O51419 (NDK_BORBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside diphosphate kinase
Short name=NDK
Short name=NDP kinase
EC=2.7.4.6

Alternative name(s):
Nucleoside-2-P kinase

Gene names

Name:	ndk
Ordered Locus Names:	BB_0463

Organism

Borrelia burgdorferi (Lyme disease spirochete)

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. HAMAP MF_00451
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. HAMAP MF_00451
Cofactor	Magnesium By similarity. HAMAP MF_00451
Subunit structure	Homotetramer By similarity. HAMAP MF_00451
Subcellular location	Cytoplasm By similarity HAMAP MF_00451.
Sequence similarities	Belongs to the NDK family.
Sequence caution	The sequence AAC66829.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 167	167	Nucleoside diphosphate kinase HAMAP MF_00451		PRO_0000136951
Sites
	Active site	132	1	Pros-phosphohistidine intermediate By similarity
	Binding site	11	1	ATP By similarity
	Binding site	92	1	ATP By similarity
	Binding site	98	1	ATP By similarity
	Binding site	109	1	ATP By similarity
	Binding site	129	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O51419 [UniParc]. Last modified September 26, 2001. Version 2. Checksum: 4A8E142C253182B8	FASTA	167	19,159
	10 20 30 40 50 60 MLLQKTLCIV KPDGVRRGLI GDVVSRFERV GLKMVAAKML IVDESLAKKH YLYDDIVFRH 70 80 90 100 110 120 SEAVWNSLIK FISNSPVFTF VVEGVESIEV VRKLCGATEP KLAIPGTIRG DFSYHSFKYS 130 140 150 160 NEKGFSIYNV IHASANEADA MREIPIWFKD NEILNYKRDD ECEHYYC

References

[1]

"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000783 Genomic DNA. Translation: AAC66829.1. Different initiation.
PIR	F70157.
RefSeq	NP_212597.1. NC_001318.1.
3D structure databases
ProteinModelPortal	O51419.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000009005; EBBORP00000008335; EBBORG00000009004.
GeneID	1195309.
GenomeReviews	Gene locus BB_0463 in contig AE000783_GR.
KEGG	bbu:BB0463.
NMPDR	fig|224326.1.peg.847.
PATRIC	20557554. VBIBorBur75917_0856.
TIGR	BB_0463.
Phylogenomic databases
GeneTree	EBGT00050000007252.
HOGENOM	HBG445152.
OMA	ERPFFGA.
PhylomeDB	O51419.
ProtClustDB	PRK14543.
Enzyme and pathway databases
BioCyc	BBUR224326:BB_0463-MONOMER.
Family and domain databases
HAMAP	MF_00451. NDP_kinase. Divergent sequence. [Tree]
InterPro	IPR001564. Nucleoside_diP_kinase. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
KO	K00940.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
SUPFAM	SSF54919. NDK. 1 hit.
PROSITE	PS00469. NDP_KINASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDK_BORBU

Accession

Primary (citable) accession number: O51419

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	September 26, 2001
Last modified:	January 25, 2012
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program