Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi27ZincBy similarity1
Metal bindingi221ZincBy similarity1
Metal bindingi246ZincBy similarity1
Metal bindingi250ZincBy similarity1
Binding sitei281ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligase (EC:6.1.1.16)
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name:
CysRS
Gene namesi
Name:cysS
Ordered Locus Names:BB_0599
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliella
Proteomesi
  • UP000001807 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001593591 – 480Cysteine--tRNA ligaseAdd BLAST480

Proteomic databases

PRIDEiO51545.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi224326.BB_0599.

Structurei

Secondary structure

1480
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Turni8 – 10Combined sources3
Beta strandi11 – 16Combined sources6
Beta strandi22 – 26Combined sources5
Helixi37 – 56Combined sources20
Beta strandi60 – 67Combined sources8
Helixi94 – 111Combined sources18
Beta strandi118 – 122Combined sources5
Helixi123 – 125Combined sources3
Helixi127 – 139Combined sources13
Beta strandi143 – 146Combined sources4
Beta strandi149 – 152Combined sources4
Helixi154 – 156Combined sources3
Turni158 – 164Combined sources7
Beta strandi190 – 195Combined sources6
Beta strandi212 – 215Combined sources4
Helixi217 – 228Combined sources12
Turni229 – 231Combined sources3
Beta strandi235 – 239Combined sources5
Helixi240 – 242Combined sources3
Turni243 – 245Combined sources3
Helixi246 – 258Combined sources13
Beta strandi264 – 269Combined sources6
Beta strandi276 – 278Combined sources3
Helixi289 – 294Combined sources6
Helixi299 – 307Combined sources9
Helixi319 – 341Combined sources23
Helixi345 – 352Combined sources8
Helixi361 – 375Combined sources15
Helixi380 – 392Combined sources13
Beta strandi394 – 396Combined sources3
Helixi398 – 412Combined sources15
Helixi416 – 424Combined sources9
Helixi433 – 447Combined sources15
Helixi451 – 463Combined sources13
Beta strandi476 – 479Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SP1X-ray2.55A/B1-480[»]
ProteinModelPortaliO51545.
SMRiO51545.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi29 – 39"HIGH" regionAdd BLAST11
Motifi278 – 282"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
KOiK01883.
OMAiAPYKRSP.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth. 1 hit.
InterProiIPR015803. Cys-tRNA-ligase.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

O51545-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILKLYNTRT KDFSELTNFE NVKVYACGPT VYNYAHIGNF RTYIFGDLLI
60 70 80 90 100
KTLRFLGYKV NYAMNITDIG HLTGDLDDGE DKVAKTAREK GLTVYEISEF
110 120 130 140 150
FTEAFFNDCR KLNIVYPDKV LVASKHIPIM IEVVKILEEK KITYFSNGNV
160 170 180 190 200
YFDTSCFKSY GEMAGIDLID KDMTLPRVDV DKFKRNKTDF VLWFTNSKFK
210 220 230 240 250
DQEMKWDSPW GFGYPSWHLE CAAMNLEYFK DALDIHLGGV DHIGVHHINE
260 270 280 290 300
IAIAECFLNK KWCDVFVHGE FLIMDYNKMS KSRGNFITVK DLEDQNFSPL
310 320 330 340 350
DFRYLCLTSH YRNQLKFSLD NLQASKIARE NLINKLSYFY ESLDPVDLNT
360 370 380 390 400
LNKDLKNFGF SVEKEYYDSF VEKISFDLNV AQGLALLWEI IKSDNLSFVS
410 420 430 440 450
KLRLAFIFDE IMSLNLREEI LKNLQNHDVV IDENMKALIE ERRIAKCEKN
460 470 480
FKRADEIRDF FAKKGFVLVD TKEGTKVKRG
Length:480
Mass (Da):56,022
Last modified:June 1, 1998 - v1
Checksum:i8C689854C8AFA60F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC66952.1.
PIRiF70174.
RefSeqiNP_212733.1. NC_001318.1.
WP_002662611.1. NC_001318.1.

Genome annotation databases

EnsemblBacteriaiAAC66952; AAC66952; BB_0599.
GeneIDi1195446.
KEGGibbu:BB_0599.
PATRICi20557831. VBIBorBur75917_0990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC66952.1.
PIRiF70174.
RefSeqiNP_212733.1. NC_001318.1.
WP_002662611.1. NC_001318.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SP1X-ray2.55A/B1-480[»]
ProteinModelPortaliO51545.
SMRiO51545.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224326.BB_0599.

Proteomic databases

PRIDEiO51545.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC66952; AAC66952; BB_0599.
GeneIDi1195446.
KEGGibbu:BB_0599.
PATRICi20557831. VBIBorBur75917_0990.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
KOiK01883.
OMAiAPYKRSP.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth. 1 hit.
InterProiIPR015803. Cys-tRNA-ligase.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYC_BORBU
AccessioniPrimary (citable) accession number: O51545
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.