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O52512

- T2S1_STRFI

UniProt

O52512 - T2S1_STRFI

Protein

Type-2 restriction enzyme SfiI

Gene

sfiIR

Organism
Streptomyces fimbriatus
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 47 (16 Apr 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves before N-9.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1655. SfiI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme SfiI (EC:3.1.21.4)
    Short name:
    R.SfiI
    Alternative name(s):
    Endonuclease SfiI
    Type II restriction enzyme SfiI
    Gene namesi
    Name:sfiIR
    OrganismiStreptomyces fimbriatus
    Taxonomic identifieri68197 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 269269Type-2 restriction enzyme SfiIPRO_0000077366Add
    BLAST

    Structurei

    Secondary structure

    1
    269
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 73
    Helixi10 – 4132
    Beta strandi45 – 473
    Helixi49 – 6214
    Beta strandi67 – 693
    Beta strandi79 – 868
    Beta strandi93 – 10513
    Beta strandi110 – 1123
    Helixi114 – 1163
    Beta strandi121 – 1244
    Turni126 – 1283
    Beta strandi130 – 1345
    Beta strandi140 – 1456
    Beta strandi147 – 16721
    Beta strandi175 – 18612
    Helixi189 – 1913
    Helixi192 – 1954
    Beta strandi203 – 2064
    Turni211 – 2144
    Beta strandi219 – 2213
    Helixi223 – 2297
    Helixi231 – 2333
    Beta strandi234 – 2385
    Beta strandi244 – 2463
    Beta strandi249 – 2535
    Beta strandi259 – 2635

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2EZVX-ray2.40A/B1-269[»]
    2F03X-ray3.05A/C1-269[»]
    ProteinModelPortaliO52512.
    SMRiO52512. Positions 1-269.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO52512.

    Family & Domainsi

    Family and domain databases

    InterProiIPR021580. Restrct_endonuc_II_SfiI.
    [Graphical view]
    PfamiPF11487. RestrictionSfiI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O52512-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHQDYRELSL DELESVEKQT LRTIVQALQQ YSKEAKSIFE TTAADSSGEV    50
    IVLAEDITQY ALEVAETYPI NRRFAGFIDY KRVRWLPSPH GLLPQVLLVD 100
    AKASTEKNRD TLQRSQLPMD AEFRNTSSGE VVTMEAGVIP HLMLQSANDG 150
    VLPAVTTSIF VHFYYRELKD VEGRYRELKS IYVLSLPHAR LKQRYNPDPD 200
    TSFFGAGKHS PARGEVARIR VYFDRLKEAC PWRLQELHYS ADSEYTQPRW 250
    RDLNDAGHEV TKEFLFLER 269
    Length:269
    Mass (Da):31,044
    Last modified:June 1, 1998 - v1
    Checksum:i3C48499BAA5205EA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF039750 Genomic DNA. Translation: AAB95365.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF039750 Genomic DNA. Translation: AAB95365.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2EZV X-ray 2.40 A/B 1-269 [» ]
    2F03 X-ray 3.05 A/C 1-269 [» ]
    ProteinModelPortali O52512.
    SMRi O52512. Positions 1-269.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1655. SfiI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei O52512.

    Family and domain databases

    InterProi IPR021580. Restrct_endonuc_II_SfiI.
    [Graphical view ]
    Pfami PF11487. RestrictionSfiI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the SfiI restriction and modification genes."
      van Cott E.M., Moran L.S., Slatko B.E., Wilson G.G.
      Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiT2S1_STRFI
    AccessioniPrimary (citable) accession number: O52512
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: June 1, 1998
    Last modified: April 16, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3