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Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Edwardsiella ictaluri (strain 93-146)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62Glyceraldehyde 3-phosphateBy similarity1
Active sitei109Proton donorBy similarity1
Metal bindingi110Zinc 1; catalyticBy similarity1
Metal bindingi144Zinc 2By similarity1
Metal bindingi174Zinc 2By similarity1
Metal bindingi226Zinc 1; catalyticBy similarity1
Binding sitei227Dihydroxyacetone phosphate; via amide nitrogenBy similarity1
Metal bindingi264Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

SABIO-RKO52402.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fba
Synonyms:fda
Ordered Locus Names:NT01EI_3367
OrganismiEdwardsiella ictaluri (strain 93-146)
Taxonomic identifieri634503 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesHafniaceaeEdwardsiella
Proteomesi
  • UP000001485 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001787151 – 358Fructose-bisphosphate aldolaseAdd BLAST358

Proteomic databases

PRIDEiO52402.

Interactioni

Protein-protein interaction databases

STRINGi634503.NT01EI_3367.

Structurei

3D structure databases

ProteinModelPortaliO52402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 267Dihydroxyacetone phosphate bindingBy similarity3
Regioni286 – 289Dihydroxyacetone phosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D2N. Bacteria.
COG0191. LUCA.
HOGENOMiHOG000227794.
KOiK01624.
OMAiRMSKMGM.
OrthoDBiPOG091H01YW.

Family and domain databases

CDDicd00946. FBP_aldolase_IIA. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR006411. Fruct_bisP_bact.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52402-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIFDFVKP GVIFGDDVQK VFQVAKENKF ALPAVNCVGT DSINAVMEAA
60 70 80 90 100
AKVRAPIIVQ FSNGGAAFIA GKGLKLEGQQ GAILGAIAGA HHVHQMAEYY
110 120 130 140 150
GVPVILHTDH CAKKLLPWLD GLLDAGEKHF AATGKPLFSS HMIDLSEESL
160 170 180 190 200
EENIEICSQY LARMSKIGMT LELELGCTGG EEDGVDNSHL DNSALYTQPE
210 220 230 240 250
DVDYAFTKLS AISPRFTIAA SFGNVHGVYK PGNVQLTPVI LKNSQEYVSK
260 270 280 290 300
KHNLPHNSLN FVFHGGSGST AAEIKEAVSY GVVKMNIDTD TQWATWEGVL
310 320 330 340 350
KYYKKNEGYL QGQLGNPEGD DKPNKKYYDP RVWLRAAQTG MIERLEQAFK

ELNCIDVL
Length:358
Mass (Da):39,155
Last modified:September 1, 2009 - v2
Checksum:i0EAADF89554D8049
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20K → E in AAB92572 (PubMed:12542086).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037440 Genomic DNA. Translation: AAB92572.1.
CP001600 Genomic DNA. Translation: ACR70504.1.
RefSeqiWP_015872577.1. NC_012779.2.

Genome annotation databases

EnsemblBacteriaiACR70504; ACR70504; NT01EI_3367.
GeneIDi7959666.
KEGGieic:NT01EI_3367.
PATRICi21839466. VBIEdwIct114273_2993.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037440 Genomic DNA. Translation: AAB92572.1.
CP001600 Genomic DNA. Translation: ACR70504.1.
RefSeqiWP_015872577.1. NC_012779.2.

3D structure databases

ProteinModelPortaliO52402.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi634503.NT01EI_3367.

Proteomic databases

PRIDEiO52402.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR70504; ACR70504; NT01EI_3367.
GeneIDi7959666.
KEGGieic:NT01EI_3367.
PATRICi21839466. VBIEdwIct114273_2993.

Phylogenomic databases

eggNOGiENOG4105D2N. Bacteria.
COG0191. LUCA.
HOGENOMiHOG000227794.
KOiK01624.
OMAiRMSKMGM.
OrthoDBiPOG091H01YW.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
SABIO-RKO52402.

Family and domain databases

CDDicd00946. FBP_aldolase_IIA. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR006411. Fruct_bisP_bact.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALF_EDWI9
AccessioniPrimary (citable) accession number: O52402
Secondary accession number(s): C5BAU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: September 1, 2009
Last modified: November 30, 2016
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.