SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O51768

- TOP1_BORBU

UniProt

O51768 - TOP1_BORBU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
DNA topoisomerase 1
Gene
topA, BB_0828
Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi10 – 101Magnesium 1; catalytic By similarity
Sitei34 – 341Interaction with DNA By similarity
Metal bindingi97 – 971Magnesium 1; catalytic By similarity
Metal bindingi97 – 971Magnesium 2 By similarity
Metal bindingi99 – 991Magnesium 2 By similarity
Sitei152 – 1521Interaction with DNA By similarity
Sitei153 – 1531Interaction with DNA By similarity
Sitei156 – 1561Interaction with DNA By similarity
Sitei161 – 1611Interaction with DNA By similarity
Sitei168 – 1681Interaction with DNA By similarity
Active sitei324 – 3241O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei326 – 3261Interaction with DNA By similarity
Sitei511 – 5111Interaction with DNA By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBBUR224326:G9SO-837-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:BB_0828
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
ProteomesiUP000001807: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 848848DNA topoisomerase 1UniRule annotation
PRO_0000145143Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi224326.BB0828.

Structurei

3D structure databases

ProteinModelPortaliO51768.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 128125Toprim
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni176 – 1816Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Phylogenomic databases

eggNOGiCOG1754.
KOiK03168.
OMAiANEGYER.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 1 hit.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O51768-1 [UniParc]FASTAAdd to Basket

« Hide

MQNKKLIIVE SPTKAKTIKK FLDESFLVEA CIGHVVDLPN NAKEIPKEYK    50
KYEWANISID YNNGFNPIYI IPSNKKPIVS KLKKLVKTIN EIYLATDQDR 100
EGETIAFHLK EVLKIKNYKR MIFHEITETA ITESLKNTRN IDMNLVNAGE 150
ARRILDRLYG YTISPLLWKK VAYGLSAGRV QSVGLKLLIE KEKTRINFKK 200
ANYYSILLQC KHEKKNLLLE AKLEEIDGKN IAEGKDFVNE TGKLKNIAKT 250
TIITQDLMIE LEKELKNGQK IELISIETKK IKIPPPKPFT TSTLQQEINK 300
RLKIGTKQIM QHAQKLYEHG YITYMRTDSH NIAKIAKDKI TKIIKNKYGK 350
EYIEEKDRIY EKEKMAQNAH EAIRPSEIFI PNETIEIESK TAKEIYKIIW 400
DRTIISGMKD AIKENIKLTF KYKNLIFRSS FTKIIFDGFL KHTKEQDEHL 450
NINFDLIKKG DTFSIVKMKT SEHETKAPFR YTEASLVQKM EKEGIGRPST 500
YSTIISTLLE REYAFKLNNT LMPTIKGAAV INLLEKYFPV LIELNFTSNM 550
EEKLDKIAIG KLDKIKYLSK FYNGKKGLKD TVMQLEPKID SSEFRTVIES 600
QKIENKNSIN YTINIGKYGP YLIFKGHNYS INAKTPLENL YKKDEIEKII 650
NEKELKPNIL GVDPLTGLNV IFKNTIYGNI VQLGEDTHAP QEYTKKGKPK 700
KLKIIKAKKA STKKIDPENI TLELALKLLS LPKPIGKHPQ TNEQIIAATG 750
VFGDYIKTES GSIACSLKKD LKAYDITLDK AISLLNERAN KVGIIVKTIT 800
FSKNKIGNKI YIYKKNDKFY AKIKRKKIDL PDNINLEEIN EKYVFSLL 848
Length:848
Mass (Da):97,663
Last modified:June 1, 1998 - v1
Checksum:i37DEA5FB1E12FA2C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000783 Genomic DNA. Translation: AAC67161.1.
PIRiC70203.
RefSeqiNP_212962.1. NC_001318.1.

Genome annotation databases

EnsemblBacteriaiAAC67161; AAC67161; BB_0828.
GeneIDi1195686.
KEGGibbu:BB_0828.
PATRICi20558321. VBIBorBur75917_1220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000783 Genomic DNA. Translation: AAC67161.1 .
PIRi C70203.
RefSeqi NP_212962.1. NC_001318.1.

3D structure databases

ProteinModelPortali O51768.
ModBasei Search...

Protein-protein interaction databases

STRINGi 224326.BB0828.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC67161 ; AAC67161 ; BB_0828 .
GeneIDi 1195686.
KEGGi bbu:BB_0828.
PATRICi 20558321. VBIBorBur75917_1220.

Phylogenomic databases

eggNOGi COG1754.
KOi K03168.
OMAi ANEGYER.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci BBUR224326:G9SO-837-MONOMER.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 1 hit.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

Entry informationi

Entry nameiTOP1_BORBU
AccessioniPrimary (citable) accession number: O51768
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: September 3, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi