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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).UniRule annotation

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi278ZincUniRule annotation1
Metal bindingi329Zinc; via tele nitrogenUniRule annotation1
Metal bindingi455Zinc; via pros nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligaseUniRule annotation (EC:6.1.1.3UniRule annotation)
Alternative name(s):
Threonyl-tRNA synthetaseUniRule annotation
Short name:
ThrRSUniRule annotation
Gene namesi
Name:thrSUniRule annotation
Ordered Locus Names:BB_0720
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliella
Proteomesi
  • UP000001807 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001009451 – 581Threonine--tRNA ligaseAdd BLAST581

Proteomic databases

PRIDEiO51662.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO51662.
SMRiO51662.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni185 – 478CatalyticUniRule annotationAdd BLAST294

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

OMAiFYYDFAY.

Family and domain databases

CDDicd00771. ThrRS_core. 1 hit.
Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth. 1 hit.
InterProiView protein in InterPro
IPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR033728. ThrRS_core.
IPR012947. tRNA_SAD.
PANTHERiPTHR11451. PTHR11451. 1 hit.
PfamiView protein in Pfam
PF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiView protein in SMART
SM00863. tRNA_SAD. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiView protein in PROSITE
PS50862. AA_TRNA_LIGASE_II. 1 hit.

Sequencei

Sequence statusi: Complete.

O51662-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKDLDKEDI LYKKRHSIAH VMAEAVLDLF PNTKIAIGPP IKDGFYYDFE
60 70 80 90 100
FKKQITEDSL LDIENRMREI LKTGSSFEKE IISVEQALEI FKDEPYKIDL
110 120 130 140 150
IKNFDLQNEV SIYKSHNFVD LCRGPHVENM NKIDPKAFKL TSIAGAYWRG
160 170 180 190 200
SEKNPMLTRI YGTLWNNEKE LRSYLNLREE IKKRDHRKLG KELDLFSIHE
210 220 230 240 250
EIGPGLVFFH PNGAKIRALI EDFWREEHSK NGYDILFTPH IGKSWLWQTS
260 270 280 290 300
GHLDFYKDSM FEKIEMDKSD YYLKPMNCPF HIAIYNTGKH SYRDLPFRWA
310 320 330 340 350
ELGTVYRYEK IGALHGMMRA RGFTQDDAHI ICTHSQVLDE IKEVLRFAIY
360 370 380 390 400
MWSKFGFSNP KAYLSTKPDK SVGNDSDWEM SLKVLEETLS DFEVPYEIDK
410 420 430 440 450
GGGAFYGPKI DLKIVDSLER EWQMSTIQFD FNLPERFNMT YTAEDGKEKR
460 470 480 490 500
PFMIHRALLG SIERFFGILV EHYGGAFPLW LSPVQVVIIP VNNIVEDYAI
510 520 530 540 550
KVFNKFKNEG IRIKLDNSSS RMNAKIREYQ AKKIPYMFII GEREATEERI
560 570 580
SIRTRTNEQI NGMKLDEALK FILFKIRDKE I
Length:581
Mass (Da):68,172
Last modified:June 1, 1998 - v1
Checksum:i52CEC7B50BEFC169
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. No translation available.
PIRiG70189.
RefSeqiWP_002665762.1. NC_001318.1.
YP_008686586.1. NC_001318.1.

Genome annotation databases

GeneIDi1195574.
KEGGibbu:BB_0720.
PATRICifig|224326.49.peg.1111.

Similar proteinsi

Entry informationi

Entry nameiSYT_BORBU
AccessioniPrimary (citable) accession number: O51662
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: September 27, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families