ID   GVPO1_HALSA             Reviewed;         119 AA.
AC   O51968; Q48313;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   27-MAR-2024, entry version 104.
DE   RecName: Full=Gas vesicle protein O1 {ECO:0000303|PubMed:21158390};
DE            Short=GvpO1 {ECO:0000303|PubMed:21158390};
DE   AltName: Full=p-GvpO {ECO:0000303|PubMed:7651141};
GN   Name=gvpO {ECO:0000303|PubMed:1404376};
GN   Synonyms=p-gvpO {ECO:0000303|PubMed:7651141};
GN   OrderedLocusNames=VNG_5034G;
GN   and
GN   Name=gvpO1 {ECO:0000312|EMBL:AAG20729.1};
GN   OrderedLocusNames=VNG_6033G {ECO:0000312|EMBL:AAG20729.1};
OS   Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS   (Halobacterium halobium).
OG   Plasmid pNRC100, Plasmid pNRC200, and Plasmid pHH1.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium; Halobacterium salinarum NRC-34001.
OX   NCBI_TaxID=64091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND GAS VESICLE GENE CLUSTER.
RC   STRAIN=NRC-817; PLASMID=pHH1;
RX   PubMed=1404376; DOI=10.1016/0022-2836(92)90914-6;
RA   Englert C., Krueger K., Offner S., Pfeifer F.;
RT   "Three different but related gene clusters encoding gas vesicles in
RT   halophilic archaea.";
RL   J. Mol. Biol. 227:586-592(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1; PLASMID=pNRC100;
RX   PubMed=9847077; DOI=10.1101/gr.8.11.1131;
RA   Ng W.V., Ciufo S.A., Smith T.M., Bumgarner R.E., Baskin D., Faust J.,
RA   Hall B., Loretz C., Seto J., Slagel J., Hood L., DasSarma S.;
RT   "Snapshot of a large dynamic replicon in a halophilic archaeon: megaplasmid
RT   or minichromosome?";
RL   Genome Res. 8:1131-1141(1998).
RN   [3] {ECO:0000312|EMBL:AAG20729.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1; PLASMID=pNRC200;
RX   PubMed=11016950; DOI=10.1073/pnas.190337797;
RA   Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA   Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA   Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA   Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA   Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA   Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA   Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA   DasSarma S.;
RT   "Genome sequence of Halobacterium species NRC-1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
RN   [4]
RP   FUNCTION, AND GAS VESICLE PRODUCTION.
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1; PLASMID=pNRC100;
RX   PubMed=1398080; DOI=10.1016/0378-1119(92)90078-4;
RA   Halladay J.T., Ng W.L., DasSarma S.;
RT   "Genetic transformation of a halophilic archaebacterium with a gas vesicle
RT   gene cluster restores its ability to float.";
RL   Gene 119:131-136(1992).
RN   [5]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1; PLASMID=pNRC100;
RX   PubMed=8002589; DOI=10.1128/jb.176.24.7646-7652.1994;
RA   DasSarma S., Arora P., Lin F., Molinari E., Yin L.R.;
RT   "Wild-type gas vesicle formation requires at least ten genes in the gvp
RT   gene cluster of Halobacterium halobium plasmid pNRC100.";
RL   J. Bacteriol. 176:7646-7652(1994).
RN   [6]
RP   FUNCTION, AND INDUCTION.
RC   STRAIN=PHH1;
RX   PubMed=7651141; DOI=10.1111/j.1365-2958.1995.tb02387.x;
RA   Offner S., Pfeifer F.;
RT   "Complementation studies with the gas vesicle-encoding p-vac region of
RT   Halobacterium salinarium PHH1 reveal a regulatory role for the p-gvpDE
RT   genes.";
RL   Mol. Microbiol. 16:9-19(1995).
RN   [7]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=PHH1;
RX   PubMed=8606186; DOI=10.1128/jb.178.7.2071-2078.1996;
RA   Offner S., Wanner G., Pfeifer F.;
RT   "Functional studies of the gvpACNO operon of Halobacterium salinarium
RT   reveal that the GvpC protein shapes gas vesicles.";
RL   J. Bacteriol. 178:2071-2078(1996).
RN   [8]
RP   FUNCTION IN BUOYANCY, AND POSSIBLE INDUCTION BY OXYGEN LIMITATION.
RC   STRAIN=PHH1;
RX   PubMed=33711860; DOI=10.1099/00221287-143-2-467;
RA   Beard S.J., Hayes P.K., Walsby A.E.;
RT   "Growth competition between Halobacterium salinarium strain PHH1 and
RT   mutants affected in gas vesicle synthesis.";
RL   Microbiology 143:467-473(1997).
RN   [9]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=PHH1; PLASMID=pHH1;
RX   PubMed=10894744; DOI=10.1128/jb.182.15.4328-4336.2000;
RA   Offner S., Hofacker A., Wanner G., Pfeifer F.;
RT   "Eight of fourteen gvp genes are sufficient for formation of gas vesicles
RT   in halophilic archaea.";
RL   J. Bacteriol. 182:4328-4336(2000).
RN   [10]
RP   SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX   PubMed=21158390; DOI=10.1021/pr1009383;
RA   Chu L.J., Chen M.C., Setter J., Tsai Y.S., Yang H., Fang X., Ting Y.S.,
RA   Shaffer S.A., Taylor G.K., von Haller P.D., Goodlett D.R., Ng W.V.;
RT   "New structural proteins of Halobacterium salinarum gas vesicle revealed by
RT   comparative proteomics analysis.";
RL   J. Proteome Res. 10:1170-1178(2011).
RN   [11]
RP   FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC   STRAIN=PHH1; PLASMID=pHH1;
RX   PubMed=35966690; DOI=10.3389/fmicb.2022.971917;
RA   Jost A., Pfeifer F.;
RT   "Interaction of the gas vesicle proteins GvpA, GvpC, GvpN, and GvpO of
RT   Halobacterium salinarum.";
RL   Front. Microbiol. 13:971917-971917(2022).
CC   -!- FUNCTION: A minor component of the gas vesicle, also found in soluble
CC       extracts (PubMed:21158390). May play a role in transcription and/or RNA
CC       stability and in GV assembly (PubMed:8606186, PubMed:35966690)
CC       (Probable). Gas vesicles are hollow, gas filled proteinaceous
CC       nanostructures found in several microbial planktonic microorganisms.
CC       They allow positioning of halobacteria at the optimal depth for growth
CC       in the poorly aerated, shallow brine pools of their habitat
CC       (PubMed:33711860). {ECO:0000269|PubMed:21158390,
CC       ECO:0000269|PubMed:33711860, ECO:0000305|PubMed:35966690,
CC       ECO:0000305|PubMed:8606186}.
CC   -!- FUNCTION: Expression of a 9.5 kb p-vac DNA fragment containing 2
CC       divergently transcribed regions (gvpD-gvpE-gvpF-gvpG-gvpH-gvpI-gvpJ-
CC       gvpK-gvpL-gvpM and gvpA-gvpC-gvpN-gvpO) allows H.volcanii to produce
CC       gas vesicles (PubMed:1404376, PubMed:7651141, PubMed:10894744). A
CC       minimal gas vesicle can be made in H.volcanii by gvpA1-gvpO1 gvpF1-
CC       gvpG1-gvpJ1-gvpK1-gvpL1-gvpM1; lack of enough GvpJ1 prevents formation
CC       (PubMed:10894744). The same region restores gas vesicle production in
CC       H.halobium without the p-vac locus, but it still has the c-vac locus
CC       (PubMed:1398080). {ECO:0000269|PubMed:10894744,
CC       ECO:0000269|PubMed:1398080, ECO:0000269|PubMed:1404376,
CC       ECO:0000269|PubMed:7651141}.
CC   -!- SUBUNIT: Forms homodimers, forms a GvpN1-GvpO1 heterodimer, interacts
CC       with GvpC1 (via the latter's C-terminus), GvpF1, GvpI1 and GvpL1, might
CC       interact with GvpA1. {ECO:0000269|PubMed:35966690}.
CC   -!- SUBCELLULAR LOCATION: Gas vesicle {ECO:0000269|PubMed:21158390}.
CC       Cytoplasm {ECO:0000305|PubMed:21158390}. Note=Probably on the exterior
CC       surface of the gas vesicle. {ECO:0000305|PubMed:1404376}.
CC   -!- INDUCTION: Maximally transcribed in late log phase, probably part of a
CC       gvpC1-gvpO1 operon (Probable). Has several transcripts with and without
CC       other genes in the locus, expressed from early- to late-log but not in
CC       stationary phase (PubMed:8606186). Gas vesicles appear earlier when
CC       grown in static culture, possibly due to O(2)-limitation
CC       (PubMed:33711860). {ECO:0000269|PubMed:33711860,
CC       ECO:0000269|PubMed:8606186, ECO:0000305|PubMed:7651141}.
CC   -!- DISRUPTION PHENOTYPE: Gas vesicle formation is wild-type (strain NRC-
CC       1), expressed in situ (PubMed:8002589). No gas vesicle formation,
CC       strongly decreased accumulation of gvpACN transcripts (strain PHH1),
CC       expressed in H.volcanii (PubMed:8606186, PubMed:10894744).
CC       {ECO:0000269|PubMed:10894744, ECO:0000269|PubMed:8002589,
CC       ECO:0000269|PubMed:8606186}.
CC   -!- MISCELLANEOUS: Encoded in a 14-gene plasmid locus called p-vac which
CC       produces predominantly short, spindle-shaped gas vesicles during all
CC       stages of growth. {ECO:0000269|PubMed:1404376,
CC       ECO:0000269|PubMed:33711860}.
CC   -!- SIMILARITY: Belongs to the gas vesicle GvpO family. {ECO:0000305}.
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DR   EMBL; X64729; CAA45983.1; -; Genomic_DNA.
DR   EMBL; AF016485; AAC82812.1; -; Genomic_DNA.
DR   EMBL; AE004438; AAG20729.1; -; Genomic_DNA.
DR   PIR; T08245; T08245.
DR   RefSeq; WP_010890516.1; NZ_BK010831.1.
DR   AlphaFoldDB; O51968; -.
DR   SMR; O51968; -.
DR   GeneID; 5954628; -.
DR   KEGG; hal:gvpO; -.
DR   KEGG; hal:VNG_6033G; -.
DR   PATRIC; fig|64091.14.peg.2102; -.
DR   HOGENOM; CLU_142302_0_0_2; -.
DR   InParanoid; O51968; -.
DR   OrthoDB; 205220at2157; -.
DR   PhylomeDB; O51968; -.
DR   Proteomes; UP000000554; Plasmid pNRC100.
DR   Proteomes; UP000000554; Plasmid pNRC200.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0031411; C:gas vesicle; IEA:UniProtKB-SubCell.
DR   GO; GO:0031412; P:gas vesicle organization; IEA:InterPro.
DR   InterPro; IPR008634; Gas-vesicle_GvpO.
DR   Pfam; PF05800; GvpO; 1.
DR   PIRSF; PIRSF028743; GvpO_protein; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Gas vesicle; Plasmid; Reference proteome.
FT   CHAIN           1..119
FT                   /note="Gas vesicle protein O1"
FT                   /id="PRO_0000182702"
FT   REGION          1..48
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        24..45
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        29..30
FT                   /note="SP -> PSA (in Ref. 1; CAA45983)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        43
FT                   /note="R -> A (in Ref. 1; CAA45983)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   119 AA;  13232 MW;  D87326A45384E46F CRC64;
     MADPANDRSE REEGGEDDET PPASDGNPSP SANSFTLSNA QTRAREAAQD LLEHQFEGMI
     KAESNDEGWR TVVEVVERNA VPDTQDIIGR YEITLDGTGD VTGYELLERY RRGDMKEEL
//