O51888 (ILVC_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	BUsg_575

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151287
Sites
	Active site	131	1	Potential
Experimental info
	Sequence conflict	36	1	N → I in AAC38126. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O51888 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: E872D203A9A219E2	FASTA	491	56,096
	10 20 30 40 50 60 MNYFNKLCFR KRINEIKKCR FMKENEFKNE NKILKNKKIV IVGCGSQGLN QALNMRDSGL 70 80 90 100 110 120 NISFALKKNS ILRKNSSWLN ATKNNFEVND YESLIPNADL VINLTPDKQH ENVVKELQKL 130 140 150 160 170 180 MKKNSCLGYS HGFNIVECGE IIRKDITVVM VAPKCPGTEV REEFKRGFGV PTLIAVHTEN 190 200 210 220 230 240 DPKKIGLEIA KAWAFSTGGH RAGVLESSFI AEVKSDLMGE QTILCGLLQT ASLICYEKLI 250 260 270 280 290 300 KDKHNPSYAA KLIQYGWETI TESLKHGGIT LMMDRLSNPS KIKAFKISKK IKKILSPLFK 310 320 330 340 350 360 KHMDDIISGS FSKEMMIDWH NNDKKLLNWR EDTKKTPFEK NILSYSEKIS EQEYYDHGTL 370 380 390 400 410 420 MVSILKSGIE LAFETMINTG IINESAYYES LHELPLIANT IARKKLYEMN IVISDTAEYG 430 440 450 460 470 480 SYLFSEAAYP ILKDFIMSLE KNVLGLSLPN TKIDNIELYK INEEIRNHPI EIVGKKLRKH 490 MKEMKSICVA K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 34.7-kb DNA segment from the genome of Buchnera aphidicola (endosymbiont of aphids) containing groEL, dnaA, the atp operon, gidA, and rho." Clark M.A., Baumann L., Baumann P. Curr. Microbiol. 36:158-163(1998) [PubMed: 9516544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF008210 Genomic DNA. Translation: AAC38126.1. AE013218 Genomic DNA. Translation: AAM68109.1.
RefSeq	NP_660898.1. NC_004061.1.
3D structure databases
ProteinModelPortal	O51888.
SMR	O51888. Positions 2-489.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000415; EBBUCP00000000415; EBBUCG00000000415.
GeneID	1005622.
GenomeReviews	Gene locus BUsg_575 in contig AE013218_GR.
KEGG	bas:BUsg575.
PATRIC	21248046. VBIBucAph100086_0611.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007925.
HOGENOM	HBG297205.
OMA	EKHMDDI.
ProtClustDB	PRK05225.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG575-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. IPR014359. KetolA_reductoisomerase_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit. PTHR21371:SF2. PTHR21371:SF2. 1 hit.
Pfam	PF01450. IlvC. 2 hits. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000116. IlvC_gammaproteo. 1 hit.
SUPFAM	SSF48179. 6DGDH_C_like. 2 hits.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BUCAP

Accession

Primary (citable) accession number: O51888

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 30, 2002
Last modified:	January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program