Reviewed, UniProtKB/Swiss-Prot O51757 (MURC_BORBU)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	BB_0817

Organism

Borrelia burgdorferi (Lyme disease spirochete) [Complete proteome] [HAMAP]

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182061
Regions
	Nucleotide binding	121 – 127	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O51757-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: BE486CAB970D82D3	FASTA	468	53,480
	10 20 30 40 50 60 MKVDFDDLNN IFFVGIKGSG ACSLACFLNS KGYCVEGVDV SDKFYTDEIL SNNKISYYDN 70 80 90 100 110 120 IYEFSLKQLD RSFDLIVYSS AYNKDGLQVL LEAKELNIPI LSYPEALGEL SRKYYSIGIA 130 140 150 160 170 180 GSHGKTTTTA FLGVLFNKLG LNPNVIVGSS VKDFKDNSAI AGISNIFIVE TCEYKKHFLN 190 200 210 220 230 240 FSPNMLILTN VDYEHVDFFK NYEALEEAFL QYINNLKKNG ILIINSDDNN LLKIKRQINR 250 260 270 280 290 300 KDISIFSYGS GDLSDFQISN IAVRSEYFCF SFLGLLNVEL KTVLFHNVLN FSAALLALNL 310 320 330 340 350 360 FLESNGKSIF DFEEAIKRIA KNYSGIKRRV EVVKEENGVI YMDDYAHHPR EIKNTLFGIK 370 380 390 400 410 420 NFYKNKRIIL DFMPHTFTRT KEFFADFVEV LSAADILILH NIYLSNRENF NPDELSVKLF 430 440 450 460 LNIKKINKNT YFFKDVKDSI NFIKSLLISG DLFITMGAGN NFILHDFL

References

[1]

"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000783 Genomic DNA. Translation: AAC67166.1.
PIR	H70201.
RefSeq	NP_212951.1.
3D structure databases
HSSP	HSSP built from PDB template 1GQQ based on UniProtKB P45066.
ModBase	Search...
Genome annotation databases
GeneID	1195675.
GenomeReviews	Gene locus BB_0817 in contig AE000783_GR.
KEGG	bbu:BB0817.
NMPDR	fig|224326.1.peg.1201.
TIGR	BB_0817.
Phylogenomic databases
HOGENOM	O51757.
OMA	EWMVVEA.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0817-MON.
BRENDA	6.3.2.8. 142596.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BORBU

Accession

Primary (citable) accession number: O51757

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	November 3, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)