified:	January 19, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)