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Protein

Modification methylase SfiI

Gene

sfiIM

Organism
Streptomyces fimbriatus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGCCNNNNNGGCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the SfiI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.113. 6011.

Protein family/group databases

REBASEi3502. M.SfiI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase SfiI (EC:2.1.1.113)
Short name:
M.SfiI
Alternative name(s):
N-4 cytosine-specific methyltransferase SfiI
Gene namesi
Name:sfiIM
OrganismiStreptomyces fimbriatus
Taxonomic identifieri68197 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879341 – 421Modification methylase SfiIAdd BLAST421

Structurei

3D structure databases

ProteinModelPortaliO52513.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR017985. MeTrfase_CN4_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00093. N4_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52513-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKPSSGQPA GLDAWDETRS LATHAGPDFA LYVGDSLDCL AKLPDESINT
60 70 80 90 100
VVTSPPYWAV RDYEHDEQLG LEDEVDDYVE RLVKIFREVY RVLATDGSAW
110 120 130 140 150
LNIGDSYFNK QITVGGKPPR TGWKRNKQLS LVPFRVALAL QDDGWWIRNV
160 170 180 190 200
AVWHKPNAMP ASVRDRLTVT WEPVFLLTKS ERYYFNLDEI RVPHQTSDAI
210 220 230 240 250
ERRRAESGTV TGKAQGKKEL RKWLNSPRHR ATIEGIKEVE RRPNAPAAVE
260 270 280 290 300
LASYLRTALK EKKRSIAWVA EQLDLPFERT RHYFRTDEIG SRLPPPEVWE
310 320 330 340 350
QLKDLLELDA TYDEAMTVEV GDNVFRNHPN GKNPGDLLSI PTAPSGANHF
360 370 380 390 400
AVMPRKLAHF ALKATLPMNG SCLDPFMGSG TTGRVVRELG GRFVGVDVNE
410 420
HYMTDYLVES GVISPETETL W
Length:421
Mass (Da):47,624
Last modified:June 1, 1998 - v1
Checksum:i54964454A28623C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039750 Genomic DNA. Translation: AAB95366.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039750 Genomic DNA. Translation: AAB95366.1.

3D structure databases

ProteinModelPortaliO52513.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3502. M.SfiI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.1.1.113. 6011.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR017985. MeTrfase_CN4_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00093. N4_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTS1_STRFI
AccessioniPrimary (citable) accession number: O52513
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: June 24, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.