DNA-directed RNA polymerase subunit beta - Rickettsia prowazekii (strain Madrid E)

Preferred order of sections

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	RP140

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	1374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Antibiotic resistance Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1374

1374

DNA-directed RNA polymerase subunit beta HAMAP MF_01321

PRO_0000047950

Natural variations

Natural variant

10

1

S → A in strain: Breinl.

Natural variant

15

1

H → L in strain: Breinl.

Natural variant

19

1

I → L in strain: Breinl.

Natural variant

96

1

D → E in strain: Breinl.

Natural variant

195

1

R → I in strain: Breinl.

Natural variant

216

1

K → R in strain: Breinl.

Natural variant

256

1

K → E in strain: Breinl.

Natural variant

273

1

D → Y in strain: Breinl.

Natural variant

282

1

K → N in strain: Breinl.

Natural variant

299

1

G → S in strain: Breinl.

Natural variant

310

1

L → F in strain: Breinl.

Natural variant

546

1

R → K in rifampicin resistant mutant.

Sequences

Sequence

Length

Mass (Da)

Tools

O52271 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 384676DFF6584DB4
Show »

FASTA

1,374

154,583

        10         20         30         40         50         60 
MVSLRDNIES QPLSHNRRIR KNFGHINLVA DIPNLIEIQK NSYEKNFLQL NIKDSERKNK 

        70         80         90        100        110        120 
GLQSILNSIF PISDSSNIAN LEFVKYEFDT PKYDVDECSQ RSLSYAAPLK VTLRLSIWDI 

       130        140        150        160        170        180 
DEDTGTREIK GIKEQEVYMG DIPLMTKNGT FIINGTERVV VSQMHRSPGV FFYHDEGKVH 

       190        200        210        220        230        240 
SSGKLLYSAR VIPYRGSWLD FEFDAKDIIY FRIDRKRKLY ATTLLRAIGM STEEIIKFYY 

       250        260        270        280        290        300 
NSVTYKFVKN KGWSVKFIPQ HITAHRLTSD LVDADTGNVL LKAGQKITPR LAQKYFGVGL 

       310        320        330        340        350        360 
NNILVTHETL IGKYLSEDLR DPASDEVLAK IGEMITSDML KVINDLKIKN VNVLVINPQS 

       370        380        390        400        410        420 
GSYIRNTLFA DKNQDREAAL CDIFRVLRPG EPANIEAAES LFYNLFFDAE RYDLSEVGRI 

       430        440        450        460        470        480 
KMNSRLELNI SEEVTVLTID DIKNIVRILV ELKDGKGIID DIDHLGNRRV RSVGELIENQ 

       490        500        510        520        530        540 
FRIGLVRMEK SVIERMSAGD VDTVMPHDLV NSKILVSVVK EFFSTSQLSQ FMDQTNPLSE 

       550        560        570        580        590        600 
ITHKRRLSAL GPGGLSRDRA GFEVRDVHPT HYGRICPIET PEGQNIGLIN SMATYARINK 

       610        620        630        640        650        660 
HGFIESPYRR VKNGYVTDEV VYLSAIEEGK YKIGQANSKV DQDGKLQGEF INCRVEGGNF 

       670        680        690        700        710        720 
VMVEPDEVDF IDVTPMQVVS VAASLIPFLE NDDANRALMG SNMQRQAVPL IKTEAPFVGT 

       730        740        750        760        770        780 
GVEGVVAKDS GASVLALHDG IVERVDSNRI VIRTLEQKVD GSPSVDIYNL LKFQKSNHNT 

       790        800        810        820        830        840 
CINQKPLVKV GHYVKKNDII ADGPSTDNGE IALGRNVLVA FLPWNGYNFE DSILISERIV 

       850        860        870        880        890        900 
KEDVFTSIHI EEFEVIARDT RLGPEEITRD IPNVSEEALR HLDEVGIIYV GAEVKAGDIL 

       910        920        930        940        950        960 
VGKVTPKSES PITPEEKLLR AIFGEKAFDV KDSSLHVPSG VSGTVVEVRI FSRRGVEKDQ 

       970        980        990       1000       1010       1020 
RAIAIEKQQI EKLAKDRDDE LEIIEHFVFS WLEKLLVGHV IINGPKQITA GQTITTEMLK 

      1030       1040       1050       1060       1070       1080 
GLSKGQLWQI TVEDANVMNE IEQIKIHYDE KKYALDKRFT TKVEKLQSGD DLPQGALKVV 

      1090       1100       1110       1120       1130       1140 
KVFIATKHKL QPGDKMAGRH GNKGVISRIV PEEDMPFLED GTVVDIVLNP LGLPSRMNIG 

      1150       1160       1170       1180       1190       1200 
QILETHLGWA SINLAKKIST LVKEYKDNHI DIEEIKKFLL ELYGKDINYI LEGSEEEIIS 

      1210       1220       1230       1240       1250       1260 
FCNKVSKGVY FATPVFDGAK VQDVKDMLEL AGQDLSGQVK LIDGRTGEYF DRLVTVGHKY 

      1270       1280       1290       1300       1310       1320 
LLKLHHLVDN KIHSRSIGPY SLVTQQPLGG KSHFGGQRFG EMECWALQAY GAAYTLQEML 

      1330       1340       1350       1360       1370 
TVKSDDVNGR IKTYDSIVRG ENNFESGIPE SFNVMIKEFR SLCLNVKLEV TPSK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Transformation of Rickettsia prowazekii to rifampin resistance." Rachek L.I., Tucker A.M., Winkler H.H., Wood D.O. J. Bacteriol. 180:2118-2124(1998) [PubMed: 9555894] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Madrid E.
[2]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
[3]	"Characterization of mutations in the rpoB gene in naturally rifampin-resistant Rickettsia species." Drancourt M., Raoult D. Antimicrob. Agents Chemother. 43:2400-2403(1999) [PubMed: 10508014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC VR-142 / Breinl.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF034531 Genomic DNA. Translation: AAC38354.1. AJ235270 Genomic DNA. Translation: CAA14608.1. AF076437 Genomic DNA. Translation: AAF22439.1.
PIR	A71724.
RefSeq	NP_220531.1. NC_000963.1.
3D structure databases
ProteinModelPortal	O52271.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883777.
GenomeReviews	Gene locus RP140 in contig AJ235269_GR.
KEGG	rpr:RP140.
PATRIC	17901022. VBIRicPro72556_0142.
Phylogenomic databases
HOGENOM	HBG285547.
OMA	QRDLTYA.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	RPRO272947:RP140-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_RICPR

Accession

Primary (citable) accession number: O52271
Secondary accession number(s): Q9RH37

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Rickettsia prowazekii

Rickettsia prowazekii (strain Madrid E): entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents