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Reviewed, UniProtKB/Swiss-Prot O51749 (TGT_BORBU)

Last modified June 16, 2009. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme
Gene names
Name: tgt
Ordered Locus Names: BB_0809
OrganismBorrelia burgdorferi (Lyme disease spirochete) [Complete proteome] [HAMAP]
Taxonomic identifier139 [NCBI]
Taxonomic lineageBacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

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Protein attributes

Sequence length375 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.

Catalytic activity

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168

[tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168

Cofactor

Binds 1 zinc ion per subunit By similarity.

Pathway

tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00168

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

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Ontologies

Keywords
   Biological processQueuosine biosynthesis
tRNA processing
   LigandMetal-binding
Zinc
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processqueuosine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Molecular functionqueuine tRNA-ribosyltransferase activity

Inferred from electronic annotation. Source: HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 375375Queuine tRNA-ribosyltransferase HAMAP MF_00168
PRO_0000135453

Sites

Active site901Nucleophile By similarity
Metal binding3051Zinc By similarity
Metal binding3071Zinc By similarity
Metal binding3101Zinc By similarity
Metal binding3361Zinc By similarity
Binding site911Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
O51749-1 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 7D7A554644439763

FASTA37543,199
        10         20         30         40         50         60 
MFSVIKNDKH FNARVGFLNL PHGRVDIPCF MPVGTLGAMK GLKHAVLEKL ECNLMLANTY 

        70         80         90        100        110        120 
HLYLRLGIKT VEKYVGLHNF TIWNKNFLTD SGGFRVFSFS DLRKIDLKGV HFKSHIDGSY 

       130        140        150        160        170        180 
HYFTSEGIFA MQEIFGSDII MPLDICSSYG IDYNEANLYT NITTNWASST FKSSKNRKEG 

       190        200        210        220        230        240 
YNGLLFLITQ GNFFKDLRKR SINDILELDS PGIAIGGISV GEPREKYLEI LEYSFLLIPK 

       250        260        270        280        290        300 
EKPRYVMGIG TPHYILNAIY YGIDIFDCFN PARITRHGSL LTDNGIMCIG RKEYKDDTSK 

       310        320        330        340        350        360 
VEKNCICTLC KRYSRGYLRH LIKSKELFGI VLASEHNIHY MFRLISKIRA AILNDDFLNF 

       370 
RTSYLKKYEE ENFDE

« Hide

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Cross-references

Sequence databases

AE000783 Genomic DNA. Translation: AAC67147.1.
PIRH70200.
RefSeqNP_212943.1.

3D structure databases

HSSPHSSP built from PDB template 1K4G based on UniProtKB P28720.
ModBaseSearch...

Genome annotation databases

GeneID1195667.
GenomeReviewsGene locus BB_0809 in contig AE000783_GR.
KEGGbbu:BB0809.
NMPDRfig|224326.1.peg.1193.
TIGRBB_0809.

Phylogenomic databases

HOGENOMO51749.
OMAO51749. ECVRLPA.

Enzyme and pathway databases

BioCycBBUR224326:BB_0809-MON.
BRENDA2.4.2.29. 142596.

Family and domain databases

HAMAPMF_00168.
[Tree]
InterProIPR004803. QtRNA_ribo_trans.
IPR002616. tRNA_ribo_trans.
[Graphical view]
Gene3DG3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHERPTHR11962. tRNA_ribo_trans. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameTGT_BORBU
AccessionPrimary (citable) accession number: O51749
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents