ID   CHEB1_PSEPU             Reviewed;         374 AA.
AC   O52262; Q9L942;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   16-JUN-2009, entry version 65.
DE   RecName: Full=Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon;
DE            EC=3.1.1.61;
GN   Name=cheB;
OS   Pseudomonas putida.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PRS2000;
RX   MEDLINE=98164369; PubMed=9503621;
RX   DOI=10.1111/j.1574-6968.1998.tb12871.x;
RA   Ditty J.L., Grimm A.C., Harwood C.S.;
RT   "Identification of a chemotaxis gene region from Pseudomonas putida.";
RL   FEMS Microbiol. Lett. 159:267-273(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MK1;
RX   MEDLINE=20253319; PubMed=10792727;
RX   DOI=10.1046/j.1365-2958.2000.01859.x;
RA   Pandza S., Baetens M., Park C.H., Au T., Keyhan M., Matin A.;
RT   "The G-protein FlhF has a role in polar flagellar placement and
RT   general stress response induction in Pseudomonas putida.";
RL   Mol. Microbiol. 36:414-423(2000).
CC   -!- FUNCTION: Involved in the modulation of the chemotaxis system;
CC       catalyzes the demethylation of specific methylglutamate residues
CC       introduced into the chemoreceptors (methyl-accepting chemotaxis
CC       proteins) by cheR (By similarity).
CC   -!- CATALYTIC ACTIVITY: Protein L-glutamate O(5)-methyl ester + H(2)O
CC       = protein L-glutamate + methanol.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- DOMAIN: The N-terminal regulatory domain inhibits the activity of
CC       the C-terminal effector domain.
CC   -!- PTM: Phosphorylated by cheA. Phosphorylation suppresses the
CC       inhibitory activity of the N-terminal domain (By similarity).
CC   -!- SIMILARITY: Contains 1 cheB-type methylesterase domain.
CC   -!- SIMILARITY: Contains 1 response regulatory domain.
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DR   EMBL; AF031898; AAC08065.1; -; Genomic_DNA.
DR   EMBL; AF183382; AAF67048.1; -; Genomic_DNA.
DR   HSSP; P04042; 1CHD.
DR   BRENDA; 3.1.1.61; 403.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008984; F:protein-glutamate methylesterase activity; IEA:HAMAP.
DR   GO; GO:0000156; F:two-component response regulator activity; IEA:InterPro.
DR   GO; GO:0006935; P:chemotaxis; IEA:HAMAP.
DR   GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro.
DR   GO; GO:0007606; P:sensory perception of chemical stimulus; IEA:HAMAP.
DR   GO; GO:0000160; P:two-component signal transduction system (p...; IEA:HAMAP.
DR   HAMAP; MF_00099; -; 1.
DR   InterPro; IPR008248; Sig_transdc_resp-reg_CheB.
DR   InterPro; IPR000673; Sig_transdc_resp-reg_Me-estase.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   Gene3D; G3DSA:3.40.50.180; Chemotax_RR_pGlu_Me-esterase; 1.
DR   Pfam; PF01339; CheB_methylest; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   PIRSF; PIRSF000876; RR_chemtxs_CheB; 1.
DR   ProDom; PD005328; CheB_methylest; 1.
DR   ProDom; PD000039; Response_reg; 1.
DR   SMART; SM00448; REC; 1.
DR   PROSITE; PS50122; CHEB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Chemotaxis; Cytoplasm; Hydrolase; Phosphoprotein.
FT   CHAIN         1    374       Chemotaxis response regulator protein-
FT                                glutamate methylesterase of group 1
FT                                operon.
FT                                /FTId=PRO_0000158013.
FT   DOMAIN        4    121       Response regulatory.
FT   DOMAIN      183    374       CheB-type methylesterase.
FT   ACT_SITE    198    198       By similarity.
FT   ACT_SITE    225    225       By similarity.
FT   ACT_SITE    318    318       By similarity.
FT   MOD_RES      55     55       4-aspartylphosphate (By similarity).
FT   VARIANT     141    147       RPAPVAA -> LAGAGRR (in MK1).
FT   VARIANT     193    194       VA -> LR (in MK1).
SQ   SEQUENCE   374 AA;  39696 MW;  47ED6595DF7BE388 CRC64;
     MAVKVLVVDD SGFFRRRVSE ILSADPTIQV VGTATNGKEA IDQALALKPD VITMDYEMPM
     MDGITAVRHI MQRCPTPVLM FSSLTHEGAR VTLDALDAGA VDYLPKNFED ISRNPDKVKQ
     MLCEKVHTIS RSNRRLGSYA RPAPVAAPVP ASSPAPASSF ASPAPARPAA TARAAAPAAS
     HSPAPKRKPY KLVAIGTSTG GPVALQRVLT QLPANFPAPI VLIQHMPAAF TKAFAERLDK
     LCRINVKEAE DGDMLRPGLA LLAPGGKQMM IDGRGTVKIL PGDERLNYKP CVDITFGSAA
     KSYGDKVLSV VLTGMGADGR EGARLLKQGG STVWAQDEAS CVIYGMPMAI VKANLADAVY
     SLDDIGKHLV EACV
//