Reviewed, UniProtKB/Swiss-Prot O52485 (PUTA_KLEAE)

Names and origin

Protein names

Recommended name:
    Bifunctional protein putA
Including the following 2 domains:
    1- Recommended name:
            Proline dehydrogenase
              EC=1.5.99.8
        Alternative name(s):
            Proline oxidase
    2- Recommended name:
            Delta-1-pyrroline-5-carboxylate dehydrogenase
                Short name=P5C dehydrogenase
              EC=1.5.1.12

Gene names

Name:

putA

Organism

Klebsiella aerogenes

Taxonomic identifier

28451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	1312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidizes proline to glutamate for use as a carbon and nitrogen source and also function as a transcriptional repressor of the put operon By similarity.
Catalytic activity	L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor. (S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H.
Cofactor	FAD.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2. Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.
Sequence similarities	In the N-terminal section; belongs to the proline dehydrogenase family. In the C-terminal section; belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Proline metabolism Transcription Transcription regulation
Ligand	DNA-binding FAD Flavoprotein NAD
Molecular function	Oxidoreductase Repressor
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro proline catabolic process Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: UniProtKB-KW proline dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1312	1312	Bifunctional protein putA		PRO_0000056525
Regions
	Region	228 – 574	347	Proline dehydrogenase
	Region	653 – 1119	467	Aldehyde dehydrogenase
Sites
	Active site	883	1	By similarity
	Active site	917	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O52485-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: BAF3CA7FC5D18599	FASTA	1,312	143,857
	10 20 30 40 50 60 MGTTTMGVKL DDATRERIKS AASRIDRTPH WLIKQAIFNY LEKLENDETL PELPALLSGA 70 80 90 100 110 120 ANESDDASEP TEEPYQPFLE FAEQILPQSV RRAAITAAWR RPETDAVPML LEQARLPQPL 130 140 150 160 170 180 GEQAHKLAYQ LAEKLRNQKT ASGRAGMVQS LLQEFSLSSQ EGVALMCLAE ALLRIPDKAT 190 200 210 220 230 240 RDALIRDKIS NGNWQSHIGR SPSLFVNAAT WGLLFTGKLV STHNETSLSR SLNRIIGKSG 250 260 270 280 290 300 EPLIRKGVDM AMRLMGEQFV TGETIAEALA NARKLEEKGF RYSYDMLGEA ALTAADAQAY 310 320 330 340 350 360 MVSYQQAIHA IGKASNGRGI YEGPGISIKL SALHPRYSRA QYDRVMEELY PRLKSLTLLA 370 380 390 400 410 420 RQYDIGINID AEEADRLEIS LDLLEKLCFE PELAGWNGIG FVIQAYQKRC PFVIDYLIDL 430 440 450 460 470 480 ATRSRRRLMI RLVKGAYWDS EIKRAQMEGL EGYPVYTRKV YTDVSYLACA KKLLAVPNLI 490 500 510 520 530 540 YPQFATHNAH TLAAIYQLAG QNYYPGQYEF QCLHGMGEPL YEQVVGKVAD GKLNRPCRIY 550 560 570 580 590 600 APVGTHETLL AYLVRRLLEN GANTSFVNRI ADNTLPLDEL VADPVSAVEK LAQQEGQAGL 610 620 630 640 650 660 PHPKIPLPRD LYGSGRSNSA GLDLANEHRL ASLSSSLLNS ALHKWQALPM LEQPVAEGEM 670 680 690 700 710 720 QPVVNPAEPK DIVGYVREAS DAEVQQALTS AINNAPIWFA TPPQERAAIL ERAAVLMESQ 730 740 750 760 770 780 MPTLMGILVR EAGKTFSNAI AEVREAVDFL HYYAGQVRDD FDNETHRPLG PVVCISPWNF 790 800 810 820 830 840 PLAIFTGQIA AALAAGNSVL AKPAEQTPLI AAQGVAILLE AGVPPGVIQL LPGRGETVGA 850 860 870 880 890 900 ALTSDERVRG VMFTGSTEVA TLLQRNIASR LDPQGRPTPL IAETGGMNAM IVDSSALTEQ 910 920 930 940 950 960 VVIDVLASAF DSAGQRCSAL RVLCLQEEVA DHTLTMLRGA MSECRMGNPG RLTTDIGPVI 970 980 990 1000 1010 1020 DAEAKENIER HIQAMRAKGR TVYQAVRENS EDAREWRHGT FVPPTLIELD SFDELKKEVF 1030 1040 1050 1060 1070 1080 GPVLHVVRYN RNELDKLVEQ INASGYGLTL GVHTRIDETI AQVTGSAKVG NLYVNRNMVG 1090 1100 1110 1120 1130 1140 AVVGVQPFGG EGLSGTGPKA GGPLYLYRLL SSRPQDAVGV TFARQDAERP LDAQLKTLLE 1150 1160 1170 1180 1190 1200 KPLQALQQWA AGRPELQALC QQYSEQAQSG TQRLLPGPTG ERNTLTLMPR ERVLCVADNE 1210 1220 1230 1240 1250 1260 QDALIQLAAV LAVGCEVLWP DSALQRDLAK KLPREVSERI RFAKAEQLPV QAFDAVIYHG 1270 1280 1290 1300 1310 DSDQLRELCE QVAARDGAIV SVQGFARGET NLLLERLYIE RSLSVNTAAA GA

References

[1]	"DNA sequence analysis of the putA gene in Klebsiella aerogenes." Surber M.W., Maloy S. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EM450.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF038838 Genomic DNA. Translation: AAB95478.1.
3D structure databases
SMR	O52485. Positions 2-49, 87-612, 655-1113.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.5.1.12. 366. 1.5.99.8. 366.
Family and domain databases
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR005933. d-1-pyrroline-5-COlate_DH-3. IPR002872. Proline_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. PF01619. Pro_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01238. D1pyr5carbox3. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUTA_KLEAE

Accession

Primary (citable) accession number: O52485

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	February 9, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)