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Protein

Type-2 restriction enzyme SfiI

Gene

sfiIR

Organism
Streptomyces fimbriatus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves before N-9.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1655. SfiI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme SfiI (EC:3.1.21.4)
Short name:
R.SfiI
Alternative name(s):
Endonuclease SfiI
Type II restriction enzyme SfiI
Gene namesi
Name:sfiIR
OrganismiStreptomyces fimbriatus
Taxonomic identifieri68197 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773661 – 269Type-2 restriction enzyme SfiIAdd BLAST269

Structurei

Secondary structure

1269
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 7Combined sources3
Helixi10 – 41Combined sources32
Beta strandi45 – 47Combined sources3
Helixi49 – 62Combined sources14
Beta strandi67 – 69Combined sources3
Beta strandi79 – 86Combined sources8
Beta strandi93 – 105Combined sources13
Beta strandi110 – 112Combined sources3
Helixi114 – 116Combined sources3
Beta strandi121 – 124Combined sources4
Turni126 – 128Combined sources3
Beta strandi130 – 134Combined sources5
Beta strandi140 – 145Combined sources6
Beta strandi147 – 167Combined sources21
Beta strandi175 – 186Combined sources12
Helixi189 – 191Combined sources3
Helixi192 – 195Combined sources4
Beta strandi203 – 206Combined sources4
Turni211 – 214Combined sources4
Beta strandi219 – 221Combined sources3
Helixi223 – 229Combined sources7
Helixi231 – 233Combined sources3
Beta strandi234 – 238Combined sources5
Beta strandi244 – 246Combined sources3
Beta strandi249 – 253Combined sources5
Beta strandi259 – 263Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EZVX-ray2.40A/B1-269[»]
2F03X-ray3.05A/C1-269[»]
ProteinModelPortaliO52512.
SMRiO52512.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO52512.

Family & Domainsi

Family and domain databases

InterProiIPR021580. Restrct_endonuc_II_SfiI.
[Graphical view]
PfamiPF11487. RestrictionSfiI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52512-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHQDYRELSL DELESVEKQT LRTIVQALQQ YSKEAKSIFE TTAADSSGEV
60 70 80 90 100
IVLAEDITQY ALEVAETYPI NRRFAGFIDY KRVRWLPSPH GLLPQVLLVD
110 120 130 140 150
AKASTEKNRD TLQRSQLPMD AEFRNTSSGE VVTMEAGVIP HLMLQSANDG
160 170 180 190 200
VLPAVTTSIF VHFYYRELKD VEGRYRELKS IYVLSLPHAR LKQRYNPDPD
210 220 230 240 250
TSFFGAGKHS PARGEVARIR VYFDRLKEAC PWRLQELHYS ADSEYTQPRW
260
RDLNDAGHEV TKEFLFLER
Length:269
Mass (Da):31,044
Last modified:June 1, 1998 - v1
Checksum:i3C48499BAA5205EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039750 Genomic DNA. Translation: AAB95365.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039750 Genomic DNA. Translation: AAB95365.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EZVX-ray2.40A/B1-269[»]
2F03X-ray3.05A/C1-269[»]
ProteinModelPortaliO52512.
SMRiO52512.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1655. SfiI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO52512.

Family and domain databases

InterProiIPR021580. Restrct_endonuc_II_SfiI.
[Graphical view]
PfamiPF11487. RestrictionSfiI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiT2S1_STRFI
AccessioniPrimary (citable) accession number: O52512
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.