O52058 (ACCC_ALLVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin carboxylase
EC=6.3.4.14

Alternative name(s):
Acetyl-CoA carboxylase subunit A
Short name=ACC
EC=6.4.1.2

Gene names

Name:	accC
Ordered Locus Names:	Alvin_1906

Organism

Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / D) (Chromatium vinosum) [Complete proteome] [HAMAP]

Taxonomic identifier

572477 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Chromatiaceae › Allochromatium

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA By similarity.
Catalytic activity	ATP + biotin-[carboxyl-carrier-protein] + CO2 = ADP + phosphate + carboxy-biotin-[carboxyl-carrier-protein]. ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA.
Pathway	Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
Subunit structure	Acetyl-CoA carboxylase is an heterohexamer of biotin carboxyl carrier protein, biotin carboxylase and the two subunits of carboxyl transferase in a 2:2 complex By similarity.
Sequence similarities	Contains 1 ATP-grasp domain. Contains 1 biotin carboxylation domain.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Ligand	ATP-binding Biotin Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetyl-CoA carboxylase activity Inferred from electronic annotation. Source: EC biotin carboxylase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Biotin carboxylase		PRO_0000146790
Regions
	Domain	4 – 448	445	Biotin carboxylation
	Domain	123 – 320	198	ATP-grasp
Sites
	Active site	295	1	Potential
Experimental info
	Sequence conflict	427	1	I → L in AAB91545. Ref.2
	Sequence conflict	437	1	Missing in AAB91545. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O52058 [UniParc]. Last modified June 15, 2010. Version 2. Checksum: 40510894E20C82E4	FASTA	449	49,268
	10 20 30 40 50 60 MSAMIEKVLI ANRGEIALRI LRACRELGIK TVAVHSEADR DLKHVLLADE SVCIGPAPAM 70 80 90 100 110 120 QSYLNVPAII SAAEVTDTVA IHPGYGFLSE NADFAERVEK SGFIFIGPRP ETIRLMGDKV 130 140 150 160 170 180 SAIAAMKAAG VPCVPGSDGP IDDNKKRTLE LAREIGYPIM IKSSGGGGGR GMRVVHSEAT 190 200 210 220 230 240 LLNAIALTRA EAAAAFNNDM VYMEKYLENP RHIEFQVLAD QMGNAIHLGE RDCSMQRRHQ 250 260 270 280 290 300 KVVEEAPAPG ITEEQRREIG ERCAAACRSI GYRGAGTFEF LYENGQFYFI EMNTRVQVEH 310 320 330 340 350 360 PVTEMVTGVD IVKEQILIAA GEPLRYRQSD IQMRGHAIEC RINAEHPETF MPSPGKITDF 370 380 390 400 410 420 HAPGGPGVRI ETHIYSGYTV PCHYDSMIGK LITHGEDRES AVARMCNALR ETVIEGIHSN 430 440 IKLQRSIMRD GAFLAGGANI HYLEKMLGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of chromosome of Allochromatium vinosum DSM 180." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Munk A.C., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Zigann R., Dahl C., Woyke T. Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17899 / DSM 180 / NBRC 103801 / D.
[2]	"Molecular genetic evidence for extracytoplasmic localization of sulfur globules in Chromatium vinosum." Pattaragulwanit K., Brune D.C., Trueper H.G., Dahl C. Arch. Microbiol. 169:434-444(1998) [PubMed: 9560425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 309-449.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001896 Genomic DNA. Translation: ADC62830.1. AF017119 Genomic DNA. Translation: AAB91545.1.
RefSeq	YP_003443862.1. NC_013851.1.
3D structure databases
ProteinModelPortal	O52058.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8787270.
GenomeReviews	Gene locus Alvin_1906 in contig CP001896_GR.
KEGG	alv:Alvin_1906.
PATRIC	31923588. VBIAllVin64954_1890.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR004549. Acetyl_CoA_COase_biotin_COase. IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR011764. Biotin_carboxylation_dom. IPR005482. Biotin_COase_C. IPR005479. CarbamoylP_synth_lsu_ATP-bd. IPR005481. CarbamoylP_synth_lsu_N. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01961.
Pfam	PF02785. Biotin_carb_C. 1 hit. PF00289. CPSase_L_chain. 1 hit. PF02786. CPSase_L_D2. 1 hit. [Graphical view]
SMART	SM00878. Biotin_carb_C. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00514. AccC. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS50979. BC. 1 hit. PS00866. CPSASE_1. 1 hit. PS00867. CPSASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACCC_ALLVD

Accession

Primary (citable) accession number: O52058
Secondary accession number(s): D3RUH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 15, 2010
Last modified:	January 25, 2012
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program