Reviewed, UniProtKB/Swiss-Prot O52310 (DHE3_PYRHO)

Names and origin

Protein names

Recommended name:
    Glutamate dehydrogenase
      Short name=GDH
    EC=1.4.1.3

Gene names

Name:	gdhA
Synonyms:	gdh
Ordered Locus Names:	PH1593

Organism

Pyrococcus horikoshii [Complete proteome] [HAMAP]

Taxonomic identifier

53953 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.
Subunit structure	Homohexamer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Glutamate dehydrogenase		PRO_0000182758
Regions
	Nucleotide binding	220 – 226	7	NAD Potential
Sites
	Active site	105	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O52310-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 1198BEC2681B5AA2	FASTA	420	47,014
	10 20 30 40 50 60 MVEQDPFEIA VKQLERAAQH MKISEEALEF LKRPQRIVEV TIPVEMDDGS VKVFTGFRVQ 70 80 90 100 110 120 YNWARGPTKG GIRWHPEETL STVKALAAWM TWKTAVMDLP YGGGKGGIIV DPKKLSDREK 130 140 150 160 170 180 ERLARGYIRA VYDIISPYED IPAPDVYTNP QIMAWMMDEY ETIARRKTPA FGIITGKPLS 190 200 210 220 230 240 IGGSLGRNEA TARGASYTIR EAAKVLGWDG LKGKTIAIQG YGNAGYYLAK IMSEDYGMKV 250 260 270 280 290 300 VAVSDSKGGI YNPDGLNADE VLKWKREHGS VKDFPGATNI SNEELLELDV DVLAPAAIEE 310 320 330 340 350 360 VITKKNADNI KAKIVAEVAN GPVTPEADEI LFEKGILQIP DFLCNAGGVT VSYFEWVQNI 370 380 390 400 410 420 TGYYWTLEEV RERLDKKMTK AFYDVYNTAK EKNIHMRDAA YVVAVQRVYQ AMLDRGWVKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Gonzalez J.M., Robb F.T., Kato C. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JA1.
[2]	"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OT3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF035935 Genomic DNA. Translation: AAB99956.1. BA000001 Genomic DNA. Translation: BAA30705.1. Different initiation.
PIR	A71038.
RefSeq	NP_143449.1.
3D structure databases
HSSP	HSSP built from PDB template 1GTM based on UniProtKB P80319.
SMR	O52310. Positions 5-420.
ModBase	Search...
Genome annotation databases
GeneID	1442446.
GenomeReviews	Gene locus PH1593 in contig BA000001_GR.
KEGG	pho:PH1593.
NMPDR	fig|70601.1.peg.1560.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O52310.
OMA	O52310. YLAKIMS.
Enzyme and pathway databases
BRENDA	1.4.1.3. 74679.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE3_PYRHO

Accession

Primary (citable) accession number: O52310

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)