O51879 (MNMG_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Alternative name(s):
Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	BUsg_001

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	631 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP MF_00129
Cofactor	FAD By similarity. HAMAP MF_00129
Subunit structure	Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP MF_00129
Subcellular location	Cytoplasm By similarity HAMAP MF_00129.
Sequence similarities	Belongs to the MnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 631	631	tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP MF_00129		PRO_0000117074
Regions
	Nucleotide binding	15 – 20	6	FAD By similarity
	Nucleotide binding	275 – 289	15	NAD Potential
Sites
	Binding site	127	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	182	1	FAD By similarity
	Binding site	372	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O51879 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: DBBD71972F3370A3	FASTA	631	70,796
	10 20 30 40 50 60 MSKSYLKNFD VIVIGGGHAG TEAAAASARV GCKTLLLTQK ITDIGVLSCN PAIGGIGKSH 70 80 90 100 110 120 LVKEIDALGG LMARAIDYSG IQFRVLNSKK GPAVRSTRAQ ADRILYCQNI KKLLKKELNL 130 140 150 160 170 180 LILETEVKDL IVKNYQVIGV LTQSNMSFYS RSVVLSTGTF LGGKIHIGLE SYSAGRKGEK 190 200 210 220 230 240 ASIDLALRLR DLPFRVDRLK TGTPPRIDIN TINFENLFVQ HGDVPTPVFS FMGDISNHPL 250 260 270 280 290 300 QIPCFLTHTN EKTHEIIRKN LHKSPLYTGI IKGVGPRYCP SIEDKIVRFP DRKSHQIFLE 310 320 330 340 350 360 PEGLTSIEIY PNGISTSLPL DVQKEIVSSV KGLEKSKIIT PGYAVEYDFF DPKDLNLTLE 370 380 390 400 410 420 SKWIKGLFLA GQINGTTGYE EAASQGLLAG LNAALSAKDC KQWFPRRDQA YLGVLIDDLT 430 440 450 460 470 480 TQGANEPYRM FTSRSEYRLT LREDNADLRL TEIAYKLGLV DNPRWIRYNE KVLNISNEKN 490 500 510 520 530 540 RLKKIKIYPK SSDSTILNQL FNIVLTKEIN ILNLLKRPEI TYENLKYLKN FKVGISDLEA 550 560 570 580 590 600 AGQIENEIKY EGYIKRQLEE INRHLKNENT PLLPTYDYNK IKGLSHEAVL KLNDYKPVSV 610 620 630 GQASRISGIT PATISILLIH LKKEYYKNHL S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 34.7-kb DNA segment from the genome of Buchnera aphidicola (endosymbiont of aphids) containing groEL, dnaA, the atp operon, gidA, and rho." Clark M.A., Baumann L., Baumann P. Curr. Microbiol. 36:158-163(1998) [PubMed: 9516544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF008210 Genomic DNA. Translation: AAC38117.1. AE013218 Genomic DNA. Translation: AAM67573.1.
RefSeq	NP_660362.1. NC_004061.1.
3D structure databases
ProteinModelPortal	O51879.
SMR	O51879. Positions 4-555.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000430; EBBUCP00000000430; EBBUCG00000000430.
GeneID	1005814.
GenomeReviews	Gene locus BUsg_001 in contig AE013218_GR.
KEGG	bas:BUsg001.
PATRIC	21246757. VBIBucAph100086_0001.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007979.
HOGENOM	HBG284774.
OMA	GFDRSPM.
ProtClustDB	PRK05192.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG001-MONOMER.
Family and domain databases
HAMAP	MF_00129. MnmG_GidA. [Tree]
InterPro	IPR004416. GidA. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
KO	K03495.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00136. GidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNMG_BUCAP

Accession

Primary (citable) accession number: O51879

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program