SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O52512

- T2S1_STRFI

UniProt

O52512 - T2S1_STRFI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Type-2 restriction enzyme SfiI

Gene
sfiIR
Organism
Streptomyces fimbriatus
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves before N-9.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1655. SfiI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme SfiI (EC:3.1.21.4)
Short name:
R.SfiI
Alternative name(s):
Endonuclease SfiI
Type II restriction enzyme SfiI
Gene namesi
Name:sfiIR
OrganismiStreptomyces fimbriatus
Taxonomic identifieri68197 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Type-2 restriction enzyme SfiIPRO_0000077366Add
BLAST

Structurei

Secondary structure

1
269
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 73
Helixi10 – 4132
Beta strandi45 – 473
Helixi49 – 6214
Beta strandi67 – 693
Beta strandi79 – 868
Beta strandi93 – 10513
Beta strandi110 – 1123
Helixi114 – 1163
Beta strandi121 – 1244
Turni126 – 1283
Beta strandi130 – 1345
Beta strandi140 – 1456
Beta strandi147 – 16721
Beta strandi175 – 18612
Helixi189 – 1913
Helixi192 – 1954
Beta strandi203 – 2064
Turni211 – 2144
Beta strandi219 – 2213
Helixi223 – 2297
Helixi231 – 2333
Beta strandi234 – 2385
Beta strandi244 – 2463
Beta strandi249 – 2535
Beta strandi259 – 2635

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EZVX-ray2.40A/B1-269[»]
2F03X-ray3.05A/C1-269[»]
ProteinModelPortaliO52512.
SMRiO52512. Positions 1-269.

Miscellaneous databases

EvolutionaryTraceiO52512.

Family & Domainsi

Family and domain databases

InterProiIPR021580. Restrct_endonuc_II_SfiI.
[Graphical view]
PfamiPF11487. RestrictionSfiI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52512-1 [UniParc]FASTAAdd to Basket

« Hide

MHQDYRELSL DELESVEKQT LRTIVQALQQ YSKEAKSIFE TTAADSSGEV    50
IVLAEDITQY ALEVAETYPI NRRFAGFIDY KRVRWLPSPH GLLPQVLLVD 100
AKASTEKNRD TLQRSQLPMD AEFRNTSSGE VVTMEAGVIP HLMLQSANDG 150
VLPAVTTSIF VHFYYRELKD VEGRYRELKS IYVLSLPHAR LKQRYNPDPD 200
TSFFGAGKHS PARGEVARIR VYFDRLKEAC PWRLQELHYS ADSEYTQPRW 250
RDLNDAGHEV TKEFLFLER 269
Length:269
Mass (Da):31,044
Last modified:June 1, 1998 - v1
Checksum:i3C48499BAA5205EA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF039750 Genomic DNA. Translation: AAB95365.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF039750 Genomic DNA. Translation: AAB95365.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2EZV X-ray 2.40 A/B 1-269 [» ]
2F03 X-ray 3.05 A/C 1-269 [» ]
ProteinModelPortali O52512.
SMRi O52512. Positions 1-269.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1655. SfiI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei O52512.

Family and domain databases

InterProi IPR021580. Restrct_endonuc_II_SfiI.
[Graphical view ]
Pfami PF11487. RestrictionSfiI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of the SfiI restriction and modification genes."
    van Cott E.M., Moran L.S., Slatko B.E., Wilson G.G.
    Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiT2S1_STRFI
AccessioniPrimary (citable) accession number: O52512
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3