O51927 (DDL_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-alanine--D-alanine ligase
EC=6.3.2.4

Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase

Gene names

Name:	ddl
Synonyms:	ddlB
Ordered Locus Names:	BUsg_208

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	D-alanine--D-alanine ligase HAMAP-Rule MF_00047		PRO_0000177798
Regions
	Domain	101 – 303	203	ATP-grasp
	Nucleotide binding	134 – 189	56	ATP By similarity
Sites
	Active site	15	1	By similarity
	Active site	150	1	By similarity
	Active site	281	1	By similarity
	Metal binding	257	1	Magnesium or manganese 1 By similarity
	Metal binding	270	1	Magnesium or manganese 1 By similarity
	Metal binding	270	1	Magnesium or manganese 2 By similarity
	Metal binding	272	1	Magnesium or manganese 2 By similarity
Experimental info
	Sequence conflict	12 – 13	2	NS → IL in AAC46067. Ref.1
	Sequence conflict	287 – 291	5	AKSIG → SKKYR in AAC46067. Ref.1
	Sequence conflict	295 – 296	2	DE → VN in AAC46067. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O51927 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: 0AA64721CCEBD515	FASTA	306	34,388
	10 20 30 40 50 60 MKKKIAVLLG GNSSERKISI KSGYAILQSL LRSGFNAYAI DTRDFPIMQL KKQGFDSAYI 70 80 90 100 110 120 ALHGTGGEDG SIQGILEYLN IPYTGSGIMS SAISLDKWRT KLLWKSLSLR VLPDIYLQKK 130 140 150 160 170 180 DISKYTYSYI LKKILKLKFP VVIKPNNAGS SIGITIVNHP DLLIDSINLA FNYSNNIIIE 190 200 210 220 230 240 KFLKGTEYTV SILNKKVLPP IKIITKNNFY DYSSKYIESS TEYICPSGLN YQKEEELKKI 250 260 270 280 290 300 VEIAWNSLGC KGCGRIDAIL DNKDKFWLLE INTIPGMTHR SLVPMAAKSI GISFDELILK ILKINK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of ftsZ, the cell division gene of Buchnera aphidicola (endosymbiont of aphids) and detection of the product." Baumann L., Baumann P. Curr. Microbiol. 36:85-89(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF012886 Genomic DNA. Translation: AAC46067.1. AE013218 Genomic DNA. Translation: AAM67772.1.
RefSeq	NP_660561.1. NC_004061.1.
3D structure databases
ProteinModelPortal	O51927.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg208.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67772; AAM67772; BUsg_208.
GeneID	1005405.
KEGG	bas:BUsg208.
PATRIC	21247203. VBIBucAph100086_0215.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1181.
KO	K01921.
OMA	SSTEYIC.
ProtClustDB	PRK01372.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-274-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 2 hits. 3.40.50.20. 1 hit.
HAMAP	MF_00047. Dala_Dala_lig.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom. [Graphical view]
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 2 hits. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_BUCAP

Accession

Primary (citable) accession number: O51927

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	August 30, 2002
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program