Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Toluene efflux pump membrane transporter TtgB

Gene

ttgB

Organism
Pseudomonas putida (strain DOT-T1E)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

The inner membrane transporter component of a constitutive organic solvent efflux system. Involved in export of toluene, styrene, m-xylene, propylbenzene and ethylbenzene. Also exports AMP and the antibiotics carbenicillin, nalidixic acid, chloramphenicol and tetracycline.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Transport

Protein family/group databases

TCDBi2.A.6.2.9. the resistance-nodulation-cell division (rnd) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Toluene efflux pump membrane transporter TtgB
Gene namesi
Name:ttgB
Ordered Locus Names:T1E_0242
OrganismiPseudomonas putida (strain DOT-T1E)
Taxonomic identifieri1196325 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000006503 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3021HelicalSequence analysisAdd
BLAST
Transmembranei339 – 35921HelicalSequence analysisAdd
BLAST
Transmembranei370 – 39021HelicalSequence analysisAdd
BLAST
Transmembranei393 – 41321HelicalSequence analysisAdd
BLAST
Transmembranei440 – 46021HelicalSequence analysisAdd
BLAST
Transmembranei472 – 49221HelicalSequence analysisAdd
BLAST
Transmembranei539 – 55921HelicalSequence analysisAdd
BLAST
Transmembranei871 – 89121HelicalSequence analysisAdd
BLAST
Transmembranei893 – 91321HelicalSequence analysisAdd
BLAST
Transmembranei923 – 94321HelicalSequence analysisAdd
BLAST
Transmembranei972 – 99221HelicalSequence analysisAdd
BLAST
Transmembranei1004 – 102421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10501050Toluene efflux pump membrane transporter TtgBPRO_0000161849Add
BLAST

Expressioni

Inductioni

The ttgABC operon is repressed by toluene; this is mediated by TtgR. The ttgABC operon is induced in response to chloramphenicol and tetracycline.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO52248.
SMRiO52248. Positions 4-1016.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK18138.

Family and domain databases

Gene3Di3.30.2090.10. 2 hits.
InterProiIPR027463. AcrB_DN_DC_subdom.
IPR001036. Acrflvin-R.
IPR004764. HAE1.
[Graphical view]
PfamiPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSiPR00702. ACRIFLAVINRP.
SUPFAMiSSF82714. SSF82714. 2 hits.
TIGRFAMsiTIGR00915. 2A0602. 1 hit.

Sequencei

Sequence statusi: Complete.

O52248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKFFIDRPI FAWVIALVIM LVGALSILKL PINQYPSIAP PAIAIAVTYP
60 70 80 90 100
GASAQTVQDT VVQVIEQQLN GIDNLRYVSS ESNSDGSMTI TATFEQGTNP
110 120 130 140 150
DTAQVQVQNK LNLATPLLPQ EVQQQGIRVT KAVKNFLLVI GLVSEDGSMT
160 170 180 190 200
KDDLANYIVS NMQDPISRTA GVGDFQVFGA QYAMRIWLDP AKLNKFQLTP
210 220 230 240 250
VDVKTAVAAQ NVQVSSGQLG GLPALPGTQL NATIIGKTRL QTAEQFESIL
260 270 280 290 300
LKVNKDGSQV RLGDVAQVGL GGENYAVSAQ FNGKPASGLA VKLATGANAL
310 320 330 340 350
DTAKALRETI KGLEPFFPPG VKAVFPYDTT PVVTESISGV IHTLIEAVVL
360 370 380 390 400
VFLVMYLFLQ NFRATIITTM TVPVVLLGTF GILAAAGFSI NTLTMFAMVL
410 420 430 440 450
AIGLLVDDAI VVVENVERVM SEEGLPPKEA TKRSMEQIQG ALVGIALVLS
460 470 480 490 500
AVLLPMAFFG GSTGVIYRQF SITIVSAMGL SVLVALIFTP ALCATMLKPL
510 520 530 540 550
KKGEHHTAKG GFFGWFNRNF DRSVNGYERS VGTILRNKVP FLLAYALIVV
560 570 580 590 600
GMIWLFARIP TAFLPEEDQG VLFAQVQTPA GSSAERTQVV VDQMREYLLK
610 620 630 640 650
DEADTVSSVF TVNGFNFAGR GQSSGMAFIM LKPWDERSKE NSVFALAQRA
660 670 680 690 700
QQHFFTFRDA MVFAFAPPAV LELGNATGFD VFLQDRGGVG HAKLMEARNQ
710 720 730 740 750
FLAKAAQSKI LSAVRPNGLN DEPQYQLTID DERASALGVT IADINNTLSI
760 770 780 790 800
ALGASYVNDF IDRGRVKKVY IQGEPSARMS PEDLQKWYVR NGAGEMVPFS
810 820 830 840 850
SFAKGEWTYG SPKLSRYNGV EAMEILGAPA PGYSTGEAMA EVERIAGELP
860 870 880 890 900
SGIGFSWTGM SYEEKLSGSQ MPALFALSVL FVFLCLAALY ESWSIPIAVV
910 920 930 940 950
LVVPLGIIGA LIATSLRGLS NDVYFLVGLL TTIGLAAKNA ILIVEFAKEL
960 970 980 990 1000
HEQGRSLYDA AIEACRMRLR PIIMTSLAFI LGVVPLTIAS GAGAGSQHAI
1010 1020 1030 1040 1050
GTGVIGGMIS ATVLAIFWVP LFFVAVSSLF GSKEPEKDVT PENPRYEAGQ
Length:1,050
Mass (Da):112,808
Last modified:November 1, 1998 - v2
Checksum:iCEC4552686C5E49C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031417 Genomic DNA. Translation: AAC38671.1.
CP003734 Genomic DNA. Translation: AFO46101.1.

Genome annotation databases

EnsemblBacteriaiAFO46101; AFO46101; T1E_0242.
KEGGippx:T1E_0242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031417 Genomic DNA. Translation: AAC38671.1.
CP003734 Genomic DNA. Translation: AFO46101.1.

3D structure databases

ProteinModelPortaliO52248.
SMRiO52248. Positions 4-1016.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi2.A.6.2.9. the resistance-nodulation-cell division (rnd) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFO46101; AFO46101; T1E_0242.
KEGGippx:T1E_0242.

Phylogenomic databases

KOiK18138.

Family and domain databases

Gene3Di3.30.2090.10. 2 hits.
InterProiIPR027463. AcrB_DN_DC_subdom.
IPR001036. Acrflvin-R.
IPR004764. HAE1.
[Graphical view]
PfamiPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSiPR00702. ACRIFLAVINRP.
SUPFAMiSSF82714. SSF82714. 2 hits.
TIGRFAMsiTIGR00915. 2A0602. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Efflux pumps involved in toluene tolerance in Pseudomonas putida DOT-T1E."
    Ramos J.L., Duque E., Godoy P., Segura A.
    J. Bacteriol. 180:3323-3329(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: DOT-T1E.
  2. "Metabolic potential of the organic-solvent tolerant Pseudomonas putida DOT-T1E deduced from its annotated genome."
    Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A., Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.
    Microb. Biotechnol. 6:598-611(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DOT-T1E.
  3. "Three efflux pumps are required to provide efficient tolerance to toluene in Pseudomonas putida DOT-T1E."
    Rojas A., Duque E., Mosqueda G., Golden G., Hurtado A., Ramos J.L., Segura A.
    J. Bacteriol. 183:3967-3973(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: EFFLUX PUMP SUBSTRATES.
    Strain: DOT-T1E.
  4. "Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR."
    Teran W., Felipe A., Segura A., Rojas A., Ramos J.L., Gallegos M.T.
    Antimicrob. Agents Chemother. 47:3067-3072(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: DOT-T1E.

Entry informationi

Entry nameiTTGB_PSEPT
AccessioniPrimary (citable) accession number: O52248
Secondary accession number(s): I7BZ53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: November 1, 1998
Last modified: November 11, 2015
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

There are 4 nearly identical operons in various strains of P.putida. This one and the mepABC operon of strain KT2442-TOL function in solvent and antibiotic efflux; however the arpABC operon of strain S12 functions only in antibiotic efflux. This may be due to different protein expression levels. In strain KT2440 the equivalent operon does not seem to function in toluene efflux.Curated

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.