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Protein

GTP pyrophosphokinase

Gene

relA

Organism
Myxococcus xanthus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp (By similarity).By similarity

Catalytic activityi

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Pathway: ppGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GTP.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. GTP pyrophosphokinase (relA)
  2. no protein annotated in this organism
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GTP, the pathway ppGpp biosynthesis and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00908; UER00884.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP pyrophosphokinase (EC:2.7.6.5)
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I
Gene namesi
Name:relA
OrganismiMyxococcus xanthus
Taxonomic identifieri34 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeMyxococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757GTP pyrophosphokinasePRO_0000166553Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246197.MXAN_3204.

Structurei

3D structure databases

ProteinModelPortaliO52177.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 165103HDAdd
BLAST
Domaini685 – 75773ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated
Contains 1 ACT domain.PROSITE-ProRule annotation
Contains 1 HD domain.Curated

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O52177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGNVAPPLV QSYDCSSFDD SPERHPPTVS QYHPDPDLDI IKKAYVYSAK
60 70 80 90 100
VHQGQLRKSG EPYLVHPLEV AGILGELKLD EASIVTGLLH DTIEDTLATE
110 120 130 140 150
EELTELFGSE VAHLVDGVTK LSKFSASASL SQEEKQAENF RKMIIAMAQD
160 170 180 190 200
IRVILVKLAD RTHNMRTLDH MSEEKQARIA QETLDIYAPL ANRLGISWIK
210 220 230 240 250
TELEDLSFRY VKPQEFFALQ AKLNKRKKER EKYIEDTCDL IRSKLAERGL
260 270 280 290 300
KGEVSGRFKH VYSIYKKIKS QGIDFDQIHD IIAFRIIAPT APSCYEALGL
310 320 330 340 350
VHEMWKPVPG RFKDFIAIPK PNMYQSLHTT IIGPLSERVE VQIRTSEMHK
360 370 380 390 400
IAEEGIAAHW KYKEGKAVIS KDDEKFAWLR QLMEWQQDLK DPKEFLETVK
410 420 430 440 450
VDLFTDEVFV FTPKGDVRSL PRGATPVDFA YAIHSDVGNR CVGAKVNGKI
460 470 480 490 500
VPLRYKMKNG DTVEVLTSPQ QHPSKDWLTF VKTSRAQQRI RGFIKQQQRE
510 520 530 540 550
KSLQLGRELA DRELKRFQLN FNRLLKSGEM KKAAVDLGFR VEDDMLVAIG
560 570 580 590 600
YGKVTPQQLS HRLVPQEKLN AAEAGGRADA NPAATTSGGA GNSVLPGLSR
610 620 630 640 650
VTDLAKRLVG RSNRSGVQIG GVDDVLVRFG RCCNPVPGDP IAGFITRGRG
660 670 680 690 700
VTVHTVGCEK ALATDPERRV DVSWDVRGDF KRPVTLRVLT ADRPGLLADI
710 720 730 740 750
TNTFSKKGVN ISQANCRATG DDRAVNTFEV IISDLKQLTD LMRTIERLQG

VYSVERI
Length:757
Mass (Da):84,978
Last modified:June 1, 1998 - v1
Checksum:iD6CC1000A5F72A7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025847 Genomic DNA. Translation: AAB97677.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025847 Genomic DNA. Translation: AAB97677.1.

3D structure databases

ProteinModelPortaliO52177.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246197.MXAN_3204.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00908; UER00884.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The guanosine nucleotide (p)ppGpp initiates development and A-factor production in Myxococcus xanthus."
    Harris B.Z., Kaiser D., Singer M.H.
    Genes Dev. 12:1022-1035(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DK101.

Entry informationi

Entry nameiRELA_MYXXA
AccessioniPrimary (citable) accession number: O52177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: June 24, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.