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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBBUR224326:G9SO-842-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BB_0833
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliaBorrelia burgdorferi group
ProteomesiUP000001807 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10421042Isoleucine--tRNA ligasePRO_0000098525Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224326.BB_0833.

Structurei

3D structure databases

ProteinModelPortaliO51773.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

O51773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKKVENKAN FPKIEEKILK FWNDNKIFEK SIKQREGCEE FTFYDGPPFA
60 70 80 90 100
TGLPHFGHFV PNTIKDIIPR YQTMQGKYVK RNFGWDTHGL PVEYEVEKKL
110 120 130 140 150
GISGKYEIEN YGIENFNKEC RKIVLRYTEE WKNIILRLGR WVDFEKGYKT
160 170 180 190 200
MDISFMESVW WVFKNLYEKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK
210 220 230 240 250
EVNDPSLTIK FKIKDKNEYL LVWTTTPWTL PSNLGIAVGQ EIEYSKIFDK
260 270 280 290 300
TKEEILILGS KKLNSYYDDE NSYTIIEKFK GSKLEGIEYE PIFNYFLEQK
310 320 330 340 350
DKGAFKVHTA DYVTTDDGTG IVHIAPFGEE DYRILKKHTN VDIIDPLDAE
360 370 380 390 400
CKFTNQVKDF KGLFVKDADK KIIENLKLRN FLFKRENYLH RYPFCYRTNC
410 420 430 440 450
PIIYRPISSW FVNVEKIKTK LLEVNEKINW MPAHLKKGRF GKWLENAKDW
460 470 480 490 500
AISRNRFWGN PIPIWICSKT GKKICIGSKK ELENLSGQKI EDLHKDQIDK
510 520 530 540 550
ITWPSKDGGK FIRTSEVLDC WFESGAMPYA SNHYPFTNEI NFKNIFPADF
560 570 580 590 600
IAEGLDQTRG WFYTLTILGT ALFENTAFKN VIVNGLVLSS DGRKMSKSFK
610 620 630 640 650
NYTDPMQVIN TFGADALRLY LIMSPVVKAD DLKYSDNGVR DVLKNIIIPI
660 670 680 690 700
WNAYSFFTTY AIIDKFKPPK NLSLAKNNNL DKWIISELES LKKILNTEID
710 720 730 740 750
KYNLTKSIES LLEFIDKLNN WYIRRSRRRF WKSENDKDKN DAYETLYYAI
760 770 780 790 800
KTLMILLAPF IPFITEEIYQ NLKTDEDKQS IHLNDYPKAN ENFINKTIEE
810 820 830 840 850
KINLARKITS MARSLRSLHN IKIRMPISTI YIVTKNQNEQ NMLMEMQEII
860 870 880 890 900
LDEINAKEMK IKANEEELIT YKAKANFKEL GKKLGKDMKA VSTEISKLKN
910 920 930 940 950
EDIIKIINGT SYEIKVANAK HYLSLNDIIL EREEKENLKV INEESITIGI
960 970 980 990 1000
DSLITKELYL EGLTREFVRQ IQNLRKEKNF DVSDRINLYI ENNETLKEML
1010 1020 1030 1040
NKFEKYIKTE TLALNIILNK SKLEKKINLA DDIFTLIGIE KC
Length:1,042
Mass (Da):122,332
Last modified:June 1, 1998 - v1
Checksum:i6C0F7D820CA32F75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC67179.1.
PIRiH70203.
RefSeqiNP_212967.1. NC_001318.1.
WP_010889833.1. NC_001318.1.

Genome annotation databases

EnsemblBacteriaiAAC67179; AAC67179; BB_0833.
GeneIDi1195691.
KEGGibbu:BB_0833.
PATRICi20558331. VBIBorBur75917_1225.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC67179.1.
PIRiH70203.
RefSeqiNP_212967.1. NC_001318.1.
WP_010889833.1. NC_001318.1.

3D structure databases

ProteinModelPortaliO51773.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224326.BB_0833.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC67179; AAC67179; BB_0833.
GeneIDi1195691.
KEGGibbu:BB_0833.
PATRICi20558331. VBIBorBur75917_1225.

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciBBUR224326:G9SO-842-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

Entry informationi

Entry nameiSYI_BORBU
AccessioniPrimary (citable) accession number: O51773
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: June 24, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.