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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei94SubstrateBy similarity1
Binding sitei126NAD or substrateBy similarity1
Binding sitei157SubstrateBy similarity1
Active sitei181Proton acceptorBy similarity1
Binding sitei234SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 38NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMGAL710127:GC09-74-MONOMER.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase (EC:1.1.1.27)
Short name:
L-LDH
Gene namesi
Name:ldh
Synonyms:mdh
Ordered Locus Names:MYCGA0730
ORF Names:MGA_0746
OrganismiMycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Taxonomic identifieri710127 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000001418 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683671 – 323L-lactate dehydrogenaseAdd BLAST323

Proteomic databases

PRIDEiO52354.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO52354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

HOGENOMiHOG000213793.
KOiK00016.
OMAiITHYLQN.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.

Sequencei

Sequence statusi: Complete.

O52354-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIAVIGCG FVGSTYILDL LQQGVQADYL LVDKNTNLAD GHVRDLRDSK
60 70 80 90 100
SLKSHNGSTF NVGTYDDLKD ADVVAITASI PTVPTADGEV FTDRLQLMTA
110 120 130 140 150
NVKILNEIAL ELKRVGFKGL SIIPTNPCDV MAGVYQKVTG FDPHKIISTG
160 170 180 190 200
CQLETMRTRK MISEALGVNS DSVEGFVVGE HGSGAIVPWS VFRVGNVPMK
210 220 230 240 250
QLIAEGKIKE EYVKDIFSRV VKEAFEIIKF KKATYFGIAE SMSLITRAYI
260 270 280 290 300
YNLNTVLGVG VQLDDKYVAS GIYFTVPAVV GKHGWKLHSK LQLSQEEQAA
310 320
FDKSALNIQK VTKDALDLIG FKN
Length:323
Mass (Da):35,372
Last modified:August 15, 2003 - v2
Checksum:i6831D412F214C5E3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti218S → P in AAB95409 (PubMed:10867916).Curated1
Sequence conflicti255T → M in AAB95409 (PubMed:10867916).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036708 Genomic DNA. Translation: AAB95409.1.
AE015450 Genomic DNA. Translation: AAP56423.1.
RefSeqiWP_011113302.1. NC_004829.2.

Genome annotation databases

EnsemblBacteriaiAAP56423; AAP56423; MGA_0746.
GeneIDi1090122.
KEGGimga:MGA_0746.
PATRICi20007818. VBIMycGal115357_0077.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036708 Genomic DNA. Translation: AAB95409.1.
AE015450 Genomic DNA. Translation: AAP56423.1.
RefSeqiWP_011113302.1. NC_004829.2.

3D structure databases

ProteinModelPortaliO52354.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO52354.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP56423; AAP56423; MGA_0746.
GeneIDi1090122.
KEGGimga:MGA_0746.
PATRICi20007818. VBIMycGal115357_0077.

Phylogenomic databases

HOGENOMiHOG000213793.
KOiK00016.
OMAiITHYLQN.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
BioCyciMGAL710127:GC09-74-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLDH_MYCGA
AccessioniPrimary (citable) accession number: O52354
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 15, 2003
Last modified: October 5, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.