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O52512 (T2S1_STRFI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme SfiI

Short name=R.SfiI
EC=3.1.21.4
Alternative name(s):
Endonuclease SfiI
Type II restriction enzyme SfiI
Gene names
Name:sfiIR
OrganismStreptomyces fimbriatus
Taxonomic identifier68197 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves before N-9.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 269269Type-2 restriction enzyme SfiI
PRO_0000077366

Secondary structure

................................................... 269
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O52512 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 3C48499BAA5205EA

FASTA26931,044
        10         20         30         40         50         60 
MHQDYRELSL DELESVEKQT LRTIVQALQQ YSKEAKSIFE TTAADSSGEV IVLAEDITQY 

        70         80         90        100        110        120 
ALEVAETYPI NRRFAGFIDY KRVRWLPSPH GLLPQVLLVD AKASTEKNRD TLQRSQLPMD 

       130        140        150        160        170        180 
AEFRNTSSGE VVTMEAGVIP HLMLQSANDG VLPAVTTSIF VHFYYRELKD VEGRYRELKS 

       190        200        210        220        230        240 
IYVLSLPHAR LKQRYNPDPD TSFFGAGKHS PARGEVARIR VYFDRLKEAC PWRLQELHYS 

       250        260 
ADSEYTQPRW RDLNDAGHEV TKEFLFLER 

« Hide

References

[1]"Characterization of the SfiI restriction and modification genes."
van Cott E.M., Moran L.S., Slatko B.E., Wilson G.G.
Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF039750 Genomic DNA. Translation: AAB95365.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EZVX-ray2.40A/B1-269[»]
2F03X-ray3.05A/C1-269[»]
ProteinModelPortalO52512.
SMRO52512. Positions 1-269.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1655. SfiI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR021580. Restrct_endonuc_II_SfiI.
[Graphical view]
PfamPF11487. RestrictionSfiI. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO52512.

Entry information

Entry nameT2S1_STRFI
AccessionPrimary (citable) accession number: O52512
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references