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Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathway:iglycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp), ATP-dependent 6-phosphofructokinase (pfkA)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611Glyceraldehyde 3-phosphateBy similarity
Active sitei109 – 1091Proton donorBy similarity
Metal bindingi110 – 1101Zinc 1; catalyticBy similarity
Metal bindingi144 – 1441Zinc 2By similarity
Metal bindingi174 – 1741Zinc 2By similarity
Metal bindingi226 – 2261Zinc 1; catalyticBy similarity
Binding sitei227 – 2271Dihydroxyacetone phosphate; via amide nitrogenBy similarity
Metal bindingi265 – 2651Zinc 1; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBBUR224326:G9SO-446-MONOMER.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fba
Ordered Locus Names:BB_0445
OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Taxonomic identifieri224326 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliaBorrelia burgdorferi group
ProteomesiUP000001807 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Fructose-bisphosphate aldolasePRO_0000178707Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224326.BB_0445.

Structurei

3D structure databases

ProteinModelPortaliO51401.
SMRiO51401. Positions 3-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni266 – 2683Dihydroxyacetone phosphate bindingBy similarity
Regioni287 – 2904Dihydroxyacetone phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0191.
KOiK01624.
OMAiRMSKMGM.
OrthoDBiEOG69GZPB.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O51401-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVLDKIKPG VVYGKELHFL YEICKKEGFA IPSINCIGTN SINAVLEAAK
60 70 80 90 100
EINSPIMIQF SNSGSAFISG KGLKMEKPQG VSIVGAISGA MHVHLMAEHY
110 120 130 140 150
GVPVVLHTDH CAKNLLPWVE GLLEYGEKYY SQHKKPLFSS HMLDLSEEPI
160 170 180 190 200
KENIEISKKF LERMAKIEMF LEIELGITGG EEDGVDNSDR ALHELFSTPE
210 220 230 240 250
DIYYGYSELL KVSPNFQIAA AFGNVHGVYK PGNVKLTPKV LKDGQDYVIS
260 270 280 290 300
KTGVNMAKPV SYVFHGGSGS TIDEINEALS YGVVKMNIDT DTQWAAWEGV
310 320 330 340 350
LNYYKKNESR LQGQLGDGKD IDIPNKKFYD PRVWLREAEV SMKDRVKIAC

KNLNNINRN
Length:359
Mass (Da):39,981
Last modified:June 1, 1998 - v1
Checksum:iFAF4209478692FCB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21G → S AA sequence (PubMed:2738156).Curated
Sequence conflicti6 – 61K → L AA sequence (PubMed:2738156).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAB91507.1.
PIRiD70155.
RefSeqiNP_212579.1. NC_001318.1.
WP_002557037.1. NC_001318.1.

Genome annotation databases

EnsemblBacteriaiAAB91507; AAB91507; BB_0445.
GeneIDi1195290.
KEGGibbu:BB_0445.
PATRICi20557506. VBIBorBur75917_0836.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000783 Genomic DNA. Translation: AAB91507.1.
PIRiD70155.
RefSeqiNP_212579.1. NC_001318.1.
WP_002557037.1. NC_001318.1.

3D structure databases

ProteinModelPortaliO51401.
SMRiO51401. Positions 3-357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224326.BB_0445.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB91507; AAB91507; BB_0445.
GeneIDi1195290.
KEGGibbu:BB_0445.
PATRICi20557506. VBIBorBur75917_0836.

Phylogenomic databases

eggNOGiCOG0191.
KOiK01624.
OMAiRMSKMGM.
OrthoDBiEOG69GZPB.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
BioCyciBBUR224326:G9SO-446-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
  2. "Identification and characterization of an endoflagellar antigen of Borrelia burgdorferi."
    Coleman J.L., Benach J.L.
    J. Clin. Invest. 84:322-330(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-11.
    Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

Entry informationi

Entry nameiALF_BORBU
AccessioniPrimary (citable) accession number: O51401
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: June 24, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.