Reviewed, UniProtKB/Swiss-Prot O51396 (GYRA_BORBU)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	BB_0435

Organism

Borrelia burgdorferi (Lyme disease spirochete) [Complete proteome] [HAMAP]

Taxonomic identifier

139 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	810 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 810

810

DNA gyrase subunit A

PRO_0000145222

Sites

Active site

124

1

O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Experimental info

Sequence conflict

469 – 472

4

IREE → YKRR in CAA78157. Ref.2

Sequence conflict

539

1

F → Y in CAA78157. Ref.2

Secondary structure

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810

Helix Strand Turn

Details...

Beta strand

507 – 513

7

Beta strand

517 – 522

6

Helix

523 – 525

3

Beta strand

547 – 554

8

Beta strand

558 – 563

6

Beta strand

566 – 572

7

Helix

573 – 575

3

Helix

588 – 590

3

Beta strand

600 – 607

8

Beta strand

614 – 619

6

Beta strand

622 – 628

7

Helix

629 – 632

4

Beta strand

651 – 657

7

Beta strand

662 – 667

6

Beta strand

670 – 676

7

Helix

677 – 679

3

Beta strand

701 – 706

6

Beta strand

711 – 717

7

Beta strand

720 – 726

7

Helix

727 – 729

3

Turn

748 – 750

3

Beta strand

752 – 759

8

Beta strand

764 – 769

6

Beta strand

773 – 778

6

Helix

779 – 781

3

Beta strand

802 – 807

6

Sequences

	Sequence		Length	Mass (Da)
	O51396-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 395F3930F0EF1650	FASTA	810	91,379
	10 20 30 40 50 60 MAVGENKEQI LNVRIEDEIK TSYLNYAMSV IVSRALPDVR DGLKPVHRRI LYSMYEMGLR 70 80 90 100 110 120 SDKAFKKAGR IVGDVLGKYH PHGDQSIYDA LVRLAQDFSL RYPVIRGQGN FGSIDGDPPA 130 140 150 160 170 180 AMRYTEAKME KITEYIVKDI DKETVNFKSN YDDSLSEPEI MPSSFPFLLV NGSSGIAVGM 190 200 210 220 230 240 ATNMAPHNLR EICDAIVYML DNENASIFDL LKIVKGPDFP TFGEIVYNDN LIKAYKTGKG 250 260 270 280 290 300 SVVIRARYHI EERAEDRNAI IVTEIPYTVN KSALLMKVAL LAKEEKLEGL LDIRDESDRE 310 320 330 340 350 360 GIRIVLEVKR GFDPHVIMNL LYEYTEFKKH FSINNLALVN GIPKQLNLEE LLFEFIEHRK 370 380 390 400 410 420 NIIERRIEFD LRKAKEKAHV LEGLNIALNN IDEVIKIIKS SKLAKDARER LVSNFGLSEI 430 440 450 460 470 480 QANSVLDMRL QKLTALEIFK LEEELNILLS LIKDYEDILL NPVRIINIIR EETINLGLKF 490 500 510 520 530 540 GDERRTKIIY DEEVLKTSMS DLMQKENIVV MLTKKGFLKR LSQNEYKLQG TGGKGLSSFD 550 560 570 580 590 600 LNDGDEIVIA LCVNTHDYLF MISNEGKLYL INAYEIKDSS RASKGQNISE LINLGDQEEI 610 620 630 640 650 660 LTIKNSKDLT DDAYLLLTTA SGKIARFEST DFKAVKSRGV IVIKLNDKDF VTSAEIVFKD 670 680 690 700 710 720 EKVICLSKKG SAFIFNSRDV RLTNRGTQGV CGMKLKEGDL FVKVLSVKEN PYLLIVSENG 730 740 750 760 770 780 YGKRLNMSKI SELKRGATGY TSYKKSDKKA GSVVDAIAVS EDDEILLVSK RSKALRTVAG 790 800 810 KVSEQGKDAR GIQVLFLDND SLVSVSKFIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi." Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L., Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B., Smith H.O., Venter J.C. Nature 390:580-586(1997) [PubMed: 9403685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680.
[2]	"Mapping of genes on the linear chromosome of the bacterium Borrelia burgdorferi: possible locations for its origin of replication." Old I.G., Macdougall J.H., Saint-Girons I., Davidson B.E. FEMS Microbiol. Lett. 78:245-250(1992) [PubMed: 1490605] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 450-545. Strain: 212.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000783 Genomic DNA. Translation: AAC66803.1.
Z12165 Genomic DNA. Translation: CAA78157.1.

PIR

B70154.

RefSeq

NP_212569.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SUU	X-ray	1.75	A	499-810	[»]

ModBase

Search...

Genome annotation databases

GeneID

1195280.

GenomeReviews

Gene locus BB_0435 in contig AE000783_GR.

KEGG

bbu:BB0435.

NMPDR

fig|224326.1.peg.819.

TIGR

BB_0435.

Phylogenomic databases

HOGENOM

O51396.

OMA

SEIQANS.

Enzyme and pathway databases

BioCyc

BBUR224326:BB_0435-MON.

BRENDA

5.99.1.3. 142596.

Family and domain databases

InterPro

IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
[Graphical view]

Gene3D

G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit.
G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.

Pfam

PF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]

ProDom

PD000742. DNA_topoisoIV. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00434. TOP4c. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01063. gyrA. 1 hit.

ProtoNet

Search...

Entry information

Entry name

GYRA_BORBU

Accession

Primary (citable) accession number: O51396
Secondary accession number(s): Q44931

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)