O51641 (TRMD_BORBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BB_0698

Organism

Borrelia burgdorferi (Lyme disease spirochete)

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060339
Regions
	Region	130 – 135	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	110	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	O51641 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 4533FBED8706B144	FASTA	239	27,179
	10 20 30 40 50 60 MKFTVLSLFP AIIKPFFENS IMKKAINKGI VSFELVDVRD FSKDKHKRCD DLPYGGGAGM 70 80 90 100 110 120 VLKAEPISFA LEHVESAKKT TIFLSPSGIK YSQELAYSLS KREEIVIICG RYEGIDQRII 130 140 150 160 170 180 DLYVDFEISI GDYVLSSGEI AALVLIDSVY RLLDGVINPN SLLEESFGVK NGLLEYPHYT 190 200 210 220 230 RPYDFKGIKV PEVLLSGHHA NIKNWRLVKA REKTKKNRYD LYLKYLEIIG EDNGFDKKN

References

[1]

"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000783 Genomic DNA. Translation: AAC67045.1.
PIR	A70187.
RefSeq	NP_212832.1. NC_001318.1.
3D structure databases
ProteinModelPortal	O51641.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000008812; EBBORP00000008142; EBBORG00000008811.
GeneID	1195551.
GenomeReviews	Gene locus BB_0698 in contig AE000783_GR.
KEGG	bbu:BB0698.
NMPDR	fig|224326.1.peg.1082.
PATRIC	20558047. VBIBorBur75917_1089.
TIGR	BB_0698.
Phylogenomic databases
GeneTree	EBGT00050000007285.
HOGENOM	HBG285805.
OMA	NRYDLYL.
PhylomeDB	O51641.
ProtClustDB	PRK00026.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0698-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BORBU

Accession

Primary (citable) accession number: O51641

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program