Reviewed, UniProtKB/Swiss-Prot O52512 (T2S1_STRFI)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme SfiI
      Short name=R.SfiI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme SfiI
    Endonuclease SfiI

Gene names

Name:

sfiIR

Organism

Streptomyces fimbriatus

Taxonomic identifier

68197 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves before N-9.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 269

269

Type-2 restriction enzyme SfiI

PRO_0000077366

Secondary structure

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269

Helix Strand Turn

Details...

Helix

5 – 7

3

Helix

10 – 41

32

Beta strand

45 – 47

3

Helix

49 – 62

14

Beta strand

67 – 69

3

Beta strand

79 – 86

8

Beta strand

93 – 105

13

Beta strand

110 – 112

3

Helix

114 – 116

3

Beta strand

121 – 124

4

Turn

126 – 128

3

Beta strand

130 – 134

5

Beta strand

140 – 145

6

Beta strand

147 – 167

21

Beta strand

175 – 186

12

Helix

189 – 191

3

Helix

192 – 195

4

Beta strand

203 – 206

4

Turn

211 – 214

4

Beta strand

219 – 221

3

Helix

223 – 229

7

Helix

231 – 233

3

Beta strand

234 – 238

5

Beta strand

249 – 253

5

Beta strand

259 – 263

5

Sequences

	Sequence		Length	Mass (Da)
	O52512-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 3C48499BAA5205EA	FASTA	269	31,044
	10 20 30 40 50 60 MHQDYRELSL DELESVEKQT LRTIVQALQQ YSKEAKSIFE TTAADSSGEV IVLAEDITQY 70 80 90 100 110 120 ALEVAETYPI NRRFAGFIDY KRVRWLPSPH GLLPQVLLVD AKASTEKNRD TLQRSQLPMD 130 140 150 160 170 180 AEFRNTSSGE VVTMEAGVIP HLMLQSANDG VLPAVTTSIF VHFYYRELKD VEGRYRELKS 190 200 210 220 230 240 IYVLSLPHAR LKQRYNPDPD TSFFGAGKHS PARGEVARIR VYFDRLKEAC PWRLQELHYS 250 260 ADSEYTQPRW RDLNDAGHEV TKEFLFLER

References

[1]	"Characterization of the SfiI restriction and modification genes." van Cott E.M., Moran L.S., Slatko B.E., Wilson G.G. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF039750 Genomic DNA. Translation: AAB95365.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EZV	X-ray	2.40	A/B	1-269	[»]
2F03	X-ray	3.05	A/C	1-269	[»]

ModBase

Search...

Protein family/group databases

REBASE

1655. SfiI.

Enzyme and pathway databases

BRENDA

3.1.21.4. 262351.

Family and domain databases

ProtoNet

Search...

Entry information

Entry name

T2S1_STRFI

Accession

Primary (citable) accession number: O52512

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	June 1, 1998
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)