Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA topoisomerase 1

Gene

topA

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10Magnesium 1; catalyticUniRule annotation1
Metal bindingi97Magnesium 1; catalyticUniRule annotation1
Metal bindingi97Magnesium 2UniRule annotation1
Metal bindingi99Magnesium 2UniRule annotation1
Active sitei324O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:BB_0828
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliella
Proteomesi
  • UP000001807 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451431 – 848DNA topoisomerase 1Add BLAST848

Proteomic databases

PRIDEiO51768.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei34Interaction with DNAUniRule annotation1
Sitei152Interaction with DNAUniRule annotation1
Sitei153Interaction with DNAUniRule annotation1
Sitei156Interaction with DNAUniRule annotation1
Sitei161Interaction with DNAUniRule annotation1
Sitei168Interaction with DNAUniRule annotation1
Sitei326Interaction with DNAUniRule annotation1
Sitei511Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi224326.BB_0828.

Structurei

3D structure databases

ProteinModelPortaliO51768.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 128ToprimUniRule annotationAdd BLAST125

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni176 – 181Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C73. Bacteria.
COG0550. LUCA.
COG1754. LUCA.
KOiK03168.
OMAiVEACIGH.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 1 hit.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 2 hits.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O51768-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNKKLIIVE SPTKAKTIKK FLDESFLVEA CIGHVVDLPN NAKEIPKEYK
60 70 80 90 100
KYEWANISID YNNGFNPIYI IPSNKKPIVS KLKKLVKTIN EIYLATDQDR
110 120 130 140 150
EGETIAFHLK EVLKIKNYKR MIFHEITETA ITESLKNTRN IDMNLVNAGE
160 170 180 190 200
ARRILDRLYG YTISPLLWKK VAYGLSAGRV QSVGLKLLIE KEKTRINFKK
210 220 230 240 250
ANYYSILLQC KHEKKNLLLE AKLEEIDGKN IAEGKDFVNE TGKLKNIAKT
260 270 280 290 300
TIITQDLMIE LEKELKNGQK IELISIETKK IKIPPPKPFT TSTLQQEINK
310 320 330 340 350
RLKIGTKQIM QHAQKLYEHG YITYMRTDSH NIAKIAKDKI TKIIKNKYGK
360 370 380 390 400
EYIEEKDRIY EKEKMAQNAH EAIRPSEIFI PNETIEIESK TAKEIYKIIW
410 420 430 440 450
DRTIISGMKD AIKENIKLTF KYKNLIFRSS FTKIIFDGFL KHTKEQDEHL
460 470 480 490 500
NINFDLIKKG DTFSIVKMKT SEHETKAPFR YTEASLVQKM EKEGIGRPST
510 520 530 540 550
YSTIISTLLE REYAFKLNNT LMPTIKGAAV INLLEKYFPV LIELNFTSNM
560 570 580 590 600
EEKLDKIAIG KLDKIKYLSK FYNGKKGLKD TVMQLEPKID SSEFRTVIES
610 620 630 640 650
QKIENKNSIN YTINIGKYGP YLIFKGHNYS INAKTPLENL YKKDEIEKII
660 670 680 690 700
NEKELKPNIL GVDPLTGLNV IFKNTIYGNI VQLGEDTHAP QEYTKKGKPK
710 720 730 740 750
KLKIIKAKKA STKKIDPENI TLELALKLLS LPKPIGKHPQ TNEQIIAATG
760 770 780 790 800
VFGDYIKTES GSIACSLKKD LKAYDITLDK AISLLNERAN KVGIIVKTIT
810 820 830 840
FSKNKIGNKI YIYKKNDKFY AKIKRKKIDL PDNINLEEIN EKYVFSLL
Length:848
Mass (Da):97,663
Last modified:June 1, 1998 - v1
Checksum:i37DEA5FB1E12FA2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC67161.1.
PIRiC70203.
RefSeqiNP_212962.1. NC_001318.1.
WP_002657022.1. NC_001318.1.

Genome annotation databases

EnsemblBacteriaiAAC67161; AAC67161; BB_0828.
GeneIDi1195686.
KEGGibbu:BB_0828.
PATRICi20558321. VBIBorBur75917_1220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC67161.1.
PIRiC70203.
RefSeqiNP_212962.1. NC_001318.1.
WP_002657022.1. NC_001318.1.

3D structure databases

ProteinModelPortaliO51768.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224326.BB_0828.

Proteomic databases

PRIDEiO51768.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC67161; AAC67161; BB_0828.
GeneIDi1195686.
KEGGibbu:BB_0828.
PATRICi20558321. VBIBorBur75917_1220.

Phylogenomic databases

eggNOGiENOG4105C73. Bacteria.
COG0550. LUCA.
COG1754. LUCA.
KOiK03168.
OMAiVEACIGH.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 1 hit.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 2 hits.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOP1_BORBU
AccessioniPrimary (citable) accession number: O51768
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.