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Protein

Salicylate 5-hydroxylase, large oxygenase component

Gene

nagG

Organism
Ralstonia sp.
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Oxygenase component of the salicylate 5-hydroxylase (S5H) multicomponent enzyme system which catalyzes the 5-hydroxylation of salicylate to gentisate. Active only on substrates with a ring-substituted carboxylate group with an adjacent hydroxyl group. Primarily active against salicylate and substituted salicylates, but not against 2-hydroxycinnamate, 3-hydroxycinnamate, 2-hydroxyphenylacetate, 3-hydroxyphenylacetate, 2-hydroxybenzophenone, 1-hydroxy-2-naphthoate, 4-methoxysalicylate or 2-hydroxyacetophenone.2 Publications

Catalytic activityi

Salicylate + NADH + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • [2Fe-2S] clusterPROSITE-ProRule annotationNote: Binds 1 [2Fe-2S] cluster per subunit.PROSITE-ProRule annotation

Pathwayi: naphthalene degradation

This protein is involved in the pathway naphthalene degradation, which is part of Aromatic compound metabolism.2 Publications
View all proteins of this organism that are known to be involved in the pathway naphthalene degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi91 – 911Iron-sulfur (2Fe-2S)PROSITE-ProRule annotationBy similarity
Metal bindingi93 – 931Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotationBy similarity
Metal bindingi111 – 1111Iron-sulfur (2Fe-2S)PROSITE-ProRule annotationBy similarity
Metal bindingi114 – 1141Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotationBy similarity
Metal bindingi224 – 2241IronBy similarity
Metal bindingi229 – 2291IronBy similarity
Metal bindingi370 – 3701IronBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14766.
BRENDAi1.14.13.172. 5085.
UniPathwayiUPA00082.

Names & Taxonomyi

Protein namesi
Recommended name:
Salicylate 5-hydroxylase, large oxygenase component (EC:1.14.13.172)
Short name:
S5H large oxygenase component
Alternative name(s):
Salicylate 5-hydroxylase, oxygenase component NagG
Salicylate 5-monooxygenase, hydroxylase large subunit
Gene namesi
Name:nagGImported
Encoded oniPlasmid pWWU2Imported
OrganismiRalstonia sp.
Taxonomic identifieri54061 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are not able to convert salicylate to gentisate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Salicylate 5-hydroxylase, large oxygenase componentPRO_0000421808Add
BLAST

Interactioni

Subunit structurei

The salicylate 5-hydroxylase (S5H) multicomponent enzyme system is composed of an electron transfer component and an oxygenase component. The electron transfer component is comprised of a ferredoxin reductase (NagAa) and a ferredoxin (NagAb), and the oxygenase component is formed by a large subunit (NagG) and a small subunit (NagH).2 Publications

Structurei

3D structure databases

ProteinModelPortaliO52379.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 168120RieskePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK18242.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.90.380.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSiPR00090. RNGDIOXGNASE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
PS00570. RING_HYDROXYL_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52379-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPQRLKPV FPQDPKWPGE GSSRVPFWAY TREDLYKREL ERLFYANHWC
60 70 80 90 100
YVGLEAEIPN PGDFKRTVIG ERSVIMVRDP DGGINVVENV CAHRGMRFCR
110 120 130 140 150
ERHGNAKDFF CPYHQWNYSL KGDLQGVPFR RGVKQDGKVN GGMPKDFKLE
160 170 180 190 200
EHGLTKLKVA ARGGAVFASF DHDVEPFEEF LGPTILHYFD RVFNGRKLKI
210 220 230 240 250
LGYRRQRIPG NWKLMQENIK DPYHPGLLHT WFSTFGLWRA DNKSELKMDA
260 270 280 290 300
KFRHAAMIST RGQGGKNEEV VSGVDSFKEQ MKVNDPRLLD IVPEPWWGGP
310 320 330 340 350
TAVMTTIFPS VIIQQQVNSV STRHIQPNGH GSFDFVWTHF GFEDDNEEWT
360 370 380 390 400
QRRLIQANLF GPAGFVSADD GEVIEWSQEG FEQKPTHRTV IEMGGHEIGD
410 420
TDHMVTETLI RGMYDYWRKV MGE
Length:423
Mass (Da):48,795
Last modified:June 1, 1998 - v1
Checksum:i2EDC17B117974DC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036940 Genomic DNA. Translation: AAD12607.1.

Genome annotation databases

KEGGiag:AAD12607.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036940 Genomic DNA. Translation: AAD12607.1.

3D structure databases

ProteinModelPortaliO52379.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAD12607.

Phylogenomic databases

KOiK18242.

Enzyme and pathway databases

UniPathwayiUPA00082.
BioCyciMetaCyc:MONOMER-14766.
BRENDAi1.14.13.172. 5085.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.90.380.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSiPR00090. RNGDIOXGNASE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
PS00570. RING_HYDROXYL_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A gene cluster encoding steps in conversion of naphthalene to gentisate in Pseudomonas sp. strain U2."
    Fuenmayor S.L., Wild M., Boyes A.L., Williams P.A.
    J. Bacteriol. 180:2522-2530(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PATHWAY, DISRUPTION PHENOTYPE, SUBUNIT.
    Strain: U2Imported.
  2. "Salicylate 5-hydroxylase from Ralstonia sp. strain U2: a monooxygenase with close relationships to and shared electron transport proteins with naphthalene dioxygenase."
    Zhou N.Y., Al-Dulayymi J., Baird M.S., Williams P.A.
    J. Bacteriol. 184:1547-1555(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, PATHWAY, SUBUNIT.
    Strain: U21 Publication.

Entry informationi

Entry nameiNAGG_RALSP
AccessioniPrimary (citable) accession number: O52379
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: June 1, 1998
Last modified: May 11, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.