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Protein

Coenzyme A biosynthesis bifunctional protein CoaBC

Gene

coaBC

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine (By similarity).By similarity

Catalytic activityi

N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO2.
CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-((R)-4'-phosphopantothenoyl)-L-cysteine.

Cofactori

FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes CoA from (R)-pantothenate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Type III pantothenate kinase (coaX)
  2. Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  3. Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  4. Phosphopantetheine adenylyltransferase (coaD)
  5. Dephospho-CoA kinase (coaE)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei123SubstrateBy similarity1
Active sitei156Proton donorBy similarity1
Binding sitei277CTPBy similarity1
Binding sitei287CTPBy similarity1
Binding sitei338CTPBy similarity1
Binding sitei342CTPBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Ligase, Lyase
LigandFlavoprotein, FMN

Enzyme and pathway databases

UniPathwayiUPA00241; UER00353.
UPA00241; UER00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme A biosynthesis bifunctional protein CoaBC
Alternative name(s):
DNA/pantothenate metabolism flavoprotein
Including the following 2 domains:
Phosphopantothenoylcysteine decarboxylase (EC:4.1.1.36)
Short name:
PPCDC
Alternative name(s):
CoaC
Phosphopantothenate--cysteine ligase (EC:6.3.2.5)
Alternative name(s):
CoaB
PPC synthetase
Short name:
PPCS
Phosphopantothenoylcysteine synthase
Gene namesi
Name:coaBC
Synonyms:dfp
Ordered Locus Names:BB_0812
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliella
Proteomesi
  • UP000001807 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002326921 – 390Coenzyme A biosynthesis bifunctional protein CoaBCAdd BLAST390

Proteomic databases

PRIDEiO51752.

Interactioni

Subunit structurei

Homododecamer, the CoaB domains form homodimers.

Protein-protein interaction databases

STRINGi224326.BB_0812.

Structurei

3D structure databases

ProteinModelPortaliO51752.
SMRiO51752.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 186Phosphopantothenoylcysteine decarboxylaseAdd BLAST186
Regioni187 – 390Phosphopantothenate--cysteine ligaseAdd BLAST204

Sequence similaritiesi

In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.Curated
In the C-terminal section; belongs to the PPC synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105CJS. Bacteria.
COG0452. LUCA.
KOiK13038.
OMAiVRFIGNH.

Family and domain databases

Gene3Di3.40.50.10300. 1 hit.
3.40.50.1950. 1 hit.
InterProiView protein in InterPro
IPR035929. CoaB-like_sf.
IPR005252. CoaBC.
IPR007085. DNA/pantothenate-metab_flavo_C.
IPR036551. Flavin_trans-like.
IPR003382. Flavoprotein.
PfamiView protein in Pfam
PF04127. DFP. 1 hit.
PF02441. Flavoprotein. 1 hit.
SUPFAMiSSF102645. SSF102645. 1 hit.
SSF52507. SSF52507. 1 hit.
TIGRFAMsiTIGR00521. coaBC_dfp. 1 hit.

Sequencei

Sequence statusi: Complete.

O51752-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKNKHILIG ICGGIASYKS VYIVSSLVKL GYKVKVIMTQ NATKFITPLT
60 70 80 90 100
LETISKNKII TNLWDLDHNE VEHIKIAKWA HLILVIPATY NTISKIASGI
110 120 130 140 150
ADDALTTIIS ASTAPTYFAI AMNNIMYSNP ILKENIKKLK TYNYKFIEPD
160 170 180 190 200
KGFLACSSNA LGRLKNEDKI IKIILNEFNQ KDYLKNKKIL ITASRTEELI
210 220 230 240 250
DPIRYFSNTS TGKMGFCLAQ EAVKLGAQVT IITGPTNEND PEGVNIIKIK
260 270 280 290 300
TAMEMYKEAL KIYNKFEIII GAAAVADFKP KHIFNSKIKK NKINRLYIKL
310 320 330 340 350
VKNPDIIQHI GHNKLKNQIV IGFCAENSKN LIQKAKEKLK KKNLDFIIAN
360 370 380 390
ELKYFGSKLN KVYIINKQSI KELPEMEKSE VAKEILKILY
Length:390
Mass (Da):44,144
Last modified:June 1, 1998 - v1
Checksum:i66868FAB172DE300
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC67145.1.
PIRiC70201.
RefSeqiNP_212946.1. NC_001318.1.
WP_010889826.1. NC_001318.1.

Genome annotation databases

EnsemblBacteriaiAAC67145; AAC67145; BB_0812.
GeneIDi1195670.
KEGGibbu:BB_0812.
PATRICifig|224326.49.peg.1204.

Similar proteinsi

Entry informationi

Entry nameiCOABC_BORBU
AccessioniPrimary (citable) accession number: O51752
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: October 25, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families