O52262 (CHEB1_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon
EC=3.1.1.61

Gene names

Name:

cheB

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon HAMAP MF_00099		PRO_0000158013
Regions
	Domain	4 – 121	118	Response regulatory
	Domain	183 – 374	192	CheB-type methylesterase
Sites
	Active site	198	1	By similarity
	Active site	225	1	By similarity
	Active site	318	1	By similarity
Amino acid modifications
	Modified residue	55	1	4-aspartylphosphate By similarity
Natural variations
	Natural variant	141 – 147	7	RPAPVAA → LAGAGRR in MK1.
	Natural variant	193 – 194	2	VA → LR in MK1.

Sequences

	Sequence		Length	Mass (Da)
	O52262 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 47ED6595DF7BE388	FASTA	374	39,696
	10 20 30 40 50 60 MAVKVLVVDD SGFFRRRVSE ILSADPTIQV VGTATNGKEA IDQALALKPD VITMDYEMPM 70 80 90 100 110 120 MDGITAVRHI MQRCPTPVLM FSSLTHEGAR VTLDALDAGA VDYLPKNFED ISRNPDKVKQ 130 140 150 160 170 180 MLCEKVHTIS RSNRRLGSYA RPAPVAAPVP ASSPAPASSF ASPAPARPAA TARAAAPAAS 190 200 210 220 230 240 HSPAPKRKPY KLVAIGTSTG GPVALQRVLT QLPANFPAPI VLIQHMPAAF TKAFAERLDK 250 260 270 280 290 300 LCRINVKEAE DGDMLRPGLA LLAPGGKQMM IDGRGTVKIL PGDERLNYKP CVDITFGSAA 310 320 330 340 350 360 KSYGDKVLSV VLTGMGADGR EGARLLKQGG STVWAQDEAS CVIYGMPMAI VKANLADAVY 370 SLDDIGKHLV EACV

References

[1]	"Identification of a chemotaxis gene region from Pseudomonas putida." Ditty J.L., Grimm A.C., Harwood C.S. FEMS Microbiol. Lett. 159:267-273(1998) [PubMed: 9503621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PRS2000.
[2]	"The G-protein FlhF has a role in polar flagellar placement and general stress response induction in Pseudomonas putida." Pandza S., Baetens M., Park C.H., Au T., Keyhan M., Matin A. Mol. Microbiol. 36:414-423(2000) [PubMed: 10792727] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MK1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF031898 Genomic DNA. Translation: AAC08065.1. AF183382 Genomic DNA. Translation: AAF67048.1.
3D structure databases
ProteinModelPortal	O52262.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_PSEPU

Accession

Primary (citable) accession number: O52262
Secondary accession number(s): Q9L942

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	September 21, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program