Reviewed, UniProtKB/Swiss-Prot O51888 (ILVC_BUCAP)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	BUsg_575

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Ketol-acid reductoisomerase		PRO_0000151287
Sites
	Active site	131	1	Potential
Experimental info
	Sequence conflict	36	1	N → I in AAC38126. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O51888-1 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: E872D203A9A219E2	FASTA	491	56,096
	10 20 30 40 50 60 MNYFNKLCFR KRINEIKKCR FMKENEFKNE NKILKNKKIV IVGCGSQGLN QALNMRDSGL 70 80 90 100 110 120 NISFALKKNS ILRKNSSWLN ATKNNFEVND YESLIPNADL VINLTPDKQH ENVVKELQKL 130 140 150 160 170 180 MKKNSCLGYS HGFNIVECGE IIRKDITVVM VAPKCPGTEV REEFKRGFGV PTLIAVHTEN 190 200 210 220 230 240 DPKKIGLEIA KAWAFSTGGH RAGVLESSFI AEVKSDLMGE QTILCGLLQT ASLICYEKLI 250 260 270 280 290 300 KDKHNPSYAA KLIQYGWETI TESLKHGGIT LMMDRLSNPS KIKAFKISKK IKKILSPLFK 310 320 330 340 350 360 KHMDDIISGS FSKEMMIDWH NNDKKLLNWR EDTKKTPFEK NILSYSEKIS EQEYYDHGTL 370 380 390 400 410 420 MVSILKSGIE LAFETMINTG IINESAYYES LHELPLIANT IARKKLYEMN IVISDTAEYG 430 440 450 460 470 480 SYLFSEAAYP ILKDFIMSLE KNVLGLSLPN TKIDNIELYK INEEIRNHPI EIVGKKLRKH 490 MKEMKSICVA K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 34.7-kb DNA segment from the genome of Buchnera aphidicola (endosymbiont of aphids) containing groEL, dnaA, the atp operon, gidA, and rho." Clark M.A., Baumann L., Baumann P. Curr. Microbiol. 36:158-163(1998) [PubMed: 9516544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF008210 Genomic DNA. Translation: AAC38126.1. AE013218 Genomic DNA. Translation: AAM68109.1.
RefSeq	NP_660898.1.
3D structure databases
HSSP	HSSP built from PDB template 1NP3 based on UniProtKB Q9HVA2.
ModBase	Search...
Genome annotation databases
GeneID	1005622.
GenomeReviews	Gene locus BUsg_575 in contig AE013218_GR.
KEGG	bas:BUsg575.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O51888.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG575-MON.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR014359. KetolA_reductoisomerase_bac. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000116. IlvC_gammaproteo. 1 hit.
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BUCAP

Accession

Primary (citable) accession number: O51888

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 30, 2002
Last modified:	November 25, 2008
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)