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O51768

- TOP1_BORBU

UniProt

O51768 - TOP1_BORBU

Protein

DNA topoisomerase 1

Gene

topA

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi10 – 101Magnesium 1; catalyticUniRule annotation
    Sitei34 – 341Interaction with DNAUniRule annotation
    Metal bindingi97 – 971Magnesium 1; catalyticUniRule annotation
    Metal bindingi97 – 971Magnesium 2UniRule annotation
    Metal bindingi99 – 991Magnesium 2UniRule annotation
    Sitei152 – 1521Interaction with DNAUniRule annotation
    Sitei153 – 1531Interaction with DNAUniRule annotation
    Sitei156 – 1561Interaction with DNAUniRule annotation
    Sitei161 – 1611Interaction with DNAUniRule annotation
    Sitei168 – 1681Interaction with DNAUniRule annotation
    Active sitei324 – 3241O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei326 – 3261Interaction with DNAUniRule annotation
    Sitei511 – 5111Interaction with DNAUniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBBUR224326:G9SO-837-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:BB_0828
    OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
    Taxonomic identifieri224326 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
    ProteomesiUP000001807: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 848848DNA topoisomerase 1PRO_0000145143Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224326.BB0828.

    Structurei

    3D structure databases

    ProteinModelPortaliO51768.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 128125ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni176 – 1816Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1754.
    KOiK03168.
    OMAiANEGYER.
    OrthoDBiEOG6S7XQ9.

    Family and domain databases

    Gene3Di1.10.290.10. 1 hit.
    1.10.460.10. 1 hit.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O51768-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQNKKLIIVE SPTKAKTIKK FLDESFLVEA CIGHVVDLPN NAKEIPKEYK    50
    KYEWANISID YNNGFNPIYI IPSNKKPIVS KLKKLVKTIN EIYLATDQDR 100
    EGETIAFHLK EVLKIKNYKR MIFHEITETA ITESLKNTRN IDMNLVNAGE 150
    ARRILDRLYG YTISPLLWKK VAYGLSAGRV QSVGLKLLIE KEKTRINFKK 200
    ANYYSILLQC KHEKKNLLLE AKLEEIDGKN IAEGKDFVNE TGKLKNIAKT 250
    TIITQDLMIE LEKELKNGQK IELISIETKK IKIPPPKPFT TSTLQQEINK 300
    RLKIGTKQIM QHAQKLYEHG YITYMRTDSH NIAKIAKDKI TKIIKNKYGK 350
    EYIEEKDRIY EKEKMAQNAH EAIRPSEIFI PNETIEIESK TAKEIYKIIW 400
    DRTIISGMKD AIKENIKLTF KYKNLIFRSS FTKIIFDGFL KHTKEQDEHL 450
    NINFDLIKKG DTFSIVKMKT SEHETKAPFR YTEASLVQKM EKEGIGRPST 500
    YSTIISTLLE REYAFKLNNT LMPTIKGAAV INLLEKYFPV LIELNFTSNM 550
    EEKLDKIAIG KLDKIKYLSK FYNGKKGLKD TVMQLEPKID SSEFRTVIES 600
    QKIENKNSIN YTINIGKYGP YLIFKGHNYS INAKTPLENL YKKDEIEKII 650
    NEKELKPNIL GVDPLTGLNV IFKNTIYGNI VQLGEDTHAP QEYTKKGKPK 700
    KLKIIKAKKA STKKIDPENI TLELALKLLS LPKPIGKHPQ TNEQIIAATG 750
    VFGDYIKTES GSIACSLKKD LKAYDITLDK AISLLNERAN KVGIIVKTIT 800
    FSKNKIGNKI YIYKKNDKFY AKIKRKKIDL PDNINLEEIN EKYVFSLL 848
    Length:848
    Mass (Da):97,663
    Last modified:June 1, 1998 - v1
    Checksum:i37DEA5FB1E12FA2C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000783 Genomic DNA. Translation: AAC67161.1.
    PIRiC70203.
    RefSeqiNP_212962.1. NC_001318.1.

    Genome annotation databases

    EnsemblBacteriaiAAC67161; AAC67161; BB_0828.
    GeneIDi1195686.
    KEGGibbu:BB_0828.
    PATRICi20558321. VBIBorBur75917_1220.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000783 Genomic DNA. Translation: AAC67161.1 .
    PIRi C70203.
    RefSeqi NP_212962.1. NC_001318.1.

    3D structure databases

    ProteinModelPortali O51768.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224326.BB0828.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC67161 ; AAC67161 ; BB_0828 .
    GeneIDi 1195686.
    KEGGi bbu:BB_0828.
    PATRICi 20558321. VBIBorBur75917_1220.

    Phylogenomic databases

    eggNOGi COG1754.
    KOi K03168.
    OMAi ANEGYER.
    OrthoDBi EOG6S7XQ9.

    Enzyme and pathway databases

    BioCyci BBUR224326:G9SO-837-MONOMER.

    Family and domain databases

    Gene3Di 1.10.290.10. 1 hit.
    1.10.460.10. 1 hit.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

    Entry informationi

    Entry nameiTOP1_BORBU
    AccessioniPrimary (citable) accession number: O51768
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3