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Protein

Type-2 restriction enzyme SfiI

Gene

sfiIR

Organism
Streptomyces fimbriatus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves before N-9.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1655. SfiI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme SfiI (EC:3.1.21.4)
Short name:
R.SfiI
Alternative name(s):
Endonuclease SfiI
Type II restriction enzyme SfiI
Gene namesi
Name:sfiIR
OrganismiStreptomyces fimbriatus
Taxonomic identifieri68197 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Type-2 restriction enzyme SfiIPRO_0000077366Add
BLAST

Structurei

Secondary structure

1
269
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 73Combined sources
Helixi10 – 4132Combined sources
Beta strandi45 – 473Combined sources
Helixi49 – 6214Combined sources
Beta strandi67 – 693Combined sources
Beta strandi79 – 868Combined sources
Beta strandi93 – 10513Combined sources
Beta strandi110 – 1123Combined sources
Helixi114 – 1163Combined sources
Beta strandi121 – 1244Combined sources
Turni126 – 1283Combined sources
Beta strandi130 – 1345Combined sources
Beta strandi140 – 1456Combined sources
Beta strandi147 – 16721Combined sources
Beta strandi175 – 18612Combined sources
Helixi189 – 1913Combined sources
Helixi192 – 1954Combined sources
Beta strandi203 – 2064Combined sources
Turni211 – 2144Combined sources
Beta strandi219 – 2213Combined sources
Helixi223 – 2297Combined sources
Helixi231 – 2333Combined sources
Beta strandi234 – 2385Combined sources
Beta strandi244 – 2463Combined sources
Beta strandi249 – 2535Combined sources
Beta strandi259 – 2635Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EZVX-ray2.40A/B1-269[»]
2F03X-ray3.05A/C1-269[»]
ProteinModelPortaliO52512.
SMRiO52512. Positions 1-269.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO52512.

Family & Domainsi

Family and domain databases

InterProiIPR021580. Restrct_endonuc_II_SfiI.
[Graphical view]
PfamiPF11487. RestrictionSfiI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52512-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHQDYRELSL DELESVEKQT LRTIVQALQQ YSKEAKSIFE TTAADSSGEV
60 70 80 90 100
IVLAEDITQY ALEVAETYPI NRRFAGFIDY KRVRWLPSPH GLLPQVLLVD
110 120 130 140 150
AKASTEKNRD TLQRSQLPMD AEFRNTSSGE VVTMEAGVIP HLMLQSANDG
160 170 180 190 200
VLPAVTTSIF VHFYYRELKD VEGRYRELKS IYVLSLPHAR LKQRYNPDPD
210 220 230 240 250
TSFFGAGKHS PARGEVARIR VYFDRLKEAC PWRLQELHYS ADSEYTQPRW
260
RDLNDAGHEV TKEFLFLER
Length:269
Mass (Da):31,044
Last modified:June 1, 1998 - v1
Checksum:i3C48499BAA5205EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039750 Genomic DNA. Translation: AAB95365.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039750 Genomic DNA. Translation: AAB95365.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EZVX-ray2.40A/B1-269[»]
2F03X-ray3.05A/C1-269[»]
ProteinModelPortaliO52512.
SMRiO52512. Positions 1-269.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1655. SfiI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO52512.

Family and domain databases

InterProiIPR021580. Restrct_endonuc_II_SfiI.
[Graphical view]
PfamiPF11487. RestrictionSfiI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of the SfiI restriction and modification genes."
    van Cott E.M., Moran L.S., Slatko B.E., Wilson G.G.
    Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiT2S1_STRFI
AccessioniPrimary (citable) accession number: O52512
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: November 26, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.