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Protein

RecBCD enzyme subunit RecB

Gene

recB

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA.UniRule annotation

Catalytic activityi

Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi939Magnesium; via tele nitrogenUniRule annotation1
Metal bindingi1052MagnesiumUniRule annotation1
Active sitei1065For nuclease activityBy similarity1
Metal bindingi1065MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 25ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Exonuclease, Helicase, Hydrolase, Nuclease
Biological processDNA damage, DNA repair
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RecBCD enzyme subunit RecBUniRule annotation (EC:3.1.11.5UniRule annotation)
Alternative name(s):
Exonuclease V subunit RecBUniRule annotation
Short name:
ExoV subunit RecBUniRule annotation
Helicase/nuclease RecBCD subunit RecBUniRule annotation
Gene namesi
Name:recBUniRule annotation
Ordered Locus Names:BB_0633
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliella
Proteomesi
  • UP000001807 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001020391 – 1169RecBCD enzyme subunit RecBAdd BLAST1169

Proteomic databases

PRIDEiO51578

Interactioni

Subunit structurei

Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding. Interacts with RecA.UniRule annotation

Protein-protein interaction databases

STRINGi224326.BB_0633

Structurei

3D structure databases

ProteinModelPortaliO51578
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 436UvrD-like helicase ATP-bindingUniRule annotationAdd BLAST436
Domaini459 – 746UvrD-like helicase C-terminalUniRule annotationAdd BLAST288

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 843DNA-binding and helicase activity, interacts with RecCUniRule annotationAdd BLAST843
Regioni883 – 1169Nuclease activity, interacts with RecD and RecAUniRule annotationAdd BLAST287

Domaini

The N-terminal DNA-binding domain is a ssDNA-dependent ATPase and has ATP-dependent 3'-5' helicase function. This domain interacts with RecC.UniRule annotation
The C-terminal domain has nuclease activity and interacts with RecD. It interacts with RecA, facilitating its loading onto ssDNA.UniRule annotation

Sequence similaritiesi

Belongs to the helicase family. UvrD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107X59 Bacteria
COG1074 LUCA
KOiK03582
OMAiKQSIYRW

Family and domain databases

Gene3Di3.90.320.10, 1 hit
HAMAPiMF_01485 RecB, 1 hit
InterProiView protein in InterPro
IPR014017 DNA_helicase_UvrD-like_C
IPR000212 DNA_helicase_UvrD/REP
IPR011604 Exonuc_phg/RecB_C
IPR027417 P-loop_NTPase
IPR038726 PDDEXK_AddAB-type
IPR004586 RecB
IPR011335 Restrct_endonuc-II-like
IPR014016 UvrD-like_ATP-bd
IPR034739 UvrD/AddA_N
PANTHERiPTHR11070 PTHR11070, 1 hit
PTHR11070:SF23 PTHR11070:SF23, 1 hit
PfamiView protein in Pfam
PF12705 PDDEXK_1, 1 hit
PF00580 UvrD-helicase, 1 hit
PF13361 UvrD_C, 1 hit
SUPFAMiSSF52540 SSF52540, 4 hits
SSF52980 SSF52980, 1 hit
TIGRFAMsiTIGR00609 recB, 1 hit
PROSITEiView protein in PROSITE
PS51198 UVRD_HELICASE_ATP_BIND, 1 hit
PS51217 UVRD_HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

O51578-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKILEKIQN NTTILIEASA GTGKTHILEN VVINLIKTKL YSINEILVLT
60 70 80 90 100
FTKKATEEMH TRILKVIENA YSNSKTNEIL KEAYEQSKKL FISTINKFAL
110 120 130 140 150
HALNNFQIET ENYSKYKPKE KFSKEIDEIV YDFLRKSDSL IQALDIKDYE
160 170 180 190 200
LKVFKSDAKK TEEIVLKIKK AYERDTTQEL GDWLKTQTAF ENILLKKEEL
210 220 230 240 250
IKDYNKIIED LDKMTKDEIL SFYNKHIQTG KLEIEYSKEN DIFKIAETLL
260 270 280 290 300
KNKFFSTLIE KETKKNSKLS PKELKIKNDL ICLGINIKHE KYKSEDNRNK
310 320 330 340 350
NRNNLKQYVI LKVEYKILKY IEKELKKTIK STNTIDQNYI ISNLKNYLKS
360 370 380 390 400
EDKKLLNAIK NRYKIILIDE AQDLSLIQIE IFKILKTAGI KLIFIADPKQ
410 420 430 440 450
IIYSFRKADI SFYNKEIKNK INTDARIVLK INHRSSKKLI GPLNKIFNNI
460 470 480 490 500
YNNAIADEIE KIDFTNSLPN QKNDNNKIVI NGQEIEGINI ITTNTESEED
510 520 530 540 550
IYQKTALTIK YLLAYGKIAE NNKIRNIKMQ DIKVLCRGKN EINLIDKALK
560 570 580 590 600
KEQIQTNKTQ EKFLKTKEFS EIFYIIKCLD RKQSFKTLNY ILSSKILNVP
610 620 630 640 650
WNLQRILIKQ DKICLIEEFI ENIIVLLEKN EITLINAINK ITFEKNLWIK
660 670 680 690 700
IANITKDQKI IEWAKNKINY KGLLIKEGKL ENLKTYETTL EIISKIYHKE
710 720 730 740 750
QNIQSLISTL ESLIINEEPE EIEEKINNIN NDNESIELMT IHKSKGLGMN
760 770 780 790 800
IVFLLNTTPI ENSNFFSKKN QFYKFYQDGK IEYDFFKLEE NKKYARLKIL
810 820 830 840 850
SEEKNIFYVG ATRAKFALFI IKINSITSKL LEIAKIFTID DIKHDFNIHE
860 870 880 890 900
FIGQKRFNKK KYNTNVNTKL IPPKPIIKNM FKKEYTSSFS SLTAQAHHKE
910 920 930 940 950
FYENYDFKNI NYEKETELDY EPGLEETLPK GKDIGNILHA AMEEIIFSTA
960 970 980 990 1000
KDTFDNFKKN NIEIIEKQIQ KINSNLNTIE IQNSLAKMIY NILTYNIRAI
1010 1020 1030 1040 1050
NTRLCDIEEL QKEMEFLIKI NPEFQKQKYL FDKHFEDLHI KLSDGYLKGI
1060 1070 1080 1090 1100
VDLIFKANNK IYILDYKTNY LGKNKEDYNI TNLENTIKKE YYDLQYKIYA
1110 1120 1130 1140 1150
LGIKKILFKN KKEYNQKFGG IIYLFTRAFE DNIECLKSKF ENGIYFNLPK
1160
FNDVDLDKII LELGIKRHL
Length:1,169
Mass (Da):137,829
Last modified:June 1, 1998 - v1
Checksum:iB61D63C1C959B91F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA Translation: AAC66981.1
PIRiH70178
RefSeqiNP_212767.1, NC_001318.1
WP_010889786.1, NC_001318.1

Genome annotation databases

EnsemblBacteriaiAAC66981; AAC66981; BB_0633
GeneIDi1195485
KEGGibbu:BB_0633
PATRICifig|224326.49.peg.1023

Similar proteinsi

Entry informationi

Entry nameiRECB_BORBU
AccessioniPrimary (citable) accession number: O51578
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: April 25, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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