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O51401

- ALF_BORBU

UniProt

O51401 - ALF_BORBU

Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Cofactori

    Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei61 – 611Glyceraldehyde 3-phosphateBy similarity
    Active sitei109 – 1091Proton donorBy similarity
    Metal bindingi110 – 1101Zinc 1; catalyticBy similarity
    Metal bindingi144 – 1441Zinc 2By similarity
    Metal bindingi174 – 1741Zinc 2By similarity
    Metal bindingi226 – 2261Zinc 1; catalyticBy similarity
    Binding sitei227 – 2271Dihydroxyacetone phosphate; via amide nitrogenBy similarity
    Metal bindingi265 – 2651Zinc 1; catalyticBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciBBUR224326:G9SO-446-MONOMER.
    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase (EC:4.1.2.13)
    Short name:
    FBP aldolase
    Short name:
    FBPA
    Alternative name(s):
    Fructose-1,6-bisphosphate aldolase
    Gene namesi
    Name:fba
    Ordered Locus Names:BB_0445
    OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
    Taxonomic identifieri224326 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
    ProteomesiUP000001807: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 359359Fructose-bisphosphate aldolasePRO_0000178707Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi224326.BB0445.

    Structurei

    3D structure databases

    ProteinModelPortaliO51401.
    SMRiO51401. Positions 3-357.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni266 – 2683Dihydroxyacetone phosphate bindingBy similarity
    Regioni287 – 2904Dihydroxyacetone phosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0191.
    KOiK01624.
    OMAiHNSLDFV.
    OrthoDBiEOG69GZPB.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR006411. Fruct_bisP_bact.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view]
    PfamiPF01116. F_bP_aldolase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsiTIGR00167. cbbA. 1 hit.
    TIGR01520. FruBisAldo_II_A. 1 hit.
    PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O51401-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGVLDKIKPG VVYGKELHFL YEICKKEGFA IPSINCIGTN SINAVLEAAK    50
    EINSPIMIQF SNSGSAFISG KGLKMEKPQG VSIVGAISGA MHVHLMAEHY 100
    GVPVVLHTDH CAKNLLPWVE GLLEYGEKYY SQHKKPLFSS HMLDLSEEPI 150
    KENIEISKKF LERMAKIEMF LEIELGITGG EEDGVDNSDR ALHELFSTPE 200
    DIYYGYSELL KVSPNFQIAA AFGNVHGVYK PGNVKLTPKV LKDGQDYVIS 250
    KTGVNMAKPV SYVFHGGSGS TIDEINEALS YGVVKMNIDT DTQWAAWEGV 300
    LNYYKKNESR LQGQLGDGKD IDIPNKKFYD PRVWLREAEV SMKDRVKIAC 350
    KNLNNINRN 359
    Length:359
    Mass (Da):39,981
    Last modified:June 1, 1998 - v1
    Checksum:iFAF4209478692FCB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21G → S AA sequence (PubMed:2738156)Curated
    Sequence conflicti6 – 61K → L AA sequence (PubMed:2738156)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000783 Genomic DNA. Translation: AAB91507.1.
    PIRiD70155.
    RefSeqiNP_212579.1. NC_001318.1.

    Genome annotation databases

    EnsemblBacteriaiAAB91507; AAB91507; BB_0445.
    GeneIDi1195290.
    KEGGibbu:BB_0445.
    PATRICi20557506. VBIBorBur75917_0836.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000783 Genomic DNA. Translation: AAB91507.1 .
    PIRi D70155.
    RefSeqi NP_212579.1. NC_001318.1.

    3D structure databases

    ProteinModelPortali O51401.
    SMRi O51401. Positions 3-357.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224326.BB0445.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB91507 ; AAB91507 ; BB_0445 .
    GeneIDi 1195290.
    KEGGi bbu:BB_0445.
    PATRICi 20557506. VBIBorBur75917_0836.

    Phylogenomic databases

    eggNOGi COG0191.
    KOi K01624.
    OMAi HNSLDFV.
    OrthoDBi EOG69GZPB.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .
    BioCyci BBUR224326:G9SO-446-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR006411. Fruct_bisP_bact.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view ]
    Pfami PF01116. F_bP_aldolase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsi TIGR00167. cbbA. 1 hit.
    TIGR01520. FruBisAldo_II_A. 1 hit.
    PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
    2. "Identification and characterization of an endoflagellar antigen of Borrelia burgdorferi."
      Coleman J.L., Benach J.L.
      J. Clin. Invest. 84:322-330(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-11.
      Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.

    Entry informationi

    Entry nameiALF_BORBU
    AccessioniPrimary (citable) accession number: O51401
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3