Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi27 – 271ZincBy similarity
Metal bindingi221 – 2211ZincBy similarity
Metal bindingi246 – 2461ZincBy similarity
Metal bindingi250 – 2501ZincBy similarity
Binding sitei281 – 2811ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBBUR224326:G9SO-601-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligase (EC:6.1.1.16)
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name:
CysRS
Gene namesi
Name:cysS
Ordered Locus Names:BB_0599
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliella
Proteomesi
  • UP000001807 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480Cysteine--tRNA ligasePRO_0000159359Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi224326.BB_0599.

Structurei

Secondary structure

1
480
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 64Combined sources
Turni8 – 103Combined sources
Beta strandi11 – 166Combined sources
Beta strandi22 – 265Combined sources
Helixi37 – 5620Combined sources
Beta strandi60 – 678Combined sources
Helixi94 – 11118Combined sources
Beta strandi118 – 1225Combined sources
Helixi123 – 1253Combined sources
Helixi127 – 13913Combined sources
Beta strandi143 – 1464Combined sources
Beta strandi149 – 1524Combined sources
Helixi154 – 1563Combined sources
Turni158 – 1647Combined sources
Beta strandi190 – 1956Combined sources
Beta strandi212 – 2154Combined sources
Helixi217 – 22812Combined sources
Turni229 – 2313Combined sources
Beta strandi235 – 2395Combined sources
Helixi240 – 2423Combined sources
Turni243 – 2453Combined sources
Helixi246 – 25813Combined sources
Beta strandi264 – 2696Combined sources
Beta strandi276 – 2783Combined sources
Helixi289 – 2946Combined sources
Helixi299 – 3079Combined sources
Helixi319 – 34123Combined sources
Helixi345 – 3528Combined sources
Helixi361 – 37515Combined sources
Helixi380 – 39213Combined sources
Beta strandi394 – 3963Combined sources
Helixi398 – 41215Combined sources
Helixi416 – 4249Combined sources
Helixi433 – 44715Combined sources
Helixi451 – 46313Combined sources
Beta strandi476 – 4794Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SP1X-ray2.55A/B1-480[»]
ProteinModelPortaliO51545.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi29 – 3911"HIGH" regionAdd
BLAST
Motifi278 – 2825"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
KOiK01883.
OMAiAPYKRSP.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth. 1 hit.
InterProiIPR015803. Cys-tRNA-ligase.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

O51545-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILKLYNTRT KDFSELTNFE NVKVYACGPT VYNYAHIGNF RTYIFGDLLI
60 70 80 90 100
KTLRFLGYKV NYAMNITDIG HLTGDLDDGE DKVAKTAREK GLTVYEISEF
110 120 130 140 150
FTEAFFNDCR KLNIVYPDKV LVASKHIPIM IEVVKILEEK KITYFSNGNV
160 170 180 190 200
YFDTSCFKSY GEMAGIDLID KDMTLPRVDV DKFKRNKTDF VLWFTNSKFK
210 220 230 240 250
DQEMKWDSPW GFGYPSWHLE CAAMNLEYFK DALDIHLGGV DHIGVHHINE
260 270 280 290 300
IAIAECFLNK KWCDVFVHGE FLIMDYNKMS KSRGNFITVK DLEDQNFSPL
310 320 330 340 350
DFRYLCLTSH YRNQLKFSLD NLQASKIARE NLINKLSYFY ESLDPVDLNT
360 370 380 390 400
LNKDLKNFGF SVEKEYYDSF VEKISFDLNV AQGLALLWEI IKSDNLSFVS
410 420 430 440 450
KLRLAFIFDE IMSLNLREEI LKNLQNHDVV IDENMKALIE ERRIAKCEKN
460 470 480
FKRADEIRDF FAKKGFVLVD TKEGTKVKRG
Length:480
Mass (Da):56,022
Last modified:June 1, 1998 - v1
Checksum:i8C689854C8AFA60F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC66952.1.
PIRiF70174.
RefSeqiNP_212733.1. NC_001318.1.
WP_002662611.1. NC_001318.1.

Genome annotation databases

EnsemblBacteriaiAAC66952; AAC66952; BB_0599.
GeneIDi1195446.
KEGGibbu:BB_0599.
PATRICi20557831. VBIBorBur75917_0990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAC66952.1.
PIRiF70174.
RefSeqiNP_212733.1. NC_001318.1.
WP_002662611.1. NC_001318.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SP1X-ray2.55A/B1-480[»]
ProteinModelPortaliO51545.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224326.BB_0599.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC66952; AAC66952; BB_0599.
GeneIDi1195446.
KEGGibbu:BB_0599.
PATRICi20557831. VBIBorBur75917_0990.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
KOiK01883.
OMAiAPYKRSP.

Enzyme and pathway databases

BioCyciBBUR224326:G9SO-601-MONOMER.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth. 1 hit.
InterProiIPR015803. Cys-tRNA-ligase.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYC_BORBU
AccessioniPrimary (citable) accession number: O51545
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.