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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).UniRule annotation

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi278ZincUniRule annotation1
Metal bindingi329Zinc; via tele nitrogenUniRule annotation1
Metal bindingi455Zinc; via pros nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligaseUniRule annotation (EC:6.1.1.3UniRule annotation)
Alternative name(s):
Threonyl-tRNA synthetaseUniRule annotation
Short name:
ThrRSUniRule annotation
Gene namesi
Name:thrSUniRule annotation
Ordered Locus Names:BB_0720
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliella
Proteomesi
  • UP000001807 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001009451 – 581Threonine--tRNA ligaseAdd BLAST581

Proteomic databases

PRIDEiO51662

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO51662
SMRiO51662
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni185 – 478CatalyticUniRule annotationAdd BLAST294

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

KOiK01868
OMAiFYYDFAY

Family and domain databases

CDDicd00771 ThrRS_core, 1 hit
Gene3Di3.40.50.800, 1 hit
HAMAPiMF_00184 Thr_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR002320 Thr-tRNA-ligase_IIa
IPR018163 Thr/Ala-tRNA-synth_IIc_edit
IPR033728 ThrRS_core
IPR012947 tRNA_SAD
PANTHERiPTHR11451 PTHR11451, 1 hit
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PF07973 tRNA_SAD, 1 hit
PRINTSiPR01047 TRNASYNTHTHR
SMARTiView protein in SMART
SM00863 tRNA_SAD, 1 hit
SUPFAMiSSF55186 SSF55186, 1 hit
TIGRFAMsiTIGR00418 thrS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

O51662-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKDLDKEDI LYKKRHSIAH VMAEAVLDLF PNTKIAIGPP IKDGFYYDFE
60 70 80 90 100
FKKQITEDSL LDIENRMREI LKTGSSFEKE IISVEQALEI FKDEPYKIDL
110 120 130 140 150
IKNFDLQNEV SIYKSHNFVD LCRGPHVENM NKIDPKAFKL TSIAGAYWRG
160 170 180 190 200
SEKNPMLTRI YGTLWNNEKE LRSYLNLREE IKKRDHRKLG KELDLFSIHE
210 220 230 240 250
EIGPGLVFFH PNGAKIRALI EDFWREEHSK NGYDILFTPH IGKSWLWQTS
260 270 280 290 300
GHLDFYKDSM FEKIEMDKSD YYLKPMNCPF HIAIYNTGKH SYRDLPFRWA
310 320 330 340 350
ELGTVYRYEK IGALHGMMRA RGFTQDDAHI ICTHSQVLDE IKEVLRFAIY
360 370 380 390 400
MWSKFGFSNP KAYLSTKPDK SVGNDSDWEM SLKVLEETLS DFEVPYEIDK
410 420 430 440 450
GGGAFYGPKI DLKIVDSLER EWQMSTIQFD FNLPERFNMT YTAEDGKEKR
460 470 480 490 500
PFMIHRALLG SIERFFGILV EHYGGAFPLW LSPVQVVIIP VNNIVEDYAI
510 520 530 540 550
KVFNKFKNEG IRIKLDNSSS RMNAKIREYQ AKKIPYMFII GEREATEERI
560 570 580
SIRTRTNEQI NGMKLDEALK FILFKIRDKE I
Length:581
Mass (Da):68,172
Last modified:June 1, 1998 - v1
Checksum:i52CEC7B50BEFC169
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA No translation available.
PIRiG70189
RefSeqiWP_002665762.1, NC_001318.1
YP_008686586.1, NC_001318.1

Genome annotation databases

GeneIDi1195574
KEGGibbu:BB_0720
PATRICifig|224326.49.peg.1111

Similar proteinsi

Entry informationi

Entry nameiSYT_BORBU
AccessioniPrimary (citable) accession number: O51662
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: April 25, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health