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O52353 (AMPM_MYCGA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine aminopeptidase
Short name=MAP
EC=3.4.11.18
Alternative name(s):
Peptidase M
Gene names
Name:map
Ordered Locus Names:MYCGA0720
ORF Names:MGA_0745
OrganismMycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) [Complete proteome] [HAMAP]
Taxonomic identifier710127 [NCBI]
Taxonomic lineageBacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Removes the amino-terminal methionine from nascent proteins.

Catalytic activity

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.

Cofactor

Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.

Sequence similarities

Belongs to the peptidase M24A family.

Ontologies

Keywords
   LigandCobalt
Metal-binding
   Molecular functionAminopeptidase
Hydrolase
Protease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcellular process

Inferred from electronic annotation. Source: InterPro

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionaminopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloexopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 250250Methionine aminopeptidase
PRO_0000148945

Sites

Metal binding941Cobalt 1 By similarity
Metal binding1051Cobalt 1 By similarity
Metal binding1051Cobalt 2 By similarity
Metal binding1691Cobalt 2 By similarity
Metal binding2021Cobalt 2 By similarity
Metal binding2351Cobalt 1 By similarity
Metal binding2351Cobalt 2 By similarity
Binding site771Substrate By similarity
Binding site1761Substrate By similarity

Experimental info

Sequence conflict731Q → K in AAB95408. Ref.1
Sequence conflict831E → D in AAB95408. Ref.1
Sequence conflict1171P → L in AAB95408. Ref.1
Sequence conflict1321E → D in AAB95408. Ref.1
Sequence conflict1581Y → H in AAB95408. Ref.1
Sequence conflict1611S → N in AAB95408. Ref.1
Sequence conflict189 – 1902SS → PG in AAB95408. Ref.1
Sequence conflict2171D → V in AAB95408. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O52353 [UniParc].

Last modified August 15, 2003. Version 2.
Checksum: F7EE9B91D4779F76

FASTA25029,026
        10         20         30         40         50         60 
MIYIKNPNEI QKIKNAAQIY KKIVKQFNFD YIKNKSLKEI DQMLRDFVSQ HHANSCYHGY 

        70         80         90        100        110        120 
LGFKGYHCLS LNQTIIHGLA NDEIFTSKDK LTIDIGIELD NYYCDSAFTI LGPDVNPRQK 

       130        140        150        160        170        180 
LLSEVTHNCI FELVKKIVPN QTTTNDLGIW TEEYAKKYGY SVIKDFGGHG CGIKIHEDPI 

       190        200        210        220        230        240 
ILNYGTKKSS ELLTPNMVIC IEPMFFEKDN RYYIDPDDSW SVKPVNKNQY VCHWEHMVLI 

       250 
KEDQAEILTL

« Hide

References

« Hide 'large scale' references
[1]"Determination and analysis of the nucleotide sequence of a segment of a Mycoplasma gallisepticum strain A5969 chromosome, containing operons S10 and rrn23-5."
Skamrov A.V., Gol'dman M.A., Feoktistova E.S., Bibilashvili R.S.
Mol. Biol. (Mosk.) 34:390-396(2000) [PubMed: 10867916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: A5969Var.B.
[2]"The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
Microbiology 149:2307-2316(2003) [PubMed: 12949158] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: R(low / passage 15 / clone 2).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF036708 Genomic DNA. Translation: AAB95408.1.
AE015450 Genomic DNA. Translation: AAP56422.1.
RefSeqNP_852854.1. NC_004829.2.

3D structure databases

ProteinModelPortalO52353.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1090121.
GenomeReviewsGene locus MYCGA0720 in contig AE015450_GR.
KEGGmga:MGA_0745.
PATRIC20007816. VBIMycGal115357_0076.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG299384.
OMALCHAYMR.
PhylomeDBO52353.

Family and domain databases

InterProIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view]
Gene3DG3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
KOK01265.
PfamPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSPR00599. MAPEPTIDASE.
SUPFAMSSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMsTIGR00500. Met_pdase_I. 1 hit.
PROSITEPS00680. MAP_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMPM_MYCGA
AccessionPrimary (citable) accession number: O52353
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 15, 2003
Last modified: January 25, 2012
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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