O51926 (MURC_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	BUsg_209

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Uncertain

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP-Rule MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00046
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MurCDEF family.
Caution	Could be the product of a pseudogene.
Sequence caution	The sequence AAC46066.2 differs from that shown. Reason: Frameshift at position 99. This is a probably a natural frameshift, as it is possible that this bacterium does not produce peptidoglycan.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	Putative UDP-N-acetylmuramate--L-alanine ligase HAMAP-Rule MF_00046		PRO_0000182069
Regions
	Nucleotide binding	124 – 130	7	ATP Potential
Experimental info
	Sequence conflict	131 – 133	3	LLA → TTS Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O51926 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 5BAE93CB56B0D9C5	FASTA	483	54,852
	10 20 30 40 50 60 MNINKIKKNN FFKEKKILKI HLIGIAGSGM SGIALILFNL GYKISGSDLL ENFMTKKLKN 70 80 90 100 110 120 MGINIYFQHS EENIKYADFI IISSAIPSDN PEIKAAKKKN IPILLRAEML QIFMQFKFGI 130 140 150 160 170 180 AVSGTHGKTT LLAMIFDILN QNKLDPTVIN GGLIKSINSH AKLGFSKYFI AEADESDGSF 190 200 210 220 230 240 LCLNPTTAII TNIEPDHIDN YNGEFKLLKK TFLDFLNKIP SYGIAILCTD NKAILNILPK 250 260 270 280 290 300 IKCKVITYGF NKSAEFRITS YKQSDFISNF TLIRKKQLNN LEIILNLPGE HNALNATAAI 310 320 330 340 350 360 AFATYQKIPD EKIYQSLKNF KGTSRRFEYV GKLFIKKKSI QNKSVMLIND YGHHPTELSE 370 380 390 400 410 420 TIKTIRKSWP KKNLIMIFQP HRYTRTRNLY FDFIKILSQV DSLLILNVYS ANESFISGAD 430 440 450 460 470 480 SFSLYSDIKK IKKNVILVTN RNLILNYLLP HLNGNDIILI QGAGDIDTII NKIFILKNKK VII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of ftsZ, the cell division gene of Buchnera aphidicola (endosymbiont of aphids) and detection of the product." Baumann L., Baumann P. Curr. Microbiol. 36:85-89(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Baumann L., Baumann P. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF012886 Genomic DNA. Translation: AAC46066.2. Frameshift. AE013218 Genomic DNA. No translation available.
3D structure databases
ProteinModelPortal	O51926.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.1190.10. 1 hit. 3.40.50.720. 1 hit. 3.90.190.20. 1 hit.
HAMAP	MF_00046. MurC.
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BUCAP

Accession

Primary (citable) accession number: O51926

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program