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O51768

- TOP1_BORBU

UniProt

O51768 - TOP1_BORBU

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Protein

DNA topoisomerase 1

Gene

topA

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg(2+) per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi10 – 101Magnesium 1; catalyticUniRule annotation
Sitei34 – 341Interaction with DNAUniRule annotation
Metal bindingi97 – 971Magnesium 1; catalyticUniRule annotation
Metal bindingi97 – 971Magnesium 2UniRule annotation
Metal bindingi99 – 991Magnesium 2UniRule annotation
Sitei152 – 1521Interaction with DNAUniRule annotation
Sitei153 – 1531Interaction with DNAUniRule annotation
Sitei156 – 1561Interaction with DNAUniRule annotation
Sitei161 – 1611Interaction with DNAUniRule annotation
Sitei168 – 1681Interaction with DNAUniRule annotation
Active sitei324 – 3241O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei326 – 3261Interaction with DNAUniRule annotation
Sitei511 – 5111Interaction with DNAUniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBBUR224326:G9SO-837-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:BB_0828
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
ProteomesiUP000001807: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 848848DNA topoisomerase 1PRO_0000145143Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224326.BB0828.

Structurei

3D structure databases

ProteinModelPortaliO51768.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 128125ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni176 – 1816Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1754.
KOiK03168.
OMAiANEGYER.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 1 hit.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O51768-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQNKKLIIVE SPTKAKTIKK FLDESFLVEA CIGHVVDLPN NAKEIPKEYK
60 70 80 90 100
KYEWANISID YNNGFNPIYI IPSNKKPIVS KLKKLVKTIN EIYLATDQDR
110 120 130 140 150
EGETIAFHLK EVLKIKNYKR MIFHEITETA ITESLKNTRN IDMNLVNAGE
160 170 180 190 200
ARRILDRLYG YTISPLLWKK VAYGLSAGRV QSVGLKLLIE KEKTRINFKK
210 220 230 240 250
ANYYSILLQC KHEKKNLLLE AKLEEIDGKN IAEGKDFVNE TGKLKNIAKT
260 270 280 290 300
TIITQDLMIE LEKELKNGQK IELISIETKK IKIPPPKPFT TSTLQQEINK
310 320 330 340 350
RLKIGTKQIM QHAQKLYEHG YITYMRTDSH NIAKIAKDKI TKIIKNKYGK
360 370 380 390 400
EYIEEKDRIY EKEKMAQNAH EAIRPSEIFI PNETIEIESK TAKEIYKIIW
410 420 430 440 450
DRTIISGMKD AIKENIKLTF KYKNLIFRSS FTKIIFDGFL KHTKEQDEHL
460 470 480 490 500
NINFDLIKKG DTFSIVKMKT SEHETKAPFR YTEASLVQKM EKEGIGRPST
510 520 530 540 550
YSTIISTLLE REYAFKLNNT LMPTIKGAAV INLLEKYFPV LIELNFTSNM
560 570 580 590 600
EEKLDKIAIG KLDKIKYLSK FYNGKKGLKD TVMQLEPKID SSEFRTVIES
610 620 630 640 650
QKIENKNSIN YTINIGKYGP YLIFKGHNYS INAKTPLENL YKKDEIEKII
660 670 680 690 700
NEKELKPNIL GVDPLTGLNV IFKNTIYGNI VQLGEDTHAP QEYTKKGKPK
710 720 730 740 750
KLKIIKAKKA STKKIDPENI TLELALKLLS LPKPIGKHPQ TNEQIIAATG
760 770 780 790 800
VFGDYIKTES GSIACSLKKD LKAYDITLDK AISLLNERAN KVGIIVKTIT
810 820 830 840
FSKNKIGNKI YIYKKNDKFY AKIKRKKIDL PDNINLEEIN EKYVFSLL
Length:848
Mass (Da):97,663
Last modified:June 1, 1998 - v1
Checksum:i37DEA5FB1E12FA2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC67161.1.
PIRiC70203.
RefSeqiNP_212962.1. NC_001318.1.

Genome annotation databases

EnsemblBacteriaiAAC67161; AAC67161; BB_0828.
GeneIDi1195686.
KEGGibbu:BB_0828.
PATRICi20558321. VBIBorBur75917_1220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC67161.1 .
PIRi C70203.
RefSeqi NP_212962.1. NC_001318.1.

3D structure databases

ProteinModelPortali O51768.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224326.BB0828.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC67161 ; AAC67161 ; BB_0828 .
GeneIDi 1195686.
KEGGi bbu:BB_0828.
PATRICi 20558321. VBIBorBur75917_1220.

Phylogenomic databases

eggNOGi COG1754.
KOi K03168.
OMAi ANEGYER.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci BBUR224326:G9SO-837-MONOMER.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 1 hit.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

Entry informationi

Entry nameiTOP1_BORBU
AccessioniPrimary (citable) accession number: O51768
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: November 26, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3