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Protein

Proline--tRNA ligase

Gene

proS

Organism
Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). Can inadvertently accommodate and process cysteine.

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).

Kineticsi

  1. KM=0.17 mM for proline1 Publication
  2. KM=0.18 mM for cysteine1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAminoacyl-tRNA synthetase, Ligase
    Biological processProtein biosynthesis
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKO51363

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline--tRNA ligase (EC:6.1.1.15)
    Alternative name(s):
    Prolyl-tRNA synthetase
    Short name:
    ProRS
    Gene namesi
    Name:proS
    Ordered Locus Names:BB_0402
    OrganismiBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
    Taxonomic identifieri224326 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliella
    Proteomesi
    • UP000001807 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001393211 – 488Proline--tRNA ligaseAdd BLAST488

    Proteomic databases

    PRIDEiO51363

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi224326.BB_0402

    Structurei

    3D structure databases

    ProteinModelPortaliO51363
    SMRiO51363
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4107R1M Bacteria
    COG0442 LUCA
    KOiK01881
    OMAiEVYWVTH

    Family and domain databases

    CDDicd00778 ProRS_core_arch_euk, 1 hit
    Gene3Di3.30.110.30, 1 hit
    3.40.50.800, 1 hit
    HAMAPiMF_01571 Pro_tRNA_synth_type3, 1 hit
    InterProiView protein in InterPro
    IPR002314 aa-tRNA-synt_IIb
    IPR006195 aa-tRNA-synth_II
    IPR004154 Anticodon-bd
    IPR036621 Anticodon-bd_dom_sf
    IPR002316 Pro-tRNA-ligase_IIa
    IPR004499 Pro-tRNA-ligase_IIa_arc-type
    IPR016061 Pro-tRNA_ligase_II_C
    IPR017449 Pro-tRNA_synth_II
    IPR033721 ProRS_core_arch_euk
    PfamiView protein in Pfam
    PF03129 HGTP_anticodon, 1 hit
    PF09180 ProRS-C_1, 1 hit
    PF00587 tRNA-synt_2b, 1 hit
    PRINTSiPR01046 TRNASYNTHPRO
    SMARTiView protein in SMART
    SM00946 ProRS-C_1, 1 hit
    SUPFAMiSSF64586 SSF64586, 1 hit
    TIGRFAMsiTIGR00408 proS_fam_I, 1 hit
    PROSITEiView protein in PROSITE
    PS50862 AA_TRNA_LIGASE_II, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O51363-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSDFIASKED DYSKWYLDIV QKAKLADYSP VKGCMVIMPY GYSIWSKIQS
    60 70 80 90 100
    ILDKKFKETG HENAYFPMLI PYSFLEREKD HIDGFSPEFA IIKDAGGESL
    110 120 130 140 150
    AEPLVLRPTS ETIIWNMYSK WIKSYRDLPL KINQWANVVR WEKRTRPFLR
    160 170 180 190 200
    TTEFLWQEGH TAHATEEEAL EETLLILDVY KRFIEDYLAI PVFCGKKSEK
    210 220 230 240 250
    EKFAGAVSTY SIEALMQDKK ALQAATSHYL GLNFAKAFDV KFQDKDGKMR
    260 270 280 290 300
    HVFASSWGVS TRLIGALIMV HSDEKGLVLP PRIAPIEIIV IPIFKKEDEI
    310 320 330 340 350
    NKKILDYSDC VVDALKKAEF RVEIDKDVRS SPGFRFSSAE FKGIPIRLEV
    360 370 380 390 400
    GINDVLLNSV TISRRDKDRK FKYQISLDSL ISKVKVELDL MQKDLFQRAL
    410 420 430 440 450
    NFRILNTKEI FRSSKDSYET FKAYVNDYSG FVLSCWCGSL NCENIIKNET
    460 470 480
    KATIRCIPDD FKARDLTGMT CIYCSSKAKY FVLFAKSY
    Length:488
    Mass (Da):56,311
    Last modified:June 1, 1998 - v1
    Checksum:i96E036EA479FFB60
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000783 Genomic DNA Translation: AAC66767.1
    PIRiA70150
    RefSeqiNP_212536.1, NC_001318.1
    WP_010889744.1, NC_001318.1

    Genome annotation databases

    EnsemblBacteriaiAAC66767; AAC66767; BB_0402
    GeneIDi1195240
    KEGGibbu:BB_0402
    PATRICifig|224326.49.peg.796

    Similar proteinsi

    Entry informationi

    Entry nameiSYP_BORBU
    AccessioniPrimary (citable) accession number: O51363
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: June 1, 1998
    Last modified: March 28, 2018
    This is version 123 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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