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Protein

Proline--tRNA ligase

Gene

proS

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). Can inadvertently accommodate and process cysteine.

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).

Kineticsi

  1. KM=0.17 mM for proline1 Publication
  2. KM=0.18 mM for cysteine1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBBUR224326:G9SO-401-MONOMER.
    SABIO-RKO51363.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline--tRNA ligase (EC:6.1.1.15)
    Alternative name(s):
    Prolyl-tRNA synthetase
    Short name:
    ProRS
    Gene namesi
    Name:proS
    Ordered Locus Names:BB_0402
    OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
    Taxonomic identifieri224326 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliella
    Proteomesi
    • UP000001807 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 488488Proline--tRNA ligasePRO_0000139321Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi224326.BB_0402.

    Structurei

    3D structure databases

    ProteinModelPortaliO51363.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4107R1M. Bacteria.
    COG0442. LUCA.
    KOiK01881.
    OMAiVYWGKAY.

    Family and domain databases

    CDDicd00778. ProRS_core_arch_euk. 1 hit.
    Gene3Di3.30.110.30. 1 hit.
    3.40.50.800. 1 hit.
    HAMAPiMF_01571. Pro_tRNA_synth_type3. 1 hit.
    InterProiIPR002314. aa-tRNA-synt_IIb.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004499. Pro-tRNA-ligase_IIa_arc-type.
    IPR016061. Pro-tRNA_ligase_II_C.
    IPR017449. Pro-tRNA_synth_II.
    IPR033721. ProRS_core_arch_euk.
    [Graphical view]
    PANTHERiPTHR11451:SF6. PTHR11451:SF6. 1 hit.
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF09180. ProRS-C_1. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view]
    PRINTSiPR01046. TRNASYNTHPRO.
    SMARTiSM00946. ProRS-C_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF52954. SSF52954. 1 hit.
    SSF64586. SSF64586. 1 hit.
    TIGRFAMsiTIGR00408. proS_fam_I. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O51363-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSDFIASKED DYSKWYLDIV QKAKLADYSP VKGCMVIMPY GYSIWSKIQS
    60 70 80 90 100
    ILDKKFKETG HENAYFPMLI PYSFLEREKD HIDGFSPEFA IIKDAGGESL
    110 120 130 140 150
    AEPLVLRPTS ETIIWNMYSK WIKSYRDLPL KINQWANVVR WEKRTRPFLR
    160 170 180 190 200
    TTEFLWQEGH TAHATEEEAL EETLLILDVY KRFIEDYLAI PVFCGKKSEK
    210 220 230 240 250
    EKFAGAVSTY SIEALMQDKK ALQAATSHYL GLNFAKAFDV KFQDKDGKMR
    260 270 280 290 300
    HVFASSWGVS TRLIGALIMV HSDEKGLVLP PRIAPIEIIV IPIFKKEDEI
    310 320 330 340 350
    NKKILDYSDC VVDALKKAEF RVEIDKDVRS SPGFRFSSAE FKGIPIRLEV
    360 370 380 390 400
    GINDVLLNSV TISRRDKDRK FKYQISLDSL ISKVKVELDL MQKDLFQRAL
    410 420 430 440 450
    NFRILNTKEI FRSSKDSYET FKAYVNDYSG FVLSCWCGSL NCENIIKNET
    460 470 480
    KATIRCIPDD FKARDLTGMT CIYCSSKAKY FVLFAKSY
    Length:488
    Mass (Da):56,311
    Last modified:June 1, 1998 - v1
    Checksum:i96E036EA479FFB60
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000783 Genomic DNA. Translation: AAC66767.1.
    PIRiA70150.
    RefSeqiNP_212536.1. NC_001318.1.
    WP_010889744.1. NC_001318.1.

    Genome annotation databases

    EnsemblBacteriaiAAC66767; AAC66767; BB_0402.
    GeneIDi1195240.
    KEGGibbu:BB_0402.
    PATRICi20557416. VBIBorBur75917_0796.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000783 Genomic DNA. Translation: AAC66767.1.
    PIRiA70150.
    RefSeqiNP_212536.1. NC_001318.1.
    WP_010889744.1. NC_001318.1.

    3D structure databases

    ProteinModelPortaliO51363.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224326.BB_0402.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC66767; AAC66767; BB_0402.
    GeneIDi1195240.
    KEGGibbu:BB_0402.
    PATRICi20557416. VBIBorBur75917_0796.

    Phylogenomic databases

    eggNOGiENOG4107R1M. Bacteria.
    COG0442. LUCA.
    KOiK01881.
    OMAiVYWGKAY.

    Enzyme and pathway databases

    BioCyciBBUR224326:G9SO-401-MONOMER.
    SABIO-RKO51363.

    Family and domain databases

    CDDicd00778. ProRS_core_arch_euk. 1 hit.
    Gene3Di3.30.110.30. 1 hit.
    3.40.50.800. 1 hit.
    HAMAPiMF_01571. Pro_tRNA_synth_type3. 1 hit.
    InterProiIPR002314. aa-tRNA-synt_IIb.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004499. Pro-tRNA-ligase_IIa_arc-type.
    IPR016061. Pro-tRNA_ligase_II_C.
    IPR017449. Pro-tRNA_synth_II.
    IPR033721. ProRS_core_arch_euk.
    [Graphical view]
    PANTHERiPTHR11451:SF6. PTHR11451:SF6. 1 hit.
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF09180. ProRS-C_1. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view]
    PRINTSiPR01046. TRNASYNTHPRO.
    SMARTiSM00946. ProRS-C_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF52954. SSF52954. 1 hit.
    SSF64586. SSF64586. 1 hit.
    TIGRFAMsiTIGR00408. proS_fam_I. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiSYP_BORBU
    AccessioniPrimary (citable) accession number: O51363
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: June 1, 1998
    Last modified: September 7, 2016
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.