O51768 (TOP1_BORBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 1
EC=5.99.1.2

Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme

Gene names

Name:	topA
Ordered Locus Names:	BB_0828

Organism

Borrelia burgdorferi (Lyme disease spirochete)

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	848 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the type IA topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 848	848	DNA topoisomerase 1		PRO_0000145143
Regions
	Domain	4 – 125	122	Toprim
Sites
	Active site	324	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O51768 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 37DEA5FB1E12FA2C	FASTA	848	97,663
	10 20 30 40 50 60 MQNKKLIIVE SPTKAKTIKK FLDESFLVEA CIGHVVDLPN NAKEIPKEYK KYEWANISID 70 80 90 100 110 120 YNNGFNPIYI IPSNKKPIVS KLKKLVKTIN EIYLATDQDR EGETIAFHLK EVLKIKNYKR 130 140 150 160 170 180 MIFHEITETA ITESLKNTRN IDMNLVNAGE ARRILDRLYG YTISPLLWKK VAYGLSAGRV 190 200 210 220 230 240 QSVGLKLLIE KEKTRINFKK ANYYSILLQC KHEKKNLLLE AKLEEIDGKN IAEGKDFVNE 250 260 270 280 290 300 TGKLKNIAKT TIITQDLMIE LEKELKNGQK IELISIETKK IKIPPPKPFT TSTLQQEINK 310 320 330 340 350 360 RLKIGTKQIM QHAQKLYEHG YITYMRTDSH NIAKIAKDKI TKIIKNKYGK EYIEEKDRIY 370 380 390 400 410 420 EKEKMAQNAH EAIRPSEIFI PNETIEIESK TAKEIYKIIW DRTIISGMKD AIKENIKLTF 430 440 450 460 470 480 KYKNLIFRSS FTKIIFDGFL KHTKEQDEHL NINFDLIKKG DTFSIVKMKT SEHETKAPFR 490 500 510 520 530 540 YTEASLVQKM EKEGIGRPST YSTIISTLLE REYAFKLNNT LMPTIKGAAV INLLEKYFPV 550 560 570 580 590 600 LIELNFTSNM EEKLDKIAIG KLDKIKYLSK FYNGKKGLKD TVMQLEPKID SSEFRTVIES 610 620 630 640 650 660 QKIENKNSIN YTINIGKYGP YLIFKGHNYS INAKTPLENL YKKDEIEKII NEKELKPNIL 670 680 690 700 710 720 GVDPLTGLNV IFKNTIYGNI VQLGEDTHAP QEYTKKGKPK KLKIIKAKKA STKKIDPENI 730 740 750 760 770 780 TLELALKLLS LPKPIGKHPQ TNEQIIAATG VFGDYIKTES GSIACSLKKD LKAYDITLDK 790 800 810 820 830 840 AISLLNERAN KVGIIVKTIT FSKNKIGNKI YIYKKNDKFY AKIKRKKIDL PDNINLEEIN EKYVFSLL

References

[1]

"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000783 Genomic DNA. Translation: AAC67161.1.
PIR	C70203.
RefSeq	NP_212962.1. NC_001318.1.
3D structure databases
ProteinModelPortal	O51768.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000008495; EBBORP00000007825; EBBORG00000008494.
GeneID	1195686.
GenomeReviews	Gene locus BB_0828 in contig AE000783_GR.
KEGG	bbu:BB0828.
NMPDR	fig|224326.1.peg.1212.
PATRIC	20558321. VBIBorBur75917_1220.
TIGR	BB_0828.
Phylogenomic databases
GeneTree	EBGT00050000007103.
HOGENOM	HBG512935.
OMA	KKVMPRL.
PhylomeDB	O51768.
ProtClustDB	CLSK507867.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0828-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 1 hit. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_BORBU

Accession

Primary (citable) accession number: O51768

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program